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Literature summary extracted from

  • Buttle, D.J.; Ritonja, A.; Pearl, L.H.; Turk, V.; Barrett, A.J.
    Selective cleavage of glycyl bonds by papaya proteinase IV (1990), FEBS Lett., 260, 195-197.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.25 0.08
-
 tert-butyloxycarbonyl-Ala-Ala-Ala 4-methylcoumarin 7-amide
-
 Carica papaya
3.4.22.25 0.16
-
 tert-butyloxycarbonyl-Ala-Ala-Gly 4-methylcoumarin 7-amide
-
 Carica papaya
3.4.22.25 5.2
-
 tert-butyloxycarbonyl-Ala-Ala-Gly 4-nitroanilide
-
 Carica papaya

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.25 Carica papaya
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.25 latex
-
 Carica papaya
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.25 tert-Butyloxycarbonyl-Ala-Ala-Ala 4-methylcoumarin 7-amide + H2O
-
 Carica papaya ?
-
 ?
3.4.22.25 tert-Butyloxycarbonyl-Ala-Ala-Gly 4-methylcoumarin 7-amide + H2O
-
 Carica papaya ?
-
 ?
3.4.22.25 tert-Butyloxycarbonyl-Ala-Ala-Gly 4-nitroanilide + H2O
-
 Carica papaya ?
-
 ?
3.4.22.25 Trypsin + H2O 16 cleavage points of which 13 are glycyl bonds Carica papaya Hydrolyzed trypsin
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.25 0.08
-
 tert-butyloxycarbonyl-Ala-Ala-Ala 4-methylcoumarin 7-amide
-
 Carica papaya
3.4.22.25 5
-
 tert-butyloxycarbonyl-Ala-Ala-Gly 4-methylcoumarin 7-amide
-
 Carica papaya
3.4.22.25 22
-
 tert-butyloxycarbonyl-Ala-Ala-Gly 4-nitroanilide
-
 Carica papaya