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Literature summary extracted from

  • Murachi, T.
    Bromelain enzymes (1970), Methods Enzymol., 19, 273-284.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.4.22.32 Lyophilization causes 27% loss of activity Ananas comosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.32 additional information not: DFP Ananas comosus
3.4.22.33 Mercurials cysteine restores activity Ananas comosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.32 1.2
-
 benzoyl-L-Arg amide
-
 Ananas comosus
3.4.22.32 32
-
 benzoyl-L-Arg methyl ester
-
 Ananas comosus
3.4.22.32 84
-
 tosyl-L-Lys ethyl ester
-
 Ananas comosus
3.4.22.32 170
-
 benzoyl-L-Arg ethyl ester
-
 Ananas comosus
3.4.22.32 210
-
 Benzoyl-Gly ethyl ester
-
 Ananas comosus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.22.32 Ethanol 20% v/v, 37°C, 20 min, 33% loss of activity Ananas comosus
3.4.22.32 Methanol 25% v/v, 25°C, 20 min, stable Ananas comosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.32 Ananas comosus
-
-
-
3.4.22.33 Ananas comosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.32
-
 Ananas comosus
3.4.22.33
-
 Ananas comosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.32 stem
-
 Ananas comosus
-
3.4.22.33 fruit juice Ananas comosus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.32 additional information
-
 assay methods Ananas comosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.32 acetyl-Gly ethyl ester + H2O
-
 Ananas comosus acetyl-Gly + ethanol
-
 ?
3.4.22.32 Benzoyl-DL-Ala ethyl ester + H2O
-
 Ananas comosus Benzoyl-DL-Ala + ethanol
-
 ?
3.4.22.32 Benzoyl-Gly ethyl ester + H2O
-
 Ananas comosus Benzoyl-Gly + ethanol
-
 ?
3.4.22.32 Benzoyl-L-Arg amide + H2O
-
 Ananas comosus Benzoyl-L-Arg + NH3
-
 ?
3.4.22.32 Benzoyl-L-Arg ethyl ester + H2O
-
 Ananas comosus Benzoyl-L-Arg + ethanol
-
 ?
3.4.22.32 Benzoyl-L-Arg methyl ester + H2O
-
 Ananas comosus Benzoyl-L-Arg + methanol
-
 ?
3.4.22.32 casein + H2O
-
 Ananas comosus hydrolyzed casein
-
 ?
3.4.22.32 Gly ethyl ester + H2O
-
 Ananas comosus Gly + ethanol
-
 ?
3.4.22.32 L-Phe ethyl ester + H2O
-
 Ananas comosus L-Phe + ethanol
-
 ?
3.4.22.32 L-Tyr ethyl ester + H2O
-
 Ananas comosus L-Tyr + ethanol
-
 ?
3.4.22.32 Tosyl-L-Arg methyl ester + H2O
-
 Ananas comosus Tosyl-L-Arg + methanol
-
 ?
3.4.22.32 tosyl-L-Lys ethyl ester + H2O
-
 Ananas comosus Tosyl-L-Lys + ethanol
-
 ?
3.4.22.32 Tosyl-L-Lys methyl ester + H2O
-
 Ananas comosus Tosyl-L-Lys + methanol
-
 ?
3.4.22.33 casein + H2O
-
 Ananas comosus hydrolyzed casein
-
 ?
3.4.22.33 Nalpha-Benzoyl-Arg amide + H2O
-
 Ananas comosus Nalpha-Benzoyl-Arg + NH3
-
 ?
3.4.22.33 Nalpha-Benzoyl-DL-Arg 4-nitroanilide + H2O
-
 Ananas comosus Nalpha-Benzoyl-DL-Arg + 4-nitroaniline
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.32 55
-
 pH 6.1, 20 min, 50% loss of activity Ananas comosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.32 0.0035
-
 benzoyl-L-Arg amide
-
 Ananas comosus
3.4.22.32 0.035
-
 Tosyl-L-Lys methyl ester
-
 Ananas comosus
3.4.22.32 0.11
-
 benzoyl-L-Arg methyl ester
-
 Ananas comosus
3.4.22.32 0.36
-
 Benzoyl-Gly ethyl ester
-
 Ananas comosus
3.4.22.32 0.5
-
 benzoyl-L-Arg ethyl ester
-
 Ananas comosus
3.4.22.32 0.55
-
 acetyl-Gly ethyl ester
-
 Ananas comosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.33 additional information
-
-
 Ananas comosus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.32 4 10 5°C, 24 h, stable Ananas comosus