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Literature summary extracted from

  • Dogbo, O.; Camara, B.
    Purification of isopentenyl pyrophosphate isomerase and geranylgeranyl pyrophosphate synthase from Capsicum chromoplasts by affinity chromatography (1987), Biochim. Biophys. Acta, 920, 140-148.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.29 additional information inhibition by irradiation with the photolabile analog of geranyl diphosphate Capsicum annuum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.29 0.00095
-
 dimethylallyl diphosphate
-
 Capsicum annuum
2.5.1.29 0.001
-
 geranyl diphosphate
-
 Capsicum annuum
2.5.1.29 0.0012
-
 farnesyl diphosphate
-
 Capsicum annuum
2.5.1.29 0.003
-
 isopentenyl diphosphate
-
 Capsicum annuum
5.3.3.2 0.006
-
 isopentenyl diphosphate
-
 Capsicum annuum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.29 chromoplast stroma Capsicum annuum 9509
-
5.3.3.2 chromoplast stroma Capsicum annuum 9509
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.29 37000
-
 2 * 37000, SDS-PAGE Capsicum annuum
2.5.1.29 74000
-
 gel filtration Capsicum annuum
5.3.3.2 33500
-
 gel filtration Capsicum annuum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.29 (E,E)-farnesyl diphosphate + isopentenyl diphosphate Capsicum annuum
-
 geranylgeranyl diphosphate + diphosphate
-
 ?
2.5.1.29 dimethylallyl diphosphate + 3 isopentenyl diphosphate Capsicum annuum
-
 geranylgeranyl diphosphate + 3 diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate Capsicum annuum
-
 geranylgeranyl diphosphate + 2 diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.29 Capsicum annuum
-
-
-
5.3.3.2 Capsicum annuum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.29 poly(ethylene glycol) 6000, DEAE-Sephacel, aminophenethyl diphosphate affinity column Capsicum annuum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.3.3.2 fruit
-
 Capsicum annuum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.29 0.017
-
-
 Capsicum annuum
5.3.3.2 additional information
-
-
 Capsicum annuum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.29 (E,E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Capsicum annuum geranylgeranyl diphosphate + diphosphate
-
 ?
2.5.1.29 dimethylallyl diphosphate + 3 isopentenyl diphosphate
-
 Capsicum annuum geranylgeranyl diphosphate + 3 diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate
-
 Capsicum annuum geranylgeranyl diphosphate + 2 diphosphate
-
 ?
5.3.3.2 isopentenyl diphosphate
-
 Capsicum annuum dimethylallyl diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.29 dimer 2 * 37000, SDS-PAGE Capsicum annuum
5.3.3.2 monomer 1 * 33500, SDS-PAGE Capsicum annuum