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Literature summary extracted from

  • Kisselev, L.L.; Favorova, O.O.; Kovaleva, G.K.
    Tryptophanyl-tRNA synthetase from beef pancreas (1979), Methods Enzymol., 54, 234-257.
No PubMed abstract available

General Stability

EC Number General Stability Organism
6.1.1.2 not markedly inactivated by repeated freezing and thawing Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.2 2'-O-methyladenosine
-
Bos taurus
6.1.1.2 3'-O-methyl-ATP ATP-diphosphate exchange, noncompetitive with respect to ATP Bos taurus
6.1.1.2 3'-O-Methyladenosine ATP-diphosphate exchange, noncompetitive with respect to ATP Bos taurus
6.1.1.2 4,5,6,7-Tetrafluorotryptophan
-
Bos taurus
6.1.1.2 5,7-Difluorotryptophan
-
Bos taurus
6.1.1.2 5-hydroxytryptophan ATP-diphosphate exchange, weak Bos taurus
6.1.1.2 5-Methyltryptophan ATP-diphosphate exchange, weak Bos taurus
6.1.1.2 6-methyltryptophan ATP-diphosphate exchange, weak Bos taurus
6.1.1.2 9-(2',3'-Dihydroxypropyl)adenine S-isomer Bos taurus
6.1.1.2 9-(2'-Hydroxyethyl)adenine
-
Bos taurus
6.1.1.2 9-(2'-Hydroxyethyl)adenine triphosphate
-
Bos taurus
6.1.1.2 9-(3'-Hydroxypropyl)adenine
-
Bos taurus
6.1.1.2 9-(3'-Hydroxypropyl)adenine triphosphate
-
Bos taurus
6.1.1.2 9-(4'-Hydroxybutyl)adenine
-
Bos taurus
6.1.1.2 9-(4'-Hydroxybutyl)adenine triphosphate
-
Bos taurus
6.1.1.2 adenine
-
Bos taurus
6.1.1.2 adenosine
-
Bos taurus
6.1.1.2 Adenosine-5'-(beta-bromoethane phosphonate)
-
Bos taurus
6.1.1.2 Adenosine-5'-(beta-bromoethanediphosphonate)
-
Bos taurus
6.1.1.2 Adenosine-5'-(beta-chloroethyl phosphate)
-
Bos taurus
6.1.1.2 Adenosine-5'-chloromethylphosphonate ATP-diphosphate exchange, reversible, incompetitive Bos taurus
6.1.1.2 alpha,beta-methylene-ATP
-
Bos taurus
6.1.1.2 beta-Indolyl propionic acid
-
Bos taurus
6.1.1.2 beta-indolylacetic acid
-
Bos taurus
6.1.1.2 beta-Indolylpyruvic acid
-
Bos taurus
6.1.1.2 beta-Methyl-DL-tryptophan ATP-diphosphate exchange Bos taurus
6.1.1.2 D-tryptophan
-
Bos taurus
6.1.1.2 diethyl dicarbonate substrates protect, completely reactivated by hydroxylamine Bos taurus
6.1.1.2 DL-Tryptophanyl hydroxamate ATP-diphosphate exchange Bos taurus
6.1.1.2 gamma-(n-Azidoanilide)-ATP
-
Bos taurus
6.1.1.2 Mixed anhydride of ADP and mesithylenecarboxylic acid
-
Bos taurus
6.1.1.2 Mixed anhydride of AMP and mesithylenecarboxylic acid
-
Bos taurus
6.1.1.2 N-acetyl-L-tryptophan
-
Bos taurus
6.1.1.2 N-Formyl-L-tryptophan
-
Bos taurus
6.1.1.2 NEM
-
Bos taurus
6.1.1.2 tryptamine
-
Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.2 0.00012
-
tRNATrp aminoacylation Bos taurus
6.1.1.2 0.5
-
ATP diphosphate, tryptophan-dependent ATP-diphosphate exchange Bos taurus
6.1.1.2 2
-
ATP tryptophan-dependent ATP-diphosphate exchange Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.2 60000
-
2 * 60000, SDS-PAGE Bos taurus
6.1.1.2 120000
-
gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.2 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.2
-
Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.1.1.2 pancreas
-
Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.2 1.45
-
-
Bos taurus

Storage Stability

EC Number Storage Stability Organism
6.1.1.2 -10°C, in 30% ethylene glycol, stable for at least 6 months Bos taurus
6.1.1.2 -70°C, stable for at least 6 months Bos taurus
6.1.1.2 4°C, enzyme concentration 0.5-4 mg/ml in 10 mM TrisHCl buffer, pH 7.5, 0.1 mM EDTA, 1 mM DTT, without tryptophan, stable for 1 week Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.2 ATP + L-tryptophan + tRNATrp does not activate any natural amino acid except L-tryptophan Bos taurus AMP + diphosphate + L-tryptophan-tRNATrp
-
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Subunits

EC Number Subunits Comment Organism
6.1.1.2 dimer 2 * 60000, SDS-PAGE Bos taurus