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Literature summary extracted from

  • Barnes, K.; Ingram, J.; Kenny, A.J.
    Proteins of the kidney microvillar membrane. Structural and immunochemical properties of rat endopeptidase-2 and its immunohistochemical localization in tissues of rat and mouse (1989), Biochem. J., 264, 335-346.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.18 membrane renal brush border membrane Mus musculus 16020
-
3.4.24.18 membrane renal brush border membrane Rattus norvegicus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.18 80000
-
 x * 80000, SDS-PAGE, reducing conditions Mus musculus
3.4.24.18 80000
-
 x * 80000, SDS-PAGE, reducing conditions Rattus norvegicus
3.4.24.18 90000
-
 x * 90000, mouse, SDS-PAGE, reducing conditions Mus musculus
3.4.24.18 90000
-
 x * 90000, mouse, SDS-PAGE, reducing conditions Rattus norvegicus
3.4.24.18 220000
-
 rat, SDS-PAGE, non-reducing conditions Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.18 Mus musculus
-
 meprin A, from male BALB/c
-
3.4.24.18 Rattus norvegicus
-
 endopeptidase-2, male Wistar
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.24.18 glycoprotein 30% Asp-linked carbohydrates, no glycolipid anchor Mus musculus
3.4.24.18 glycoprotein 30% Asp-linked carbohydrates, no glycolipid anchor Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.18 immunoaffinity chromatography Rattus norvegicus
3.4.24.18 partial Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.18 intestine from duodenum to rectum Mus musculus
-
3.4.24.18 intestine from duodenum to rectum Rattus norvegicus
-
3.4.24.18 kidney lumen of proximal tubule, juxtamedullary nephrons Mus musculus
-
3.4.24.18 kidney lumen of proximal tubule, juxtamedullary nephrons Rattus norvegicus
-
3.4.24.18 additional information tissue distribution (immuno-peroxidase staining), not in brain or spinal cord Mus musculus
-
3.4.24.18 additional information tissue distribution (immuno-peroxidase staining), not in brain or spinal cord Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.18 azocasein + H2O better substrate for mouse enzyme than for rat enzyme Mus musculus fragments of azocasein
-
 ?
3.4.24.18 azocasein + H2O better substrate for mouse enzyme than for rat enzyme Rattus norvegicus fragments of azocasein
-
 ?
3.4.24.18 Insulin B-chain + H2O
-
 Mus musculus Hydrolyzed insulin B-chain
-
 ?
3.4.24.18 Insulin B-chain + H2O I125-iodinated Rattus norvegicus Hydrolyzed insulin B-chain
-
 ?
3.4.24.18 N-Benzoyl-L-tyrosyl-4-aminobenzoate + H2O
-
 Rattus norvegicus N-Benzoyl-L-tyrosine + 4-aminobenzoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.18 oligomer x * 80000, SDS-PAGE, reducing conditions Mus musculus
3.4.24.18 oligomer x * 80000, SDS-PAGE, reducing conditions Rattus norvegicus
3.4.24.18 oligomer x * 90000, mouse, SDS-PAGE, reducing conditions Mus musculus
3.4.24.18 oligomer x * 90000, mouse, SDS-PAGE, reducing conditions Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.4.24.18 More meprin A from mouse, endopeptidase-2 from rat and PPH from human are closely related enzymes of the astacin family Mus musculus
3.4.24.18 More meprin A from mouse, endopeptidase-2 from rat and PPH from human are closely related enzymes of the astacin family Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.18 37
-
 assay at Mus musculus
3.4.24.18 37
-
 assay at Rattus norvegicus