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Literature summary extracted from

  • Thomas, P.G.; Russell, A.J.; Fersht, A.R.
    Tailoring the pH dependence of enzyme catalysis using protein engineering (1985), Nature, 318, 375-376.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.62
-
 Bacillus amyloliquefaciens
3.4.21.62
-
 Bacillus sp. (in: firmicutes)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.62 0.13
-
 succinyl-Ala-Ala-Pro-Phe 4-nitroanilide
-
 Bacillus sp. (in: firmicutes)
3.4.21.62 0.13
-
 succinyl-Ala-Ala-Pro-Phe 4-nitroanilide mutant subtilisin BPN' Asp to Ser99 Bacillus amyloliquefaciens
3.4.21.62 0.15
-
 MeO-succinyl-Ala-Ala-Phe 4-nitroanilide
-
 Bacillus sp. (in: firmicutes)
3.4.21.62 0.15
-
 MeO-succinyl-Ala-Ala-Phe 4-nitroanilide succinyl-Ala-Ala-Pro-Phe 4-nitroanilide, subtilisin BPN' Bacillus amyloliquefaciens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.62 Bacillus amyloliquefaciens
-
 wild-type and mutant (Asp to Ser99), the wild-type subtilisin BPN' is expressed and secreted by Bacillus subtilis DB104
-
3.4.21.62 Bacillus sp. (in: firmicutes)
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.62 MeO-succinyl-Ala-Ala-Phe 4-nitroanilide + H2O
-
 Bacillus sp. (in: firmicutes) ?
-
 ?
3.4.21.62 MeO-succinyl-Ala-Ala-Phe 4-nitroanilide + H2O
-
 Bacillus amyloliquefaciens ?
-
 ?
3.4.21.62 succinyl-Ala-Ala-Pro-Phe 4-nitroanilide + H2O
-
 Bacillus sp. (in: firmicutes) succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
3.4.21.62 succinyl-Ala-Ala-Pro-Phe 4-nitroanilide + H2O
-
 Bacillus amyloliquefaciens succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.62 45
-
 succinyl-Ala-Ala-Pro-Phe 4-nitroanilide mutant subtilisin BPN' Asp99Ser Bacillus amyloliquefaciens
3.4.21.62 57
-
 MeO-succinyl-Ala-Ala-Phe 4-nitroanilide subtilisin Carlsberg Bacillus sp. (in: firmicutes)
3.4.21.62 57
-
 MeO-succinyl-Ala-Ala-Phe 4-nitroanilide subtilisin Carlsberg Bacillus amyloliquefaciens
3.4.21.62 57
-
 succinyl-Ala-Ala-Pro-Phe 4-nitroanilide subtilisin BPN' Bacillus sp. (in: firmicutes)
3.4.21.62 57
-
 succinyl-Ala-Ala-Pro-Phe 4-nitroanilide subtilisin BPN' Bacillus amyloliquefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.62 additional information
-
-
 Bacillus sp. (in: firmicutes)
3.4.21.62 additional information
-
 mutant subtilisin Asp to Ser99, change of just one surface charge which is 14-15 A from the active site of subtilisin has a significant effect on the pH-dependence Bacillus amyloliquefaciens