Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Jean, F.; Basak, A.; Rondeau, N.; Benjannet, S.; Hendy, G.N.; Seidah, N.G.
    Enzymic characterization of murine and human prohormone convertase-1 (mPC1 and hPC1) expressed in mammalian GH4C1 cells (1993), Biochem. J., 292, 891-900.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.75 antipain
-
 Homo sapiens
3.4.21.75 antipain
-
 Mus musculus
3.4.21.75 Arg-oxime
-
 Homo sapiens
3.4.21.75 Arg-oxime
-
 Mus musculus
3.4.21.75 Ca2+ 100 mM; activation below Homo sapiens
3.4.21.75 Ca2+ 100 mM; activation below Mus musculus
3.4.21.75 CDTA
-
 Homo sapiens
3.4.21.75 CDTA
-
 Mus musculus
3.4.21.75 EDTA
-
 Homo sapiens
3.4.21.75 EDTA
-
 Mus musculus
3.4.21.75 EGTA
-
 Homo sapiens
3.4.21.75 EGTA
-
 Mus musculus
3.4.21.75 leupeptin moderately Homo sapiens
3.4.21.75 leupeptin moderately Mus musculus
3.4.21.75 Lys-Arg chloromethyl ketone
-
 Homo sapiens
3.4.21.75 Lys-Arg chloromethyl ketone
-
 Mus musculus
3.4.21.75 additional information not: pepstatin Homo sapiens
3.4.21.75 additional information not: pepstatin Mus musculus
3.4.21.75 NaCl 600 mM Homo sapiens
3.4.21.75 NaCl 600 mM Mus musculus
3.4.21.75 p-hydroxymercuribenzoate
-
 Homo sapiens
3.4.21.75 p-hydroxymercuribenzoate
-
 Mus musculus
3.4.21.75 phenylmethanesulfonyl fluoride not Homo sapiens
3.4.21.75 phenylmethanesulfonyl fluoride not Mus musculus
3.4.21.75 tosyl-Lys chloromethyl ketone
-
 Homo sapiens
3.4.21.75 tosyl-Lys chloromethyl ketone
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.75 additional information
-
 additional information
-
 Mus musculus
3.4.21.75 additional information
-
 additional information
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.75 Ca2+ required Mus musculus
3.4.21.75 Ca2+ required Homo sapiens
3.4.21.75 Ca2+ 2.46 mM Mus musculus
3.4.21.75 Ca2+ 2.46 mM Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.75 Homo sapiens
-
 rat somatomammotroph cell line, GH4C1 infected with vaccinia virus recombinants of human PC1
-
3.4.21.75 Mus musculus
-
 rat somatomammotroph cell line, GH4C1 infected with vaccinia virus recombinants of murine PC1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.75 partial Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.75 Acetyl-Arg-Ser-Lys-Arg-4-methylcoumarin 7-amide + H2O
-
 Mus musculus ?
-
 ?
3.4.21.75 Acetyl-Arg-Ser-Lys-Arg-4-methylcoumarin 7-amide + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.75 pGlu-Arg-Thr-Lys-Arg-4-methylcoumarin 7-amide + H2O
-
 Mus musculus pGlu-Arg-Thr-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.75 pGlu-Arg-Thr-Lys-Arg-4-methylcoumarin 7-amide + H2O
-
 Homo sapiens pGlu-Arg-Thr-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.75 tert-butoxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumarin 7-amide + H2O
-
 Mus musculus tert-butoxycarbonyl-Arg-Val-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.75 tert-butoxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumarin 7-amide + H2O
-
 Homo sapiens tert-butoxycarbonyl-Arg-Val-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.75 ? x * 80000-85000, mouse, SDS-PAGE Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.75 6
-
 acetyl-Arg-Ser-Lys-Arg-4-methylcoumaryl 7-amide Mus musculus
3.4.21.75 6
-
 acetyl-Arg-Ser-Lys-Arg-4-methylcoumaryl 7-amide Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.75 5 6.5 5: about 35% of activity maximum, 6.5: about 15% of activity maximum, mouse, acetyl-Arg-Ser-Lys-Arg-4-methylcoumarin 7-amide Mus musculus
3.4.21.75 5 6.5 5: about 35% of activity maximum, 6.5: about 15% of activity maximum, mouse, acetyl-Arg-Ser-Lys-Arg-4-methylcoumarin 7-amide Homo sapiens