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Literature summary extracted from
- Comparative study of various serine alkaline proteinases from microorganisms. Esterase activity against N-acylated peptide ester substrates (1974), Arch. Biochem. Biophys., 165, 72-79.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.63 | Benzyloxycarbonyl-Ala-Gly-PheCH2Cl | - |
Aspergillus melleus |  |
| 3.4.21.63 | Benzyloxycarbonyl-Ala-Gly-PheCH2Cl | - |
Aspergillus sojae | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.63 | additional information | - |
additional information | - |
Aspergillus sojae | |
| 3.4.21.63 | additional information | - |
additional information | - |
Aspergillus melleus | |
| 3.4.21.63 | 4.7 | - |
acetyl-(Ala)4 methyl ester | Aspergillus melleus | Aspergillus melleus | |
| 3.4.21.63 | 6.7 | - |
acetyl-(Ala)3 methyl ester | Aspergillus melleus | Aspergillus melleus | |
| 3.4.21.63 | 6.7 | - |
acetyl-(Ala)3 methyl ester | acetyl-(Ala)2-Phe methyl ester, Aspergillus sojae | Aspergillus sojae | |
| 3.4.21.63 | 7 | - |
acetyl-(Ala)4 methyl ester | Aspergillus sojae | Aspergillus sojae | |
| 3.4.21.63 | 13.5 | - |
Acetyl-Ala-Phe methyl ester | Aspergillus sojae | Aspergillus sojae | |
| 3.4.21.63 | 23 | - |
Acetyl-Phe methyl ester | Aspergillus sojae | Aspergillus sojae | |
| 3.4.21.63 | 32.1 | - |
acetyl-(Ala)3 methyl ester | Aspergillus sojae | Aspergillus sojae | |
| 3.4.21.63 | 44.5 | - |
acetyl-(Ala)2 methyl ester | Aspergillus sojae | Aspergillus sojae | |
| 3.4.21.63 | 68.5 | - |
acetyl-(Ala)2 methyl ester | Aspergillus melleus | Aspergillus melleus | |
| 3.4.21.63 | 96.8 | - |
Acetyl-Ala methyl ester | Aspergillus sojae | Aspergillus sojae | |
| 3.4.21.63 | 194 | - |
acetyl-(Ala)4 methyl ester | Aspergillus melleus | Aspergillus melleus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.63 | Aspergillus melleus | - |
- |
- |
| 3.4.21.63 | Aspergillus sojae | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.63 | Acetyl-Ala methyl ester + H2O | - |
Aspergillus sojae | Acetyl-Ala + methanol | - |
? | |
| 3.4.21.63 | Acetyl-Ala methyl ester + H2O | - |
Aspergillus melleus | Acetyl-Ala + methanol | - |
? | |
| 3.4.21.63 | Acetyl-Ala-Ala methyl ester + H2O | - |
Aspergillus sojae | Acetyl-Ala-Ala + methanol | - |
? | |
| 3.4.21.63 | Acetyl-Ala-Ala methyl ester + H2O | - |
Aspergillus melleus | Acetyl-Ala-Ala + methanol | - |
? | |
| 3.4.21.63 | Acetyl-Ala-Ala-Ala methyl ester + H2O | - |
Aspergillus sojae | Acetyl-Ala-Ala-Ala + methanol | - |
? | |
| 3.4.21.63 | Acetyl-Ala-Ala-Ala methyl ester + H2O | - |
Aspergillus melleus | Acetyl-Ala-Ala-Ala + methanol | - |
? | |
| 3.4.21.63 | Acetyl-Ala-Ala-Ala-Ala methyl ester + H2O | - |
Aspergillus sojae | Acetyl-Ala-Ala-Ala-Ala + methanol | - |
? | |
| 3.4.21.63 | Acetyl-Ala-Ala-Ala-Ala methyl ester + H2O | - |
Aspergillus melleus | Acetyl-Ala-Ala-Ala-Ala + methanol | - |
? | |
| 3.4.21.63 | Acetyl-Ala-Ala-Phe methyl ester + H2O | - |
Aspergillus sojae | Acetyl-Ala-Ala-Phe + methanol | - |
? | |
| 3.4.21.63 | Acetyl-Ala-Ala-Phe methyl ester + H2O | - |
Aspergillus melleus | Acetyl-Ala-Ala-Phe + methanol | - |
? | |
| 3.4.21.63 | Acetyl-Ala-Phe methyl ester + H2O | - |
Aspergillus sojae | Acetyl-Ala-Phe + methanol | - |
? | |
| 3.4.21.63 | Acetyl-Ala-Phe methyl ester + H2O | - |
Aspergillus melleus | Acetyl-Ala-Phe + methanol | - |
? | |
| 3.4.21.63 | Acetyl-Phe methyl ester + H2O | - |
Aspergillus sojae | Acetyl-Phe + methanol | - |
? | |
| 3.4.21.63 | Acetyl-Phe methyl ester + H2O | - |
Aspergillus melleus | Acetyl-Phe + methanol | - |
? | |
| 3.4.21.63 | Benzyloxycarbonyl-Ala-Ala-Lys methyl ester + H2O | - |
Aspergillus sojae | Benzyloxycarbonyl-Ala-Ala-Lys + methanol | - |
? | |
| 3.4.21.63 | Benzyloxycarbonyl-Ala-Ala-Lys methyl ester + H2O | - |
Aspergillus melleus | Benzyloxycarbonyl-Ala-Ala-Lys + methanol | - |
? | |
| 3.4.21.63 | Benzyloxycarbonyl-Ala-Lys methyl ester + H2O | - |
Aspergillus sojae | Benzyloxycarbonyl-Ala-Lys + methanol | - |
? | |
| 3.4.21.63 | Benzyloxycarbonyl-Ala-Lys methyl ester + H2O | - |
Aspergillus melleus | Benzyloxycarbonyl-Ala-Lys + methanol | - |
? | |
| 3.4.21.63 | Benzyloxycarbonyl-Gly-Lys methyl ester + H2O | - |
Aspergillus sojae | Benzyloxycarbonyl-Gly-Lys + methanol | - |
? | |
| 3.4.21.63 | Benzyloxycarbonyl-Gly-Lys methyl ester + H2O | - |
Aspergillus melleus | Benzyloxycarbonyl-Gly-Lys + methanol | - |
? | |
| 3.4.21.63 | Benzyloxycarbonyl-Leu-Lys methyl ester + H2O | - |
Aspergillus sojae | Benzyloxycarbonyl-Leu-Lys + methanol | - |
? | |
| 3.4.21.63 | Benzyloxycarbonyl-Leu-Lys methyl ester + H2O | - |
Aspergillus melleus | Benzyloxycarbonyl-Leu-Lys + methanol | - |
? | |
| 3.4.21.63 | Benzyloxycarbonyl-Lys methyl ester + H2O | - |
Aspergillus sojae | Benzyloxycarbonyl-Lys + methanol | - |
? | |
| 3.4.21.63 | Benzyloxycarbonyl-Lys methyl ester + H2O | - |
Aspergillus melleus | Benzyloxycarbonyl-Lys + methanol | - |
? | |
| 3.4.21.63 | Benzyloxycarbonyl-Phe-Lys methyl ester + H2O | - |
Aspergillus sojae | Benzyloxycarbonyl-Phe-Lys + methanol | - |
? | |
| 3.4.21.63 | Benzyloxycarbonyl-Phe-Lys methyl ester + H2O | - |
Aspergillus melleus | Benzyloxycarbonyl-Phe-Lys + methanol | - |
? | |
| 3.4.21.63 | additional information | the esterase activity is markedly promoted by elongating the peptide chain from P1 to P2 or P3 with alanine, irrespectively of the kind of the amino acid residue at the P1-position | Aspergillus sojae | ? | - |
? | |
| 3.4.21.63 | additional information | the esterase activity is markedly promoted by elongating the peptide chain from P1 to P2 or P3 with alanine, irrespectively of the kind of the amino acid residue at the P1-position | Aspergillus melleus | ? | - |
? | |
| 3.4.21.63 | additional information | leucine or phenylalanine is most efficient at the P2-position | Aspergillus sojae | ? | - |
? | |
| 3.4.21.63 | additional information | leucine or phenylalanine is most efficient at the P2-position | Aspergillus melleus | ? | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.63 | 28.3 | - |
Acetyl-Ala methyl ester | Aspergillus melleus | Aspergillus melleus | |
| 3.4.21.63 | 28.9 | - |
Acetyl-Ala methyl ester | Aspergillus sojae | Aspergillus sojae | |
| 3.4.21.63 | 1150 | - |
acetyl-(Ala)2 methyl ester | Aspergillus sojae | Aspergillus sojae | |
| 3.4.21.63 | 1540 | - |
acetyl-(Ala)3 methyl ester | Aspergillus melleus | Aspergillus melleus | |
| 3.4.21.63 | 1970 | - |
acetyl-(Ala)4 methyl ester | Aspergillus melleus | Aspergillus melleus | |
| 3.4.21.63 | 2000 | - |
acetyl-(Ala)2 methyl ester | Aspergillus melleus | Aspergillus melleus | |
| 3.4.21.63 | 2090 | - |
acetyl-(Ala)4 methyl ester | Aspergillus sojae | Aspergillus sojae | |
| 3.4.21.63 | 3720 | - |
acetyl-(Ala)3 methyl ester | Aspergillus sojae | Aspergillus sojae | |
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