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Literature summary extracted from

  • Simmons, G.; Bundalian, M.; Theodor, I.; Martinoli, J.; Pirkle, H.
    Action of crotalase, an enzyme with thrombin-like and kallikrein-like specificities, on tripeptide nitroanilide derivatives (1985), Thromb. Res., 40, 555-561.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.74 H-D-Nle-cyclohexylalanyl-Arg 4-nitroanilide substrate inhibition, 1.24 mM and above Crotalus adamanteus
3.4.21.74 H-D-Phenylglycyl-Phe-Arg 4-nitroanilide substrate inhibition, 0.31 mM and above Crotalus adamanteus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.74 0.025
-
 carbobenzoxyl-Glu-(alpha-tert-butoxy)-Gly-Arg-4-nitroanilide
-
 Crotalus adamanteus
3.4.21.74 0.058
-
 H-D-Pro-hexahydrotyrosyl-Arg 4-nitroanilide
-
 Crotalus adamanteus
3.4.21.74 0.18
-
 D-Phe-L-pipecoyl-L-Arg 4-nitroanilide H-D-cyclohexylglycyl-L-alpha-aminobutyryl-Arg 4-nitroanilide Crotalus adamanteus
3.4.21.74 0.27
-
 H-D-Nle-cyclohexylalanyl-Arg 4-nitroanilide H-D-phenylglycyl-Phe-Arg 4-nitroanilide Crotalus adamanteus
3.4.21.74 0.3
-
 Tosyl-Gly-Pro-Arg 4-nitroanilide
-
 Crotalus adamanteus
3.4.21.74 0.39
-
 benzoyl-Pro-Phe-Arg 4-nitroanilide
-
 Crotalus adamanteus
3.4.21.74 0.45
-
 H-D-Hexahydrotyrosyl-Ala-Arg 4-nitroanilide
-
 Crotalus adamanteus
3.4.21.74 1.4
-
 tosyl-Gly-Pro-Lys 4-nitroanilide
-
 Crotalus adamanteus
3.4.21.74 2.7
-
 H-D-Nle-hexahydrotyrosyl-Lys 4-nitroanilide
-
 Crotalus adamanteus
3.4.21.74 3.3
-
 H-D-Aminobutyric acid-cyclohexylalanyl-Lys 4-nitroanilide methylsulfonyl-Leu-Gly-Arg 4-nitroanilide Crotalus adamanteus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.74 Crotalus adamanteus
-
 Eastern diamondback rattlesnake, formerly EC 3.4.21.30
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.74 venom
-
 Crotalus adamanteus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.74 Benzoyl-Pro-Phe-Arg 4-nitroanilide + H2O
-
 Crotalus adamanteus Benzoyl-Pro-Phe-Arg + 4-nitroaniline
-
 ?
3.4.21.74 benzyloxycarbonyl-Phe 4-nitrophenyl ester + H2O
-
 Crotalus adamanteus benzyloxycarbonyl-Phe + 4-nitrophenol
-
 ?
3.4.21.74 Carbobenzoxyl-Glu-(alpha-t-butoxy)-Gly-Arg 4-nitroanilide + H2O
-
 Crotalus adamanteus Carbobenzoxyl-Glu-(alpha-t-butoxy)-Gly-Arg + 4-nitroaniline
-
 ?
3.4.21.74 carbobenzoxyl-Glu-(alpha-tert-butoxy)-Gly-Arg-4-nitroanilide + H2O
-
 Crotalus adamanteus carbobenzoxyl-Glu-(alpha-tert-butoxy)-Gly-Arg + 4-nitroaniline
-
 ?
3.4.21.74 D-aminobutyric acid-cyclohexylalanyl-Lys 4-nitroanilide + H2O
-
 Crotalus adamanteus D-aminobutyric acid-cyclohexylalanyl-Lys + 4-nitroaniline
-
 ?
3.4.21.74 D-Phe-L-pipecoyl-L-Arg 4-nitroanilide + H2O
-
 Crotalus adamanteus ?
-
 ?
3.4.21.74 H-D-Cyclohexylglycyl-aminobutyric acid-cyclohexylalanyl-Lys 4-nitroanilide + H2O
-
 Crotalus adamanteus H-D-Cyclohexylglycyl-aminobutyric acid-cyclohexylalanyl-Lys + 4-nitroaniline
-
 ?
3.4.21.74 H-D-Cyclohexylglycyl-L-2-aminobutyryl-Arg 4-nitroanilide + H2O
-
 Crotalus adamanteus H-D-Cyclohexylglycyl-L-alpha-aminobutyryl-L-arginine + 4-nitroaniline
-
 ?
3.4.21.74 H-D-Hexahydrotyrosyl-Ala-Arg 4-nitroanilide + H2O
-
 Crotalus adamanteus H-D-Hexahydrotyrosyl-Ala-Arg + 4-nitroaniline
-
 ?
3.4.21.74 H-D-Nle-cyclohexylalanyl-Arg 4-nitroanilide + H2O
-
 Crotalus adamanteus H-D-Nle-cyclohexylalanyl-L-arginine + 4-nitroaniline
-
 ?
3.4.21.74 H-D-Nle-hexahydrotyrosyl-Lys 4-nitroanilide + H2O
-
 Crotalus adamanteus H-D-Nle-hexahydrotyrosyl-Lys + 4-nitroaniline
-
 ?
3.4.21.74 H-D-Phenylglycyl-Phe-Arg 4-nitroanilide + H2O
-
 Crotalus adamanteus H-D-Phenylglycyl-Phe-Arg + 4-nitroaniline
-
 ?
3.4.21.74 H-D-Pro-hexahydrotyrosyl-Arg 4-nitroanilide + H2O
-
 Crotalus adamanteus H-D-Pro-hexahydrotyrosyl-L-arginine + 4-nitroaniline
-
 ?
3.4.21.74 Methylsulfonyl-Leu-Gly-Arg 4-nitroanilide + H2O
-
 Crotalus adamanteus Methylsulfonyl-Leu-Gly-Arg + 4-nitroaniline
-
 ?
3.4.21.74 additional information no plasmin-like activity Crotalus adamanteus ?
-
 ?
3.4.21.74 additional information specificity compared to that of thrombin and kallikrein Crotalus adamanteus ?
-
 ?
3.4.21.74 additional information hydrolyzes Arg-bonds better than Lys-bonds Crotalus adamanteus ?
-
 ?
3.4.21.74 Tosyl-Gly-Pro-Arg 4-nitroanilide + H2O
-
 Crotalus adamanteus Tosyl-Gly-Pro-Arg + 4-nitroaniline
-
 ?
3.4.21.74 Tosyl-Gly-Pro-Lys 4-nitroanilide + H2O
-
 Crotalus adamanteus Tosyl-Gly-Pro-Lys + 4-nitroaniline
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.74 27
-
 assay at Crotalus adamanteus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.74 0.16
-
 carbobenzoxyl-Glu-(alpha-tert-butoxy)-Gly-Arg-4-nitroanilide
-
 Crotalus adamanteus
3.4.21.74 0.35
-
 benzoyl-Pro-Phe-Arg 4-nitroanilide
-
 Crotalus adamanteus
3.4.21.74 0.68
-
 H-D-Cyclohexylglycyl-L-2-aminobutyryl-Arg 4-nitroanilide
-
 Crotalus adamanteus
3.4.21.74 0.7
-
 tosyl-Gly-Pro-Lys 4-nitroanilide
-
 Crotalus adamanteus
3.4.21.74 0.95
-
 H-D-Hexahydrotyrosyl-Ala-Arg 4-nitroanilide
-
 Crotalus adamanteus
3.4.21.74 1.3
-
 Methylsulfonyl-Leu-Gly-Arg 4-nitroanilide
-
 Crotalus adamanteus
3.4.21.74 1.4
-
 H-D-Aminobutyric acid-cyclohexylalanyl-Lys 4-nitroanilide
-
 Crotalus adamanteus
3.4.21.74 3.5
-
 D-phenylglycyl-Phe-Arg 4-nitroanilide
-
 Crotalus adamanteus
3.4.21.74 4.9
-
 H-D-Pro-hexahydrotyrosyl-Arg 4-nitroanilide
-
 Crotalus adamanteus
3.4.21.74 6.9
-
 H-D-Nle-hexahydrotyrosyl-Lys 4-nitroanilide
-
 Crotalus adamanteus
3.4.21.74 11.5
-
 Tosyl-Gly-Pro-Arg 4-nitroanilide
-
 Crotalus adamanteus
3.4.21.74 61
-
 H-D-Nle-cyclohexylalanyl-Arg 4-nitroanilide
-
 Crotalus adamanteus