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Literature summary extracted from

  • Nolan, C.; Hall, L.S.; Barlow, G.H.
    Ancrod, the coagulating enzyme from Malayan pit viper (Agkistrodon rhodostoma) venom (1976), Methods Enzymol., 45, 205-213.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.74 4-Nitrophenyl-4-guanidinobenzoate
-
 Calloselasma rhodostoma
3.4.21.74 agmatine
-
 Calloselasma rhodostoma
3.4.21.74 Alpha-macroglobulin
-
 Calloselasma rhodostoma
3.4.21.74 alpha-N-(4-Nitrobenzyloxycarbonyl)-Arg chloromethyl ketone
-
 Calloselasma rhodostoma
3.4.21.74 antithrombin III
-
 Calloselasma rhodostoma
3.4.21.74 arginine
-
 Calloselasma rhodostoma
3.4.21.74 diisopropyl fluorophosphate
-
 Calloselasma rhodostoma
3.4.21.74 guanidinium ions
-
 Calloselasma rhodostoma
3.4.21.74 additional information no inhibition by Nalpha-p-tosyl-L-lysine chloromethyl ketone Calloselasma rhodostoma
3.4.21.74 PMSF
-
 Calloselasma rhodostoma

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.74 soluble
-
 Calloselasma rhodostoma
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.74 additional information
-
 amino acid composition Calloselasma rhodostoma
3.4.21.74 35400
-
 Agkistrodon rhodostoma, sedimentation equilibrium Calloselasma rhodostoma

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.74 Calloselasma rhodostoma
-
 Malayan pit viper, formerly EC 3.4.21.28
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.74 glycoprotein high carbohydrate content Calloselasma rhodostoma

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.74 affinity chromatography, several forms, separable by electrophoresis and isoelectric focusing Calloselasma rhodostoma

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.74 venom
-
 Calloselasma rhodostoma
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.74 33.7
-
 tosyl-L-Arg methyl ester Calloselasma rhodostoma

Storage Stability

EC Number Storage Stability Organism
3.4.21.74 5°C, pH 5-6.8, several months Calloselasma rhodostoma

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.74 Fibrinogen + H2O splits fibrinopeptide A from Aalpha-chains by hydrolysis of Arg-Gly bond, but does not split this bond in Bbeta-chain Calloselasma rhodostoma ?
-
 ?
3.4.21.74 Fibrinogen + H2O or gamma-chain Calloselasma rhodostoma ?
-
 ?
3.4.21.74 additional information no activation of human factor VIII Calloselasma rhodostoma ?
-
 ?
3.4.21.74 additional information Ancrod has a high degree of specificity for Arg esters and peptide bonds involving carboxyl group of Arg residues Calloselasma rhodostoma ?
-
 ?
3.4.21.74 additional information no hydrolysis of native casein, denatured hemoglobin, Nalpha-benzoyl-Arg ethyl ester Calloselasma rhodostoma ?
-
 ?
3.4.21.74 Nalpha-Benzoyl-L-Arg cyclohexyl ester + H2O
-
 Calloselasma rhodostoma Nalpha-Benzoyl-L-Arg + cyclohexanol
-
 ?
3.4.21.74 Nalpha-Benzoyl-L-Arg ethyl ester + H2O
-
 Calloselasma rhodostoma Nalpha-Benzoyl-L-Arg + ethanol
-
 ?
3.4.21.74 Nalpha-Benzoyl-L-Arg methyl ester + H2O
-
 Calloselasma rhodostoma Nalpha-Benzoyl-L-Arg + methanol
-
 ?
3.4.21.74 Tosyl-L-arginine methyl ester + H2O
-
 Calloselasma rhodostoma Tosyl-L-arginine + methanol
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.74 37
-
 assay at Calloselasma rhodostoma

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.74 5 6.8 several months stable at 5°C Calloselasma rhodostoma