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Literature summary extracted from

  • Scherf, U.; Söhling, B.; Gottschalk, G.; Linder, D.; Buckel, W.
    Succinate-ethanol fermentation in Clostridium kluyveri: purification, characterisation of 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA DELTA3-DELTA2-isomerase (1994), Arch. Microbiol., 161, 239-245.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.120 Dithionite inactivation, oxidation with potassium hexacyanoferrate(III) results in up to 84% reactivation Clostridium kluyveri

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.120 soluble high enzymic activity in anaerobically grown cell extract grown on succinate plus ethanol Clostridium kluyveri
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.120 Iron 13.6 mol iron per mol of enzyme Clostridium kluyveri
5.3.3.3 Fe-S 0.8 mol Fe and 10.8 mol S per mol of enzyme, iron-sulfur protein, bifunctional enzyme possessing 4-hydroxybutyryl-CoA dehydratase and vinylacetyl DELTA-isomerase activity Clostridium kluyveri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.120 59000
-
 4 * 59000, SDS-PAGE Clostridium kluyveri
4.2.1.120 237000
-
 gel filtration Clostridium kluyveri
5.3.3.3 59000
-
 4 * 59000, bifunctional enzyme possessing 4-hydroxybutyryl-CoA dehydratase and vinylacetyl DELTA-isomerase activity, SDS-PAGE Clostridium kluyveri
5.3.3.3 237000
-
 bifunctional enzyme possessing 4-hydroxybutyryl-CoA dehydratase and vinylacetyl DELTA-isomerase activity, gel filtration Clostridium kluyveri

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.120 Clostridium kluyveri
-
-
-
5.3.3.3 Clostridium kluyveri
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.2.1.120 exposure to air leads to irreversible inactivation Clostridium kluyveri
5.3.3.3 oxygen irreversibly inactivates Clostridium kluyveri

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.120 purification under strictly anaerobic conditions Clostridium kluyveri
5.3.3.3 bifunctional enzyme possessing 4-hydroxybutyryl-CoA dehydratase and vinylacetyl DELTA-isomerase activity Clostridium kluyveri

Renatured (Commentary)

EC Number Renatured (Comment) Organism
4.2.1.120 enzyme is inactivated by dithionite, oxidation with potassium hexacyanoferrate(III) results in up to 84% reactivation Clostridium kluyveri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.120 7.38
-
-
 Clostridium kluyveri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.120 4-hydroxybutanoyl-CoA
-
 Clostridium kluyveri but-3-enoyl-CoA + H2O
-
 ?
4.2.1.120 additional information enzyme has an intrinsic vinylacetyl-CoA isomerase activity Clostridium kluyveri ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.120 tetramer 4 * 59000, SDS-PAGE Clostridium kluyveri
5.3.3.3 tetramer 4 * 59000, bifunctional enzyme possessing 4-hydroxybutyryl-CoA dehydratase and vinylacetyl DELTA-isomerase activity, SDS-PAGE Clostridium kluyveri

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.120 FAD 2 mol FAD per mol of enzyme Clostridium kluyveri
5.3.3.3 FAD 2 mol per mol of enzyme, bifunctional enzyme possessing 4-hydroxybutyryl-CoA dehydratase and vinylacetyl DELTA-isomerase activity Clostridium kluyveri