BRENDA - Enzyme Database

Ancrod, the coagulating enzyme from Malayan pit viper (Agkistrodon rhodostoma) venom

Nolan, C.; Hall, L.S.; Barlow, G.H.; Methods Enzymol. 45, 205-213 (1976)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.21.74
4-Nitrophenyl-4-guanidinobenzoate
-
Calloselasma rhodostoma
3.4.21.74
agmatine
-
Calloselasma rhodostoma
3.4.21.74
Alpha-macroglobulin
-
Calloselasma rhodostoma
3.4.21.74
alpha-N-(4-Nitrobenzyloxycarbonyl)-Arg chloromethyl ketone
-
Calloselasma rhodostoma
3.4.21.74
antithrombin III
-
Calloselasma rhodostoma
3.4.21.74
arginine
-
Calloselasma rhodostoma
3.4.21.74
diisopropyl fluorophosphate
-
Calloselasma rhodostoma
3.4.21.74
guanidinium ions
-
Calloselasma rhodostoma
3.4.21.74
additional information
no inhibition by Nalpha-p-tosyl-L-lysine chloromethyl ketone
Calloselasma rhodostoma
3.4.21.74
PMSF
-
Calloselasma rhodostoma
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.4.21.74
soluble
-
Calloselasma rhodostoma
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.21.74
additional information
-
amino acid composition
Calloselasma rhodostoma
3.4.21.74
35400
-
Agkistrodon rhodostoma, sedimentation equilibrium
Calloselasma rhodostoma
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.4.21.74
Calloselasma rhodostoma
-
Malayan pit viper, formerly EC 3.4.21.28
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.4.21.74
glycoprotein
high carbohydrate content
Calloselasma rhodostoma
Purification (Commentary)
EC Number
Commentary
Organism
3.4.21.74
affinity chromatography, several forms, separable by electrophoresis and isoelectric focusing
Calloselasma rhodostoma
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.21.74
venom
-
Calloselasma rhodostoma
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.4.21.74
33.7
-
tosyl-L-Arg methyl ester
Calloselasma rhodostoma
Storage Stability
EC Number
Storage Stability
Organism
3.4.21.74
5C, pH 5-6.8, several months
Calloselasma rhodostoma
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.4.21.74
Fibrinogen + H2O
splits fibrinopeptide A from Aalpha-chains by hydrolysis of Arg-Gly bond, but does not split this bond in Bbeta-chain
29684
Calloselasma rhodostoma
?
-
-
-
-
3.4.21.74
Fibrinogen + H2O
or gamma-chain
29684
Calloselasma rhodostoma
?
-
-
-
-
3.4.21.74
additional information
no activation of human factor VIII
29684
Calloselasma rhodostoma
?
-
-
-
-
3.4.21.74
additional information
Ancrod has a high degree of specificity for Arg esters and peptide bonds involving carboxyl group of Arg residues
29684
Calloselasma rhodostoma
?
-
-
-
-
3.4.21.74
additional information
no hydrolysis of native casein, denatured hemoglobin, Nalpha-benzoyl-Arg ethyl ester
29684
Calloselasma rhodostoma
?
-
-
-
-
3.4.21.74
Nalpha-Benzoyl-L-Arg cyclohexyl ester + H2O
-
29684
Calloselasma rhodostoma
Nalpha-Benzoyl-L-Arg + cyclohexanol
-
-
-
-
3.4.21.74
Nalpha-Benzoyl-L-Arg ethyl ester + H2O
-
29684
Calloselasma rhodostoma
Nalpha-Benzoyl-L-Arg + ethanol
-
-
-
-
3.4.21.74
Nalpha-Benzoyl-L-Arg methyl ester + H2O
-
29684
Calloselasma rhodostoma
Nalpha-Benzoyl-L-Arg + methanol
-
-
-
-
3.4.21.74
Tosyl-L-arginine methyl ester + H2O
-
29684
Calloselasma rhodostoma
Tosyl-L-arginine + methanol
-
-
-
-
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.4.21.74
37
-
assay at
Calloselasma rhodostoma
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.4.21.74
5
6.8
several months stable at 5C
Calloselasma rhodostoma
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.21.74
4-Nitrophenyl-4-guanidinobenzoate
-
Calloselasma rhodostoma
3.4.21.74
agmatine
-
Calloselasma rhodostoma
3.4.21.74
Alpha-macroglobulin
-
Calloselasma rhodostoma
3.4.21.74
alpha-N-(4-Nitrobenzyloxycarbonyl)-Arg chloromethyl ketone
-
Calloselasma rhodostoma
3.4.21.74
antithrombin III
-
Calloselasma rhodostoma
3.4.21.74
arginine
-
Calloselasma rhodostoma
3.4.21.74
diisopropyl fluorophosphate
-
Calloselasma rhodostoma
3.4.21.74
guanidinium ions
-
Calloselasma rhodostoma
3.4.21.74
additional information
no inhibition by Nalpha-p-tosyl-L-lysine chloromethyl ketone
Calloselasma rhodostoma
3.4.21.74
PMSF
-
Calloselasma rhodostoma
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.4.21.74
soluble
-
Calloselasma rhodostoma
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.21.74
additional information
-
amino acid composition
Calloselasma rhodostoma
3.4.21.74
35400
-
Agkistrodon rhodostoma, sedimentation equilibrium
Calloselasma rhodostoma
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.4.21.74
glycoprotein
high carbohydrate content
Calloselasma rhodostoma
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.21.74
affinity chromatography, several forms, separable by electrophoresis and isoelectric focusing
Calloselasma rhodostoma
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.21.74
venom
-
Calloselasma rhodostoma
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.4.21.74
33.7
-
tosyl-L-Arg methyl ester
Calloselasma rhodostoma
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.4.21.74
5C, pH 5-6.8, several months
Calloselasma rhodostoma
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.4.21.74
Fibrinogen + H2O
splits fibrinopeptide A from Aalpha-chains by hydrolysis of Arg-Gly bond, but does not split this bond in Bbeta-chain
29684
Calloselasma rhodostoma
?
-
-
-
-
3.4.21.74
Fibrinogen + H2O
or gamma-chain
29684
Calloselasma rhodostoma
?
-
-
-
-
3.4.21.74
additional information
no activation of human factor VIII
29684
Calloselasma rhodostoma
?
-
-
-
-
3.4.21.74
additional information
Ancrod has a high degree of specificity for Arg esters and peptide bonds involving carboxyl group of Arg residues
29684
Calloselasma rhodostoma
?
-
-
-
-
3.4.21.74
additional information
no hydrolysis of native casein, denatured hemoglobin, Nalpha-benzoyl-Arg ethyl ester
29684
Calloselasma rhodostoma
?
-
-
-
-
3.4.21.74
Nalpha-Benzoyl-L-Arg cyclohexyl ester + H2O
-
29684
Calloselasma rhodostoma
Nalpha-Benzoyl-L-Arg + cyclohexanol
-
-
-
-
3.4.21.74
Nalpha-Benzoyl-L-Arg ethyl ester + H2O
-
29684
Calloselasma rhodostoma
Nalpha-Benzoyl-L-Arg + ethanol
-
-
-
-
3.4.21.74
Nalpha-Benzoyl-L-Arg methyl ester + H2O
-
29684
Calloselasma rhodostoma
Nalpha-Benzoyl-L-Arg + methanol
-
-
-
-
3.4.21.74
Tosyl-L-arginine methyl ester + H2O
-
29684
Calloselasma rhodostoma
Tosyl-L-arginine + methanol
-
-
-
-
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.4.21.74
37
-
assay at
Calloselasma rhodostoma
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.4.21.74
5
6.8
several months stable at 5C
Calloselasma rhodostoma