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Literature summary extracted from
- Effect of pH on the activities of penicillopepsin and Rhizopus pepsin and a proposal for the productive substrate binding mode in penicillopepsin (1984), Biochemistry, 23, 635-643.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.23.20 | 0.004 | - |
Leu-Ser-(4-nitro)Phe-Nle-Ala-Leu methyl ester | pH 5.5 | Penicillium janthinellum |  |
| 3.4.23.20 | 0.01 | - |
Leu-Ser-(4-nitro)Phe-Nle-Ala-Leu methyl ester | pH 3.5 | Penicillium janthinellum | |
| 3.4.23.20 | 0.22 | - |
Ac-Lys 4-nitrophenyl amide | - |
Penicillium janthinellum | |
| 3.4.23.20 | 0.44 | - |
Ac-Ala-Ala-Lys-(4-nitro)Phe-Ala-Ala amide | - |
Penicillium janthinellum | |
| 3.4.23.21 | 0.23 | - |
Ac-Ala-Ala-Lys-(4-nitro)Phe-Ala-Ala | pH 6.0 | Rhizopus sp. | |
| 3.4.23.21 | 1.1 | - |
Ac-Ala-Ala-Lys-(4-nitro)Phe-Ala-Ala | pH 2.0 | Rhizopus sp. | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.23.20 | Penicillium janthinellum | - |
- |
- |
| 3.4.23.21 | Rhizopus sp. | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.20 | Ac-Ala-Ala-Lys-(4-nitro)Phe-Ala-Ala amide + H2O | - |
Penicillium janthinellum | ? | - |
? | |
| 3.4.23.20 | Ac-Lys 4-nitrophenyl amide + H2O | - |
Penicillium janthinellum | ? | - |
? | |
| 3.4.23.20 | Leu-Ser-(4-nitro)Phe-Nle-Ala-Leu methyl ester + H2O | - |
Penicillium janthinellum | ? | - |
? | |
| 3.4.23.21 | Ac-Ala-Ala-(4-nitro)Phe-Ala-Ala + H2O | - |
Rhizopus sp. | ? | - |
? | |
| 3.4.23.21 | Ac-Ala-Ala-Lys-(4-nitro)Phe-Ala-Ala + H2O | - |
Rhizopus sp. | Ac-Ala-Ala-Lys + (4-nitro)Phe-Ala-Ala | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.21 | 25 | - |
assay at | Rhizopus sp. |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.23.20 | 0.01 | - |
Ac-Lys 4-nitrophenyl amide | pH 5.5 | Penicillium janthinellum | |
| 3.4.23.20 | 6.4 | - |
Leu-Ser-(4-nitro)Phe-Nle-Ala-Leu methyl ester | pH 3.5 | Penicillium janthinellum | |
| 3.4.23.20 | 11.4 | - |
Leu-Ser-(4-nitro)Phe-Nle-Ala-Leu methyl ester | pH 5.5 | Penicillium janthinellum | |
| 3.4.23.20 | 28.8 | - |
Ac-Ala-Ala-Lys-(4-nitro)Phe-Ala-Ala amide | pH 3.5 | Penicillium janthinellum | |
| 3.4.23.20 | 36 | - |
Ac-Ala-Ala-Lys-(4-nitro)Phe-Ala-Ala amide | pH 5.5 | Penicillium janthinellum | |
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