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Literature summary extracted from

  • Goldberg, A.L.; Swamy, K.H.S.; Chung, C.H.; Larimore, F.S.
    Proteases in Escherichia coli (1981), Methods Enzymol., 80, 680-702.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.67 DFP
-
 Escherichia coli
3.4.21.67 additional information not: metal chelating agents; sulfhydryl inhibitors; tosyllysine chloromethyl ketone Escherichia coli
3.4.21.67 Pentanamide
-
 Escherichia coli
3.4.21.67 PMSF
-
 Escherichia coli
3.4.21.67 tosylphenylalanine chloromethyl ketone
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.67 cytoplasm
-
 Escherichia coli 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.67 70000
-
 2 * 70000, SDS-PAGE Escherichia coli
3.4.21.67 140000
-
 E. coli, gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.67 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.67
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.67 additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.67 Methyl-alpha-casein + H2O
-
 Escherichia coli Hydrolyzed methyl-alpha-casein
-
 ?
3.4.21.67 Methylhemoglobin + H2O
-
 Escherichia coli Hydrolyzed methylhemoglobin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.67 dimer 2 * 70000, SDS-PAGE Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.67 6.5
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.67 6 8
-
 Escherichia coli