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Literature summary extracted from
- The two cysteine endopeptidases of legume seeds: purification and characterization by use of specific fluorometric assays (1993), Arch. Biochem. Biophys., 303, 208-213.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.22.34 | Benzyloxycarbonyl-Phe-Ala-CHN2 | - |
Vigna aconitifolia |  |
| 3.4.22.34 | cystatin | - |
Vigna aconitifolia | |
| 3.4.22.34 | iodoacetamide | - |
Vigna aconitifolia | |
| 3.4.22.34 | iodoacetate | - |
Vigna aconitifolia | |
| 3.4.22.34 | L-3-carboxy-2,3-trans-epoxypropionyl-leucyl-amido(4-guanidino)butane | i.e. E-64, not | Vigna aconitifolia | |
| 3.4.22.34 | leupeptin | - |
Vigna aconitifolia | |
| 3.4.22.34 | additional information | - |
Vigna aconitifolia | |
| 3.4.22.34 | tosyl-Phe-CH2Cl | - |
Vigna aconitifolia | |
| 3.4.22.B47 | cystatin | reversible inhibition | Vigna aconitifolia | |
| 3.4.22.B47 | E-64 | - |
Vigna aconitifolia | |
| 3.4.22.B47 | iodoacetamide | exceptionally low rat constant for inhibition | Vigna aconitifolia | |
| 3.4.22.B47 | iodoacetate | exceptionally low rat constant for inhibition | Vigna aconitifolia | |
| 3.4.22.B47 | leupeptin | reversible inhibition | Vigna aconitifolia | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.B47 | 27000 | - |
x * 27000, SDS-PAGE | Vigna aconitifolia |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.22.34 | Vigna aconitifolia | - |
- |
- |
| 3.4.22.B47 | Vigna aconitifolia | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.22.B47 | additional information | the enzyme is not a glycoprotein | Vigna aconitifolia |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.22.34 | - |
Vigna aconitifolia |
| 3.4.22.B47 | - |
Vigna aconitifolia |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.22.34 | seed | activity increases during germination | Vigna aconitifolia | - |
| 3.4.22.B47 | seed | activity increases during germination | Vigna aconitifolia | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.34 | additional information | - |
- |
Vigna aconitifolia |
| 3.4.22.B47 | 890 | - |
- |
Vigna aconitifolia |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.34 | azocasein + H2O | - |
Vigna aconitifolia | fragments of azocasein | - |
? | |
| 3.4.22.34 | Benzyloxycarbonyl-Ala-Ala-Asn 4-methylcoumarin 7-amide + H2O | - |
Vigna aconitifolia | ? | - |
? | |
| 3.4.22.34 | Benzyloxycarbonyl-Ala-Pro-Asn 4-methylcoumarin 7-amide + H2O | - |
Vigna aconitifolia | ? | - |
? | |
| 3.4.22.34 | Benzyloxycarbonyl-Ala-Pro-Tyr-Asn 4-methylcoumarin 7-amide + H2O | - |
Vigna aconitifolia | ? | - |
? | |
| 3.4.22.34 | Benzyloxycarbonyl-Pro-Ala-Asn 4-methylcoumarin 7-amide + H2O | - |
Vigna aconitifolia | ? | - |
? | |
| 3.4.22.34 | Gelatin + H2O | - |
Vigna aconitifolia | Hydrolyzed gelatin | - |
? | |
| 3.4.22.B47 | azocasein + H2O | - |
Vigna aconitifolia | ? | - |
? | |
| 3.4.22.B47 | benzoyl-Phe-Val-Arg-7-amido-4-methylcoumarin + H2O | 4.4% of the activity with benzyloxycarbonyl-Phe-Arg-7-(4-methyl)coumarylamide | Vigna aconitifolia | benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.22.B47 | benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O | 10% of the activity with benzyloxycarbonyl-Phe-Arg-7-(4-methyl)coumarylamide | Vigna aconitifolia | benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.22.B47 | benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Vigna aconitifolia | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.22.B47 | Gelatin + H2O | - |
Vigna aconitifolia | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.22.B47 | ? | x * 27000, SDS-PAGE | Vigna aconitifolia |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.34 | 26.5 | - |
pH 3.0-7.0, very stable | Vigna aconitifolia |
| 3.4.22.B47 | 26.5 | - |
very stable during incubation in the range pH 3.0-7.0 | Vigna aconitifolia |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.34 | 5 | - |
benzyloxycarbonyl-Ala-Ala-Asn 4-methylcoumarin 7-amide | Vigna aconitifolia |
| 3.4.22.B47 | 5.5 | - |
reaction with azocasein | Vigna aconitifolia |
| 3.4.22.B47 | 7.5 | - |
activity with benzyloxycarbonyl-Phe-Arg-7-(4-methyl)coumarylamide | Vigna aconitifolia |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.B47 | 3.5 | 7.5 | pH 3.5: about 40% of maximal activity, pH 7.5: about 65% of maximal activity, reaction with casein | Vigna aconitifolia |
| 3.4.22.B47 | 4.5 | 8 | pH 4.5: about 35% of maximal activity, pH 8.0: about 60% of maximal activity, reaction with benzyloxycarbonyl-Phe-Arg-7-(4-methyl)coumarylamide | Vigna aconitifolia |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.34 | 3 | 7 | 26.5°C, very stable | Vigna aconitifolia |
| 3.4.22.B47 | 3 | 7 | stable at 26.5°C | Vigna aconitifolia |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.22.B47 | 0.0000075 | - |
leupeptin | 26°C, pH 6.0 | Vigna aconitifolia | |
| 3.4.22.B47 | 0.00003 | - |
cystatin | 26°C, pH 6.0 | Vigna aconitifolia |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.4.22.B47 | Vigna aconitifolia | - |
4.7 | 4.6 |
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