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Literature summary extracted from
- Conversion of catechol and protocatechuate to beta-ketoadipate (Pseudomonas putida) (1970), Methods Enzymol., 17A, 529-549.
No PubMed abstract available
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 5.3.3.4 | - |
Pseudomonas putida |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.5.1.2 | phosphate | - |
Pseudomonas putida |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.24 | 0.012 | - |
2-(5-oxo-4,5-dihydrofuran-2-yl)acetic acid | - |
Pseudomonas putida | |
| 4.1.1.44 | 0.82 | - |
4-carboxymuconolactone | - |
Pseudomonas putida | |
| 5.3.3.4 | 0.08 | - |
muconolactone | - |
Pseudomonas putida | |
| 5.5.1.1 | 0.1 | - |
cis,cis-muconate | - |
Pseudomonas putida | |
| 5.5.1.2 | 0.075 | - |
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate | - |
Pseudomonas putida | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.5.1.1 | Mg2+ | can partially replace Mg2+ | Pseudomonas putida | |
| 5.5.1.1 | Mn2+ | required | Pseudomonas putida | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.24 | 33000 | - |
gel filtration | Pseudomonas putida |
| 4.1.1.44 | 93000 | - |
- |
Pseudomonas putida |
| 5.3.3.4 | 93000 | - |
- |
Pseudomonas putida |
| 5.5.1.1 | 220000 | - |
- |
Pseudomonas putida |
| 5.5.1.2 | 190000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.24 | (5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O | Pseudomonas putida | the enzyme acts on the product of EC 4.1.1.44 4-carboxymuconolactone decarboxylase | ? | - |
? | |
| 5.3.3.4 | additional information | Pseudomonas putida | enzyme of beta-ketoadipate pathway | ? | - |
? | |
| 5.3.3.4 | additional information | Pseudomonas putida A.3.12. / ATCC 12633 | enzyme of beta-ketoadipate pathway | ? | - |
? | |
| 5.5.1.2 | additional information | Pseudomonas putida | enzyme of the beta-ketoadipate pathway | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.24 | Pseudomonas putida | - |
- |
- |
| 4.1.1.44 | Pseudomonas putida | - |
- |
- |
| 5.3.3.4 | Pseudomonas putida | - |
- |
- |
| 5.5.1.1 | Pseudomonas putida | - |
- |
- |
| 5.5.1.2 | Pseudomonas putida | - |
- |
- |
| 5.5.1.2 | Pseudomonas putida A.3.12. / ATCC 12633 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.24 | 422fold | Pseudomonas putida |
| 4.1.1.44 | - |
Pseudomonas putida |
| 5.3.3.4 | - |
Pseudomonas putida |
| 5.5.1.1 | - |
Pseudomonas putida |
| 5.5.1.2 | - |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.24 | 717 | - |
- |
Pseudomonas putida |
| 5.3.3.4 | additional information | - |
- |
Pseudomonas putida |
| 5.5.1.1 | 87.3 | - |
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.24 | (5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O | 3-oxoadipate enol-lactone | Pseudomonas putida | 3-oxoadipate | - |
? | |
| 3.1.1.24 | (5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O | the enzyme acts on the product of EC 4.1.1.44 4-carboxymuconolactone decarboxylase | Pseudomonas putida | ? | - |
? | |
| 4.1.1.44 | 4-carboxymuconolactone | - |
Pseudomonas putida | 5-oxo-4,5-dihydrofuran-2-acetate + CO2 | beta-ketoadipate enol-lactone | ? | |
| 5.3.3.4 | additional information | enzyme of beta-ketoadipate pathway | Pseudomonas putida | ? | - |
? | |
| 5.3.3.4 | additional information | enzyme of beta-ketoadipate pathway | Pseudomonas putida A.3.12. / ATCC 12633 | ? | - |
? | |
| 5.3.3.4 | muconolactone | - |
Pseudomonas putida | (5-oxo-4,5-dihydrofuran-2-yl)acetic acid | - |
? | |
| 5.3.3.4 | muconolactone | - |
Pseudomonas putida A.3.12. / ATCC 12633 | (5-oxo-4,5-dihydrofuran-2-yl)acetic acid | - |
? | |
| 5.5.1.1 | cis,cis-Muconate | - |
Pseudomonas putida | (+)-Muconolactone | - |
? | |
| 5.5.1.1 | cis,cis-Muconate | - |
Pseudomonas putida A.3.12. / ATCC 12633 | (+)-Muconolactone | - |
? | |
| 5.5.1.1 | cis,trans-Muconate | - |
Pseudomonas putida | ? | - |
? | |
| 5.5.1.1 | cis,trans-Muconate | - |
Pseudomonas putida A.3.12. / ATCC 12633 | ? | - |
? | |
| 5.5.1.1 | additional information | no activity against 2-carboxy-cis,cis-muconate | Pseudomonas putida | ? | - |
? | |
| 5.5.1.1 | additional information | no activity against 2-carboxy-cis,cis-muconate | Pseudomonas putida A.3.12. / ATCC 12633 | ? | - |
? | |
| 5.5.1.2 | 2-Carboxy-2,5-dihydro-5-oxofuran-2-acetate | i.e. beta-carboxy-cis,cis-muconate | Pseudomonas putida | cis-Butadiene-1,2,4-tricarboxylate | i.e. gamma-carboxymuconolactone | ? | |
| 5.5.1.2 | additional information | enzyme of the beta-ketoadipate pathway | Pseudomonas putida | ? | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.5.1.1 | 60 | - |
quite stable at | Pseudomonas putida |
| 5.5.1.2 | 60 | - |
stable | Pseudomonas putida |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.24 | 7.5 | 10 | - |
Pseudomonas putida |
| 4.1.1.44 | 6.5 | 8 | - |
Pseudomonas putida |
| 5.3.3.4 | 7.5 | 9 | - |
Pseudomonas putida |
| 5.5.1.1 | 7 | - |
- |
Pseudomonas putida |
| 5.5.1.2 | 7 | - |
around | Pseudomonas putida |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.5.1.1 | 6 | 8 | about 65% of maximal activity at pH 6 and pH 8 | Pseudomonas putida |
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