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Literature summary extracted from

  • Loh, Y.P.; Parish, D.C.; Tuteja, R.
    Purification and characterization of a paired basic residue-specific pro-opiomelanocortin converting enzyme from bovine pituitary intermediate lobe secretory vesicles (1985), J. Biol. Chem., 260, 7194-7205.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.23.17 dithiodipyridine
-
 Bos taurus
3.4.23.17 leupeptin
-
 Bos taurus
3.4.23.17 additional information not: diisopropyl fluorophosphate; phenylmethane sulfonylfluoride Bos taurus
3.4.23.17 pepstatin A
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.23.17 70000
-
 x * 70000, bovine Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.23.17 Pro-opiomelanocortin + H2O Bos taurus may function as a pro-opiomelanocortin processing enzyme in the pituitary intermediate lobe ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.17 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.23.17 glycoprotein
-
 Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.23.17
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.23.17 hypophysis intermediate Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
3.4.23.17 -20°C, pH 7.4, loss of activity 2-3 weeks after purification Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.17 additional information poorly cleaved: benzyloxycarbonyl-Val-Lys-Lys-Arg 4-methoxy-2-naphthylamide Bos taurus ?
-
 ?
3.4.23.17 additional information not: Lys-Lys-Arg-Arg residues in ACTH1-39 Bos taurus ?
-
 ?
3.4.23.17 Pro-insulin + H2O
-
 Bos taurus Insulin A-chain + insulin B-chain enzyme cleaves pro-insulin at the Lys-Arg pair to yield the A chain of insulin and at the Arg-Arg pair to yield the B-chain and the A-chain linked to C-chain (A-C) intermediate ?
3.4.23.17 Pro-opiomelanocortin + H2O cleaves the peptide bonds either between the Lys and Arg or on the carboxyl side of the Arg at Lys-Arg pairs of pro-opiomelanocortin Bos taurus Adrenocorticotropin + beta-lipotropin + beta-endorphin + MW 16000 NH2-terminal glycopeptide ?
3.4.23.17 Pro-opiomelanocortin + H2O cleaves the peptide bonds either between the Lys and Arg or on the carboxyl side of the Arg at Lys-Arg pairs of pro-opiomelanocortin Bos taurus Adrenocorticotropin + beta-lipotropin ACTH of MW 21000-23000, 13000 and 4500 ?
3.4.23.17 Pro-opiomelanocortin + H2O may function as a pro-opiomelanocortin processing enzyme in the pituitary intermediate lobe Bos taurus ?
-
 ?
3.4.23.17 Small synthetic peptides with paired basic residues + H2O
-
 Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.23.17 ? SDS-PAGE Bos taurus
3.4.23.17 ? x * 70000, bovine Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.23.17 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.17 4 4.5
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.23.17 3 6 3: about 60% of activity maximum Bos taurus
3.4.23.17 3 6 6: about 70% of activity maximum Bos taurus