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Literature summary extracted from

  • Ota, S.; Horie, K.; Hagino, F.; Hashimoto, C.; Date, H.
    Fractionation and some properties of the proteolytically active components of bromelains in the stem and the fruit of the pineapple plant (1972), J. Biochem., 71, 817-830.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.32 3.1
-
 benzoyl-L-Arg amide enzyme form I-1 Ananas comosus
3.4.22.32 6
-
 benzoyl-L-Arg amide enzyme form II-2 Ananas comosus
3.4.22.32 6.6
-
 benzoyl-L-Arg amide enzyme form II-1 Ananas comosus
3.4.22.32 9.5
-
 benzoyl-L-Arg amide enzyme form I-2 Ananas comosus
3.4.22.33 2.1
-
 Nalpha-benzoyl-Arg amide fruit bromelain C Ananas comosus
3.4.22.33 4
-
 Nalpha-benzoyl-Arg amide fruit bromelain A and B Ananas comosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.32 18000
-
 Ananas comosus, enzyme form I-2, II-1 and II-2, gel filtration Ananas comosus
3.4.22.32 28000
-
 Ananas comosus, enzyme form I-1, gel filtration Ananas comosus
3.4.22.33 18000
-
 Ananas comosus, fruit bromelain A, gel filtration Ananas comosus
3.4.22.33 19000
-
 Ananas comosus, fruit bromelain C, gel filtration Ananas comosus
3.4.22.33 28000
-
 Ananas comosus, fruit bromelain B, gel filtration Ananas comosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.32 Ananas comosus
-
 enzyme forms: I-1, I-2, II-1, II-2
-
3.4.22.33 Ananas comosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.32 enzyme forms: I-1, I-2, II-1, II-2 Ananas comosus
3.4.22.33 fractionation Ananas comosus
3.4.22.33 fruit bromelain A, B and C Ananas comosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.32 stem
-
 Ananas comosus
-
3.4.22.33 fruit
-
 Ananas comosus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.32 Benzoyl-L-Arg amide + H2O
-
 Ananas comosus Benzoyl-L-Arg + NH3
-
 ?
3.4.22.32 casein + H2O
-
 Ananas comosus hydrolyzed casein
-
 ?
3.4.22.32 L-Pyroglutamyl-L-Phe-L-Leu 4-nitroanilide + H2O
-
 Ananas comosus ?
-
 ?
3.4.22.33 casein + H2O
-
 Ananas comosus hydrolyzed casein
-
 ?
3.4.22.33 Nalpha-Benzoyl-Arg amide + H2O
-
 Ananas comosus Nalpha-Benzoyl-Arg + NH3
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.32 0.0039
-
 benzoyl-L-Arg amide enzyme form I-2 Ananas comosus
3.4.22.32 0.0046
-
 benzoyl-L-Arg amide enzyme form I-1 Ananas comosus
3.4.22.32 0.018
-
 benzoyl-L-Arg amide enzyme form II-2 Ananas comosus
3.4.22.32 0.027
-
 benzoyl-L-Arg amide enzyme form II-1 Ananas comosus
3.4.22.32 0.87
-
 L-Pyroglutamyl-L-Phe-L-Leu 4-nitroanilide enzyme form F4 Ananas comosus
3.4.22.33 0.0094
-
 Nalpha-benzoyl-Arg amide fruit bromelain C Ananas comosus
3.4.22.33 0.027
-
 Nalpha-benzoyl-Arg amide fruit bromelain B Ananas comosus
3.4.22.33 0.033
-
 Nalpha-benzoyl-Arg amide fruit bromelain A Ananas comosus