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Literature summary extracted from

  • Peters, K.; Pauli, D.; Hache, H.; Boteva, R.N.; Genov, N.C.; Fittkau, S.
    Subtilisin DY – kinetic characterization and comparison with related proteinases (1989), Curr. Microbiol., 18, 171-177.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.62 Benzyloxycarbonyl-(Ala)n-PheCH2Cl benzyloxycarbonyl-(Ala)2-PheCH2Cl is the best inhibitor Bacillus sp. (in: Bacteria)
3.4.21.62 Benzyloxycarbonyl-Ala-XaaCH2Cl Xaa : Gly, Val, Ala, Leu, Phe Bacillus sp. (in: Bacteria)
3.4.21.62 Dipeptidyl chloromethyl ketones
-
 Bacillus sp. (in: Bacteria)
3.4.21.62 Peptidyl chloromethyl ketones
-
 Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.62 0.15
-
 MeO-succinyl-Ala-Ala-Phe 4-nitroanilide subtilisin DY Bacillus sp. (in: Bacteria)
3.4.21.62 0.22
-
 MeO-succinyl-Ala-Ala-Phe 4-nitroanilide subtilisin Carlsberg Bacillus sp. (in: Bacteria)
3.4.21.62 0.71
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide subtilisin DY Bacillus sp. (in: Bacteria)
3.4.21.62 0.79
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide subtilisin Carlsberg Bacillus sp. (in: Bacteria)
3.4.21.62 1.2
-
 Succinyl-Ala-Ala-Ala 4-nitroanilide subtilisin Carlsberg Bacillus sp. (in: Bacteria)
3.4.21.62 1.5
-
 Succinyl-Ala-Ala-Ala 4-nitroanilide subtilisin DY Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.62 Bacillus sp. (in: Bacteria)
-
 subtilisin from Boehringer
-
3.4.21.62 Bacillus sp. (in: Bacteria)
-
 subtilisin BPN' from Serva
-
3.4.21.62 Bacillus sp. (in: Bacteria)
-
 subtilisin DY
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.62 commercial preparation from Serva Bacillus sp. (in: Bacteria)
-
3.4.21.62 commercial preparation from Boehringer Bacillus sp. (in: Bacteria)
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.62 MeO-succinyl-Ala-Ala-Phe 4-nitroanilide + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 Succinyl-Ala-Ala 4-nitroanilide + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 Succinyl-Ala-Ala-Ala 4-nitroanilide + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 succinyl-Ala-Ala-Phe-4-nitroanilide + H2O
-
 Bacillus sp. (in: Bacteria) succinyl-Ala-Ala-Phe + 4-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.62 Subtilisin DY
-
 Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.62 1.6
-
 Succinyl-Ala-Ala-Ala 4-nitroanilide subtilisin Carlsberg Bacillus sp. (in: Bacteria)
3.4.21.62 1.8
-
 Succinyl-Ala-Ala-Ala 4-nitroanilide subtilisin DY Bacillus sp. (in: Bacteria)
3.4.21.62 47
-
 MeO-succinyl-Ala-Ala-Phe 4-nitroanilide subtilisin DY Bacillus sp. (in: Bacteria)
3.4.21.62 52
-
 succinyl-Ala-Ala-Phe-4-nitroanilide subtilisin DY Bacillus sp. (in: Bacteria)
3.4.21.62 97
-
 MeO-succinyl-Ala-Ala-Phe 4-nitroanilide subtilisin Bacillus sp. (in: Bacteria)