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Literature summary extracted from

  • Tsujita, Y.; Endo, A.
    Purification and characterization of the two molecular forms of membrane acid protease from Aspergillus oryzae (1978), Eur. J. Biochem., 84, 347-353.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.23.18 cholesterol activation, acetone-washed membrane-bound enzyme Aspergillus oryzae
3.4.23.18 Diglycerides activation, acetone-washed membrane-bound enzyme Aspergillus oryzae
3.4.23.18 monoglycerides activation, acetone-washed membrane-bound enzyme Aspergillus oryzae
3.4.23.18 additional information no activation of membrane-bound enzyme by triglycerides or 1,3-dipalmitin Aspergillus oryzae
3.4.23.18 Phospholipids activation, acetone-washed membrane-bound enzyme Aspergillus oryzae
3.4.23.18 Triton X-100 activation, acetone-washed membrane-bound enzyme Aspergillus oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.23.18 additional information diisopropyl phosphofluoridate; monoiodoacetate, 1,10-phenanthroline, 6-aminohexanoate; PCMB Aspergillus oryzae
3.4.23.18 N-bromosuccinimide not Aspergillus oryzae
3.4.23.18 SDS 3.3 mM Aspergillus oryzae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.23.18 membrane
-
 Aspergillus oryzae 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.23.18 100000
-
 Aspergillus oryzae, enzyme form M2, gel filtration Aspergillus oryzae
3.4.23.18 230000
-
 Aspergillus oryzae, Triton X-100 treated enzyme, gel filtration Aspergillus oryzae
3.4.23.18 2000000
-
 Aspergillus oryzae, enzyme form M1, gel filtration Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.18 Aspergillus oryzae
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.23.18 glycoprotein 80.5% (enzyme form M1), 52.5% (enzyme form M2) Aspergillus oryzae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.23.18 several isoforms (membrane-bound M1 and M2) Aspergillus oryzae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.23.18 mycelium
-
 Aspergillus oryzae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.23.18 143
-
 enzyme form M1, in the presence of Triton X-100 Aspergillus oryzae
3.4.23.18 867
-
 enzyme form M2, in the presence of Triton X-100 Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.18 Cytochrome c + H2O
-
 Aspergillus oryzae ?
-
 ?
3.4.23.18 Hemoglobin + H2O urea-denatured hemoglobin Aspergillus oryzae ?
-
 ?
3.4.23.18 Trypsinogen + H2O
-
 Aspergillus oryzae ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.23.18 55
-
 10 min, in 50 mM sodium acetate buffer, pH 5 Aspergillus oryzae
3.4.23.18 55
-
 isozyme M1 retains more than 95% of its original activity, M2 is completely inactivated Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.18 3.2
-
 cytochrome c Aspergillus oryzae
3.4.23.18 4.5
-
 urea-denatured hemoglobin Aspergillus oryzae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.23.18 3 6 stable in this range, complete inactivation below 2 and above 7 Aspergillus oryzae