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Literature summary extracted from

  • Chen, Z.; Potempa, J.; Polanowski, A.; Wikstrom, M.; Travis, J.
    Purification and characterization of a 50-kDa cysteine proteinase (gingipain) from Porphyromonas gingivalis (1992), J. Biol. Chem., 267, 18896-18901.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.37 cysteine required for full amidolytic activity Porphyromonas gingivalis

General Stability

EC Number General Stability Organism
3.4.22.37 Ca2+ stabilizes Porphyromonas gingivalis
3.4.22.37 Irreversible loss of activity during lyophilization Porphyromonas gingivalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.37 EDTA completely reversed by addition of excess Ca2+ Porphyromonas gingivalis
3.4.22.37 EGTA completely reversed by addition of excess Ca2+ Porphyromonas gingivalis
3.4.22.37 iodoacetamide
-
 Porphyromonas gingivalis
3.4.22.37 iodoacetic acid
-
 Porphyromonas gingivalis
3.4.22.37 additional information resistant to inhibition by proteinase inhibitors in human plasma Porphyromonas gingivalis
3.4.22.37 N-Chlorosuccinimide
-
 Porphyromonas gingivalis
3.4.22.37 NEM
-
 Porphyromonas gingivalis
3.4.22.37 p-aminobenzamidine
-
 Porphyromonas gingivalis
3.4.22.37 tosyl-L-Lys chloromethyl ketone i.e. TLCK Porphyromonas gingivalis
3.4.22.37 tosyl-L-Phe chloromethyl ketone i.e. TPCK Porphyromonas gingivalis
3.4.22.37 ZnCl2
-
 Porphyromonas gingivalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.37 Ca2+ may play a role in enzyme catalysis Porphyromonas gingivalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.37 44000
-
 Porphyromonas gingivalis, gel filtration Porphyromonas gingivalis
3.4.22.37 50000
-
 1 * 50000, Porphyromonas gingivalis, SDS-PAGE Porphyromonas gingivalis

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.37 Porphyromonas gingivalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.37
-
 Porphyromonas gingivalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.37 culture medium
-
 Porphyromonas gingivalis
-

Storage Stability

EC Number Storage Stability Organism
3.4.22.37 -20°C, stable for several months Porphyromonas gingivalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.37 benzoyl-Arg 4-nitroanilide + H2O
-
 Porphyromonas gingivalis benzoyl-Arg + 4-nitroaniline
-
 ?
3.4.22.37 Benzoyl-Ile-Glu-(gamma-ornithyl)-Gly-Arg 4-nitroanilide + H2O
-
 Porphyromonas gingivalis ?
-
 ?
3.4.22.37 D-Ile-Pro-Arg 4-nitroanilide + H2O
-
 Porphyromonas gingivalis ?
-
 ?
3.4.22.37 D-Phe-Pip-Arg 4-nitroanilide + H2O
-
 Porphyromonas gingivalis ?
-
 ?
3.4.22.37 Insulin B-chain + H2O specific cleavage of Arg-+- Porphyromonas gingivalis ?
-
 ?
3.4.22.37 Mellitin + H2O specific cleavage of Arg-+- Porphyromonas gingivalis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.37 monomer 1 * 50000, Porphyromonas gingivalis, SDS-PAGE Porphyromonas gingivalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.37 7.5
-
 in presence of glycylglycine Porphyromonas gingivalis