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Literature summary extracted from

  • Minami, Y.; Doi, E.; Hata, T.
    Fractionation, purification, and some properties of proteolytic enzymes from stem bromelain (1971), Agric. Biol. Chem., 35, 1419-1430.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.32 2,3-dimercapto-1-propanol not as effective in activation as cysteine Ananas comosus
3.4.22.32 cysteine inactive unless treated with Ananas comosus
3.4.22.32 DTT not as effective in activation as cysteine Ananas comosus
3.4.22.32 Thioglycol not as effective in activation as cysteine Ananas comosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.32 HgCl2
-
 Ananas comosus
3.4.22.32 iodoacetic acid
-
 Ananas comosus
3.4.22.32 PCMB
-
 Ananas comosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.32 57
-
 benzoyl-L-Arg ethyl ester acidic enzyme form Ananas comosus
3.4.22.32 67
-
 benzoyl-L-Arg amide acidic enzyme form Ananas comosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.32 Ananas comosus
-
 2 enzyme forms: pI 9.45 (basic form), pI 4.7 (acidic form)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.32 2 enzyme forms with pI of 9.45 (basic form) and pI 4.7 (acidic form) Ananas comosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.32 stem
-
 Ananas comosus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.32 Benzoyl-L-Arg amide + H2O
-
 Ananas comosus Benzoyl-L-Arg + NH3
-
 ?
3.4.22.32 Benzoyl-L-Arg ethyl ester + H2O
-
 Ananas comosus Benzoyl-L-Arg + ethanol
-
 ?
3.4.22.32 casein + H2O
-
 Ananas comosus hydrolyzed casein
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.32 45
-
 pH 7, 30 min, stable up to Ananas comosus
3.4.22.32 50
-
 pH 5, 60 min, stable Ananas comosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.32 7 8.5 casein Ananas comosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.32 5 10 5: about 40% of activity maximum, 10.0: about 70% of activity maximum Ananas comosus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.32 3 9 4°C, 24 h, stable Ananas comosus
3.4.22.32 5
-
 50°C, 60 min, stable Ananas comosus