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Literature summary extracted from

  • Yoshida, N.; Tsuruyama, S.; Nagata, K.; Hirayama, K.; Noda, K.; Makisumi, S.
    Purification and characterization of an acidic amino acid specific endopeptidase of Streptomyces griseus obtained from a commercial preparation (Pronase) (1988), J. Biochem., 104, 451-456.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.82 DFP
-
 Streptomyces griseus
3.4.21.82 additional information metal chelators; metals including Hg2+; not: EDTA; SH-blocking agents Streptomyces griseus
3.4.21.82 PCMB partial Streptomyces griseus
3.4.21.82 tosylphenylalanine chloromethyl ketone partial Streptomyces griseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.82 20000
-
 Streptomyces griseus, gel filtration Streptomyces griseus
3.4.21.82 22000
-
 1 * 22000, Streptomyces griseus, SDS-PAGE in presence of 2-mercaptoethanol Streptomyces griseus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.82 Streptomyces griseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.82
-
 Streptomyces griseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.82 commercial preparation pronase Streptomyces griseus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.82 137
-
-
 Streptomyces griseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.82 additional information preferentially hydrolyzes peptide bonds on the carbonyl-terminal side of either glutamic acid or aspartic acid Streptomyces griseus ?
-
 ?
3.4.21.82 tert-butoxycarbonyl-Ala-Ala-Pro-Asp 4-nitroanilide + H2O slowly Streptomyces griseus tert-butoxycarbonyl-Ala-Ala-Pro-Asp + 4-nitroaniline
-
 ?
3.4.21.82 tert-butoxycarbonyl-Ala-Ala-Pro-Glu 4-nitroanilide + H2O
-
 Streptomyces griseus tert-butoxycarbonyl-Ala-Ala-Pro-Glu + 4-nitroaniline
-
 ?
3.4.21.82 tert-butoxycarbonyl-Ala-Phe-Pro-Glu 4-nitroanilide + H2O
-
 Streptomyces griseus tert-butoxycarbonyl-Ala-Phe-Pro-Glu + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.82 monomer 1 * 22000, Streptomyces griseus, SDS-PAGE in presence of 2-mercaptoethanol Streptomyces griseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.82 additional information
-
 pI: 8.4 Streptomyces griseus
3.4.21.82 8.8
-
 tert-butoxycarbonyl-Ala-Ala-Pro-Glu 4-nitroanilide Streptomyces griseus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.82 5 9 relatively stable Streptomyces griseus