Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Woo, K.M.; Chung, W.J.; Ha, D.B.; Goldberg, A.L.; Chung, C.H.
    Protease Ti from Escherichia coli requires ATP hydrolysis for protein breakdown but not for hydrolysis of small peptides (1989), J. Biol. Chem., 264, 2088-2091.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.92 diisopropyl fluorophosphate blocks similarly the hydrolysis of both protein and peptide substrates Escherichia coli
3.4.21.92 NEM inactivates ATPase, no inhibition of peptide hydrolysis Escherichia coli
3.4.21.92 Succinyl-Leu-Tyr 4-methylcoumarin 7-amide at high concentrations complete inhibition of casein breakdown Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.92 1.3
-
 N-Succinyl-Leu-Tyr 4-methylcoumarin 7-amide
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.92 Escherichia coli
-
 RGC125 (lon-)
-
3.4.21.92 Escherichia coli RGC125 (lon-)
-
 RGC125 (lon-)
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.92 N-succinyl-Leu-Tyr 4-methylcoumarin 7-amide + H2O
-
 Escherichia coli N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
 ?
3.4.21.92 N-succinyl-Leu-Tyr 4-methylcoumarin 7-amide + H2O
-
 Escherichia coli RGC125 (lon-) N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
 ?
3.4.21.92 Succinyl-Ala-Ala-Phe 4-methylcoumarin 7-amide + H2O ClpP subunit alone Escherichia coli Succinyl-Ala-Ala + Phe 4-methylcoumarin 7-amide
-
 ?
3.4.21.92 Succinyl-Ala-Ala-Phe 4-methylcoumarin 7-amide + H2O ClpP subunit alone Escherichia coli RGC125 (lon-) Succinyl-Ala-Ala + Phe 4-methylcoumarin 7-amide
-
 ?
3.4.21.92 Succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide + H2O ClpP subunit alone Escherichia coli Succinyl-Leu + Leu + Val-Tyr 4-methylcoumarin 7-amide
-
 ?
3.4.21.92 Succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide + H2O ClpP subunit alone Escherichia coli RGC125 (lon-) Succinyl-Leu + Leu + Val-Tyr 4-methylcoumarin 7-amide
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
3.4.21.92 ATP ATP hydrolysis is required for protein breakdown but not for hydrolysis of small peptides Escherichia coli