BRENDA - Enzyme Database

The enzymatic isomerization of beta-methylvinylacetyl coenzyme A and the specificity of a bacterial coenzyme A carboxylase

Rilling, H.C.; Coon, M.J.; J. Biol. Chem. 235, 3087-3092 (1960)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.3.3
beta-methylcrotonyl-CoA
-
Bos taurus
5.3.3.3
additional information
not inhibited by iodoacetate
Bos taurus
5.3.3.3
N-ethylmaleimide
-
Bos taurus
5.3.3.3
p-chloromercuribenzoate
-
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.3.3
0.05
-
vinylacetyl-CoA
beta-methylvinylacetyl-CoA
Bos taurus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
5.3.3.3
additional information
not influenced by Na+ or K+
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.3.3
Bos taurus
-
-
-
5.3.3.3
Gallus gallus
-
-
-
5.3.3.3
Pseudomonas sp.
-
-
-
5.3.3.3
Sus scrofa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.3.3
-
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.3.3
heart
-
Sus scrofa
-
5.3.3.3
kidney
-
Sus scrofa
-
5.3.3.3
liver
-
Bos taurus
-
5.3.3.3
liver
-
Gallus gallus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.3.3.3
32
-
substrate beta-vinylacetyl-CoA
Bos taurus
Storage Stability
EC Number
Storage Stability
Organism
5.3.3.3
frozen or at 5°C, stable for months
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.3.3
3-Hexenoyl-CoA
-
2960
Bos taurus
?
-
-
-
-
5.3.3.3
Beta-Methylvinylacetyl-CoA
ir
2960
Bos taurus
Beta-Methylvinylacetyl-CoA
-
2960
Bos taurus
-
5.3.3.3
additional information
not 4-pentenoyl-CoA, beta-methylvinylacetate, beta-vinylacetyl ethyl ester, 5-androstan-3,17-dione, 5-cholestenone, 5-pregnene-3,20-dione
2960
Bos taurus
?
-
-
-
-
5.3.3.3
Vinylacetyl pantetheine
-
2960
Bos taurus
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3.3.3
8
9
-
Bos taurus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.3.3.3
additional information
no cofactor
Bos taurus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.3.3.3
additional information
no cofactor
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.3.3
beta-methylcrotonyl-CoA
-
Bos taurus
5.3.3.3
additional information
not inhibited by iodoacetate
Bos taurus
5.3.3.3
N-ethylmaleimide
-
Bos taurus
5.3.3.3
p-chloromercuribenzoate
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.3.3
0.05
-
vinylacetyl-CoA
beta-methylvinylacetyl-CoA
Bos taurus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
5.3.3.3
additional information
not influenced by Na+ or K+
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.3.3
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.3.3
heart
-
Sus scrofa
-
5.3.3.3
kidney
-
Sus scrofa
-
5.3.3.3
liver
-
Bos taurus
-
5.3.3.3
liver
-
Gallus gallus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.3.3.3
32
-
substrate beta-vinylacetyl-CoA
Bos taurus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
5.3.3.3
frozen or at 5°C, stable for months
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.3.3
3-Hexenoyl-CoA
-
2960
Bos taurus
?
-
-
-
-
5.3.3.3
Beta-Methylvinylacetyl-CoA
ir
2960
Bos taurus
Beta-Methylvinylacetyl-CoA
-
2960
Bos taurus
-
5.3.3.3
additional information
not 4-pentenoyl-CoA, beta-methylvinylacetate, beta-vinylacetyl ethyl ester, 5-androstan-3,17-dione, 5-cholestenone, 5-pregnene-3,20-dione
2960
Bos taurus
?
-
-
-
-
5.3.3.3
Vinylacetyl pantetheine
-
2960
Bos taurus
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3.3.3
8
9
-
Bos taurus