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Literature summary extracted from

  • Hwang, B.J.; Woo, K.M.; Goldberg, A.L.; Chung, C.H.
    Protease Ti, a new ATP-dependent protease in Escherichia coli, contains protein-activated ATPase and proteolytic functions in distinct subunits (1988), J. Biol. Chem., 263, 8727-8734.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.21.92 Repeated freezing and thawing leads to loss of activity of subunit ClpA Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.92 diisopropyl fluorophosphate inactivates ClpP Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.92 0.21
-
 ATP for hydrolysis of both casein and ATP Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.92 Ca2+ poor substitute for Mg2+ Escherichia coli
3.4.21.92 Mg2+ optimal concentration: 5.1 mM Escherichia coli
3.4.21.92 Mn2+ can partially substitute for Mg2+ Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.92 23000
-
 x * 80000 (ClpA, SDS-PAGE, behaves as a dimer of MW 140000 Da on gel filtration) + x * 23000 (ClpP, SDS-PAGE, behaves as a complex of 10-12 subunits, MW 260000 Da) Escherichia coli
3.4.21.92 80000
-
 x * 80000 (ClpA, SDS-PAGE, behaves as a dimer of MW 140000 Da on gel filtration) + x * 23000 (ClpP, SDS-PAGE, behaves as a complex of 10-12 subunits, MW 260000 Da) Escherichia coli
3.4.21.92 340000
-
 E. coli Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.92 Escherichia coli
-
 RGC125 (lon-)
-
3.4.21.92 Escherichia coli RGC125 (lon-)
-
 RGC125 (lon-)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.92 ClpA Escherichia coli
3.4.21.92 ClpP Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.92 additional information
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.4.21.92 -30°C, subunit ClpA, 1 month, 50% loss of activity Escherichia coli
3.4.21.92 -30°C, subunit ClpA, 20 mM Tris-HCl, pH 7.8, 5 mM MgCl2, 2 mM DTT, 0.5 mM EDTA, 20% v/v glycerol, 1 month, 50% loss of activity, Escherichia coli
3.4.21.92 4°C, 20 mM Tris-HCl, pH 7.8, 5 mM MgCl2, 0.5 mM EDTA, 10% glycerol, subunit ClpA stable for several months Escherichia coli
3.4.21.92 4°C, 20 mM Tris-HCl, pH 7.8, 5 mM MgCl2, 2 mM DTT, 0.5 mM EDTA, 20% v/v glycerol, 80% loss of activity after 1 week, subunit ClpA Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.92 casein + H2O
-
 Escherichia coli small peptides derived from casein
-
 ?
3.4.21.92 casein + H2O
-
 Escherichia coli RGC125 (lon-) small peptides derived from casein
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.92 ? x * 80000 (ClpA, SDS-PAGE, behaves as a dimer of MW 140000 Da on gel filtration) + x * 23000 (ClpP, SDS-PAGE, behaves as a complex of 10-12 subunits, MW 260000 Da) Escherichia coli
3.4.21.92 More enzyme consists of two components: ClpP and ClpA or ClpX Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.92 65
-
 10 min, 20 mM Tris-HCl, pH 7.8, 5 mM MgCl2, 0.5 mM EDTA, 10% glycerol, little or no loss of activity Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.92 additional information
-
 7.0 (ClpA) Escherichia coli
3.4.21.92 additional information
-
 8.2 (ClpP) Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
3.4.21.92 ATP proteolytic activity Escherichia coli
3.4.21.92 dATP proteolytic activity Escherichia coli