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Literature summary extracted from
- Muconolactone isomerase of the 3-oxoadipate pathway catalyzes dechlorination of 5-chloro-substituted muconolactones (1996), Eur. J. Biochem., 237, 350-356.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.3.4 | (4S,5S)-(+/-)-5-chloro-3-methylmuconolactone | isomerization of (4R,5R)-(+/-)-5-chloro-3-methylmuconolactone | Cupriavidus necator |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.3.4 | 0.66 | - |
(4R,5R/4S,5S)-(+/-)-5-chloro-3-methylmuconolactone | - |
Cupriavidus necator | |
| 5.3.3.4 | 6 | - |
(4S)-(+)-muconolactone | - |
Cupriavidus necator | |
| 5.3.3.4 | 10.3 | - |
(4R,5S)-(+)-5-Chloromuconolactone | - |
Cupriavidus necator | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.4 | 10100 | - |
10 * 10100, SDS-PAGE | Cupriavidus necator |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.3.4 | (4S)-Muconolactone | Cupriavidus necator | - |
? | - |
? | |
| 5.3.3.4 | (4S)-Muconolactone | Cupriavidus necator JMP 134-1 | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.3.4 | Cupriavidus necator | - |
- |
- |
| 5.3.3.4 | Cupriavidus necator | - |
basonym Alcaligenes eutrophus | - |
| 5.3.3.4 | Cupriavidus necator JMP 134-1 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.3.4 | - |
Cupriavidus necator |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.3.3.4 | culture condition:benzoate-grown cell | - |
Cupriavidus necator | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 5.3.3.4 | 4°C, pH 7.5, 100 mM potassium sodium phosphate, 3 months stable | Cupriavidus necator |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.3.4 | (4R,5R)-(+/-)-5-chloro-3-methylmuconolactone | - |
Cupriavidus necator | 2-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid | - |
? | |
| 5.3.3.4 | (4R,5R)-(+/-)-5-chloro-3-methylmuconolactone | - |
Cupriavidus necator JMP 134-1 | 2-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid | - |
? | |
| 5.3.3.4 | (4R,5R/4S,5S)-(+/-)-5-chloro-3-methylmuconolactone | - |
Cupriavidus necator | 2-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid | - |
? | |
| 5.3.3.4 | (4R,5S)-(+)-5-chloromuconolactone | - |
Cupriavidus necator | (2S)-chloro(5-oxo-4,5-dihydrofuran-2-yl)acetic acid | - |
? | |
| 5.3.3.4 | (4R,5S)-(+)-5-chloromuconolactone | - |
Cupriavidus necator JMP 134-1 | (2S)-chloro(5-oxo-4,5-dihydrofuran-2-yl)acetic acid | - |
? | |
| 5.3.3.4 | (4R,5S)-(+/-)-5-chloro-3-methylmuconolactone | preferred substrate over the natural substrate (4S)-muconolactone | Cupriavidus necator | (2S)-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid | - |
? | |
| 5.3.3.4 | (4R,5S)-(+/-)-5-chloro-3-methylmuconolactone | preferred substrate over the natural substrate (4S)-muconolactone | Cupriavidus necator JMP 134-1 | (2S)-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid | - |
? | |
| 5.3.3.4 | (4S)-Muconolactone | - |
Cupriavidus necator | ? | - |
? | |
| 5.3.3.4 | (4S)-Muconolactone | - |
Cupriavidus necator JMP 134-1 | ? | - |
? | |
| 5.3.3.4 | muconolactone | (4S)-(+)-muconolactone | Cupriavidus necator | (5-oxo-4,5-dihydrofuran-2-yl)acetic acid | - |
? | |
| 5.3.3.4 | muconolactone | (4S)-(+)-muconolactone | Cupriavidus necator JMP 134-1 | (5-oxo-4,5-dihydrofuran-2-yl)acetic acid | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.3.3.4 | decamer | 10 * 10100, SDS-PAGE | Cupriavidus necator |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.4 | 70 | - |
20 min, stable up to | Cupriavidus necator |
| 5.3.3.4 | 90 | - |
20 min, 25% loss of activity | Cupriavidus necator |
| 5.3.3.4 | 100 | - |
- |
Cupriavidus necator |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.3.4 | 42.5 | - |
(4R,5R/4S,5S)-(+/-)-5-chloro-3-methylmuconolactone | - |
Cupriavidus necator | |
| 5.3.3.4 | 683 | - |
(4S)-(+)-muconolactone | - |
Cupriavidus necator | |
| 5.3.3.4 | 5950 | - |
(4R,5S)-(+)-5-Chloromuconolactone | - |
Cupriavidus necator | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.4 | 9 | - |
5R/4R,5S)-(+/-)-5-chloro-3-methylmuconolactone, more active in potassium sodium phosphate buffer than in Tris/HCl or borate buffer | Cupriavidus necator |
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