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Literature summary extracted from

  • Chao, J.
    Purification and characterization of rat urinary esterase A, a plasminogen activator (1983), J. Biol. Chem., 258, 4434-4439.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.21.73 Unstable to dilution Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.73 alpha-1-antitrypsin
-
 Rattus norvegicus
3.4.21.73 antipain
-
 Rattus norvegicus
3.4.21.73 Aprotinin
-
 Rattus norvegicus
3.4.21.73 benzamidine
-
 Rattus norvegicus
3.4.21.73 D-Phe-Phe-ArgCH2Cl
-
 Rattus norvegicus
3.4.21.73 leupeptin
-
 Rattus norvegicus
3.4.21.73 Lima bean trypsin inhibitor
-
 Rattus norvegicus
3.4.21.73 Pentamidine
-
 Rattus norvegicus
3.4.21.73 phenylmethanesulfonyl fluoride
-
 Rattus norvegicus
3.4.21.73 Soybean trypsin inhibitor
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.73 0.053
-
 N-benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-trifluoromethylcoumarin
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.73 8550
-
 x * 15800 + x * 14200 + x * 8850 + x * 8550, SDS-PAGE under reducing conditions, x * 24500 + x * 23000, SDS-PAGE under nonreducing conditions Rattus norvegicus
3.4.21.73 8850
-
 x * 15800 + x * 14200 + x * 8850 + x * 8550, SDS-PAGE under reducing conditions, x * 24500 + x * 23000, SDS-PAGE under nonreducing conditions Rattus norvegicus
3.4.21.73 14200
-
 x * 15800 + x * 14200 + x * 8850 + x * 8550, SDS-PAGE under reducing conditions, x * 24500 + x * 23000, SDS-PAGE under nonreducing conditions Rattus norvegicus
3.4.21.73 15800
-
 x * 15800 + x * 14200 + x * 8850 + x * 8550, SDS-PAGE under reducing conditions, x * 24500 + x * 23000, SDS-PAGE under nonreducing conditions Rattus norvegicus
3.4.21.73 23000
-
 x * 15800 + x * 14200 + x * 8850 + x * 8550, SDS-PAGE under reducing conditions, x * 24500 + x * 23000, SDS-PAGE under nonreducing conditions Rattus norvegicus
3.4.21.73 24500
-
 x * 15800 + x * 14200 + x * 8850 + x * 8550, SDS-PAGE under reducing conditions, x * 24500 + x * 23000, SDS-PAGE under nonreducing conditions Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.73 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.73
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.73 urine
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.73 additional information
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.4.21.73 -20°C, 0.01 M sodium phosphate buffer, pH 7.0, 0.2 mg/ml enzyme concentration, stable Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.73 kininogen + H2O
-
 Rattus norvegicus kinin + ?
-
 ?
3.4.21.73 additional information lyses fibrin clots containing plasminogen but not plasminogen-free fibrin Rattus norvegicus ?
-
 ?
3.4.21.73 N-benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-trifluoromethylcoumarin + H2O
-
 Rattus norvegicus N-benzyloxycarbonyl-Gly-Gly-Arg + 7-amino-4-trifluoromethylcoumarin
-
 ?
3.4.21.73 plasminogen + H2O
-
 Rattus norvegicus plasmin + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.73 ? x * 15800 + x * 14200 + x * 8850 + x * 8550, SDS-PAGE under reducing conditions, x * 24500 + x * 23000, SDS-PAGE under nonreducing conditions Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.4.21.73 Urinary esterase A
-
 Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.73 55
-
 30-60 min, 72-82% loss of activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.73 8.5 9
-
 Rattus norvegicus