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Literature summary extracted from

  • Pacaud, M.
    Protease II from Escherichia coli. Substrate specificity and kinetic properties (1978), Eur. J. Biochem., 82, 439-451.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.83 DFP
-
 Escherichia coli
3.4.21.83 tosyl-Leu chloromethyl ketone
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.83 0.23
-
 tosyl-Arg methyl ester
-
 Escherichia coli
3.4.21.83 0.31
-
 N-Benzyloxycarbonyl-Lys benzyl ester
-
 Escherichia coli
3.4.21.83 0.33
-
 N-Benzyloxycarbonyl-Lys benzyl ester
-
 Escherichia coli
3.4.21.83 0.47
-
 tosyl-Lys-methyl ester
-
 Escherichia coli
3.4.21.83 0.5
-
 benzoyl-Arg 4-nitroanilide benzoyl-Arg ethyl ester Escherichia coli
3.4.21.83 0.6
-
 N-benzoyl-Arg amide
-
 Escherichia coli
3.4.21.83 80
-
 acetyl-tyrosine 4-nitroanilide
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.83 cytoplasm
-
 Escherichia coli 5737
-

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.83 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.83 Acetyl-Tyr 4-nitroanilide + H2O
-
 Escherichia coli Acetyl-Tyr + 4-nitroaniline
-
 ?
3.4.21.83 benzoyl-Arg-NH2 + H2O
-
 Escherichia coli ?
-
 ?
3.4.21.83 Insulin B-chain + H2O cleaves the carboxymethylated B-chain of insulin at the Arg22-Gly23 bond, but after prolonged periods of incubation it is also able to cleave the Tyr16-Leu17 bond Escherichia coli ?
-
 ?
3.4.21.83 additional information not: luteinizing hormone releasing factor Escherichia coli ?
-
 ?
3.4.21.83 N-Benzoyl-DL-Arg 4-nitroanilide + H2O
-
 Escherichia coli N-Benzoyl-DL-Arg + 4-nitroaniline
-
 ?
3.4.21.83 N-Benzoyl-L-Arg ethyl ester + H2O
-
 Escherichia coli N-Benzoyl-L-Arg + ethanol
-
 ?
3.4.21.83 N-benzyloxycarbonyl-Lys benzyl ester + H2O
-
 Escherichia coli N-benzyloxycarbonyl-Lys + benzyl alcohol
-
 ?
3.4.21.83 N-carbobenzoxy-L-Lys 4-nitrophenyl ester + H2O
-
 Escherichia coli N-carbobenzoxy-L-Lys + 4-nitrophenol
-
 ?
3.4.21.83 Tosyl-Arg methyl ester + H2O
-
 Escherichia coli Tosyl-Arg + methanol
-
 ?
3.4.21.83 Tosyl-Lys methyl ester + H2O
-
 Escherichia coli Tosyl-Lys + benzyl alcohol
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.83 1.5
-
 Acetyl-Tyr 4-nitroanilide
-
 Escherichia coli
3.4.21.83 3.5
-
 N-benzoyl-arginine amide
-
 Escherichia coli
3.4.21.83 43
-
 N-benzyloxycarbonyl-Lys 4-nitrophenyl ester
-
 Escherichia coli
3.4.21.83 55
-
 Tosyl-Lys methyl ester
-
 Escherichia coli
3.4.21.83 55
-
 N-Benzyloxycarbonyl-Lys benzyl ester
-
 Escherichia coli
3.4.21.83 57
-
 N-benzoyl-arginine amide
-
 Escherichia coli
3.4.21.83 60
-
 tosyl-Arg methyl ester
-
 Escherichia coli
3.4.21.83 197
-
 N-benzoyl-Arg ethyl ester
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.83 additional information
-
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.83 6
-
 quick inactivation below Escherichia coli