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Literature summary extracted from
- The subtilisins (1970), Methods Enzymol., 19, 199-215.
No PubMed abstract available
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.21.62 | Relatively resistant to denaturation by guanidine solutions | Bacillus sp. (in: Bacteria) |
| 3.4.21.62 | Urea, 4 M, no effect | Bacillus sp. (in: Bacteria) |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.62 | 4-(4'-Aminophenylazo)phenylarsonic acid | - |
Bacillus sp. (in: Bacteria) |  |
| 3.4.21.62 | acetone | - |
Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | acetonitrile | - |
Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | Broad bean extract | subtilisin BPN' | Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | Chymotrypsin I inhibitor from potato | subtilisin Carlsberg, subtilisin BPN' | Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | Cyclohexanol | - |
Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | cyclohexanone | - |
Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | DFP | - |
Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | Hydrocinnamate | - |
Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | indole | - |
Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | Inhibitor from egg white | subtilisin Carlsberg, subtilisin BPN' | Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | additional information | not: tosyl-leucine chloromethyl ketone | Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | p-Arsanilate | - |
Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | p-Nitrophenylarsonate | - |
Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | p-Tolylarsonate | - |
Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | phenol | - |
Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | Phenylarsonate | - |
Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | phenylmethanesulfonyl fluoride | - |
Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | phenylmethanesulfonyl fluoride | - |
Bacillus subtilis | |
| 3.4.21.62 | Potato extract | subtilisin BPN' | Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | tosyl-Phe chloromethyl ketone | not | Bacillus sp. (in: Bacteria) | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.62 | 7 | - |
benzoyl-Arg ethyl ester | subtilisin Novo, subtilisin Carlsberg | Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | 10 | - |
benzoyl-Arg ethyl ester | subtilisn BPN' | Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | 17 | - |
N-acetyl-Phe ethyl ester | subtilisin BPN' | Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | 22 | - |
N-Acetyl-Tyr ethyl ester | subtilisin BPN' | Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | 24 | - |
N-Acetyl-Trp ethyl ester | subtilisin BPN' | Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | 30 | - |
toluenesulfonyl-Arg methyl ester | subtilisin Novo, N-acetyl-Phe methyl ester, subtilisin Carlsberg | Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | 40 | - |
toluenesulfonyl-Arg methyl ester | subtilisin Carlsberg | Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | 50 | - |
N-Acetyl-Trp methyl ester | subtilisin Carlsberg | Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | 60 | - |
N-Acetyl-Phe methyl ester | subtilisin Novo | Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | 66 | - |
N-Acetyl-Leu methyl ester | subtilisin BPN' | Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | 70 | - |
N-Acetyl-Tyr ethyl ester | subtilisin Novo, N-acetyl-Tyr methyl ester, subtilisin Carlsberg | Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | 90 | - |
N-Acetyl-Tyr methyl ester | subtilisin Novo, N-acetyl-Tyr ethyl ester, subtilisin Carlsberg, N-acetyl-Trp methyl ester, subtilisin Novo | Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | 91 | - |
N-Acetyl-Lys methyl ester | subtilisin BPN' | Bacillus sp. (in: Bacteria) | |
| 3.4.21.62 | 120 | - |
N-Acetyl-Ala methyl ester | subtilisin BPN' | Bacillus sp. (in: Bacteria) | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.21.62 | extracellular | - |
Bacillus subtilis | - |
- |
| 3.4.21.62 | extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
| 3.4.21.62 | extracellular | - |
Bacillus amyloliquefaciens | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.62 | additional information | enzyme contains no metal ion | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.62 | additional information | - |
amino acid sequence of: subtilisin BPN' | Bacillus subtilis |
| 3.4.21.62 | additional information | - |
amino acid sequence of: subtilisin BPN' | Bacillus sp. (in: Bacteria) |
| 3.4.21.62 | additional information | - |
amino acid sequence of: subtilisin BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | 27290 | - |
subtilisin Novo, determination from amino acid composition | Bacillus sp. (in: Bacteria) |
| 3.4.21.62 | 27530 | - |
Bacillus sp., subtilisin BPN', determination from amino acid composition | Bacillus sp. (in: Bacteria) |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.62 | Bacillus amyloliquefaciens | - |
- |
- |
| 3.4.21.62 | Bacillus sp. (in: Bacteria) | - |
subtilisin BPN' from Bacillus subtilis, the identity of this strain is later questioned and classified as a species of Bacillus amyloliquefaciens | - |
| 3.4.21.62 | Bacillus sp. (in: Bacteria) | - |
subtilisin Novo | - |
| 3.4.21.62 | Bacillus sp. (in: Bacteria) | - |
subtilopeptidase | - |
| 3.4.21.62 | Bacillus subtilis | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.21.62 | additional information | contains no carbohydrate | Bacillus subtilis |
| 3.4.21.62 | additional information | contains no carbohydrate | Bacillus sp. (in: Bacteria) |
| 3.4.21.62 | additional information | contains no carbohydrate | Bacillus amyloliquefaciens |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.62 | - |
Bacillus subtilis |
| 3.4.21.62 | overview: isolation procedure | Bacillus sp. (in: Bacteria) |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.21.62 | culture medium | - |
Bacillus subtilis | - |
| 3.4.21.62 | culture medium | - |
Bacillus sp. (in: Bacteria) | - |
| 3.4.21.62 | culture medium | - |
Bacillus amyloliquefaciens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.62 | additional information | - |
assay procedure for esterolytic ,subtilisin Carlsberg or subtilisin BPN', and proteolytic activity | Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.62 | Benzoyl-Arg ethyl ester + H2O | subtilisin Carlsberg, subtilisin Novo | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 3.4.21.62 | Hemoglobin + H2O | denatured | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 3.4.21.62 | additional information | reactions catalyzed: 1. peptide bond hydrolysis, 2. ester bond hydrolysis, 3. transesterification, 4. transpeptidation | Bacillus subtilis | ? | - |
? | |
| 3.4.21.62 | additional information | reactions catalyzed: 1. peptide bond hydrolysis, 2. ester bond hydrolysis, 3. transesterification, 4. transpeptidation | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 3.4.21.62 | additional information | reactions catalyzed: 1. peptide bond hydrolysis, 2. ester bond hydrolysis, 3. transesterification, 4. transpeptidation | Bacillus amyloliquefaciens | ? | - |
? | |
| 3.4.21.62 | N-Acetyl-Ala methyl ester + H2O | subtilisin BPN' | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 3.4.21.62 | N-Acetyl-Leu methyl ester + H2O | subtilisin BPN' | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 3.4.21.62 | N-Acetyl-Lys methyl ester + H2O | subtilisin BPN' | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 3.4.21.62 | N-Acetyl-Phe ethyl ester + H2O | subtilisin BPN' | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 3.4.21.62 | N-Acetyl-Phe methyl ester + H2O | subtilisin Carlsberg, subtilisin Novo | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 3.4.21.62 | N-Acetyl-Trp ethyl ester + H2O | subtilisin BPN' | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 3.4.21.62 | N-Acetyl-Trp methyl ester + H2O | subtilisin Carlsberg, subtilisin Novo | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 3.4.21.62 | N-Acetyl-Tyr ethyl ester + H2O | subtilisin Carlsberg, subtilisin Novo | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 3.4.21.62 | N-Acetyl-Tyr methyl ester + H2O | subtilisin Carlsberg, subtilisin Novo | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 3.4.21.62 | N-Acetyl-Val methyl ester + H2O | subtilisin Carlsberg, subtilisin Novo | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 3.4.21.62 | Toluenesulfonyl-Arg methyl ester + H2O | subtilisin Carlsberg, subtilisin Novo | Bacillus sp. (in: Bacteria) | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.62 | More | synonyms of subtilisin Carlsberg: subtilisin, subtilisin A, subtilopeptidase A, alcalase Novo, synonyms of subtilisin BPN': nagarse proteinase, Nagarse, BPN', subtilopeptidase C, synonyms of subtilisin Novo: bacterial proteinase Novo, subtilisin B, subtilopeptidase B | Bacillus subtilis |
| 3.4.21.62 | More | synonyms of subtilisin Carlsberg: subtilisin, subtilisin A, subtilopeptidase A, alcalase Novo, synonyms of subtilisin BPN': nagarse proteinase, Nagarse, BPN', subtilopeptidase C, synonyms of subtilisin Novo: bacterial proteinase Novo, subtilisin B, subtilopeptidase B | Bacillus sp. (in: Bacteria) |
| 3.4.21.62 | More | synonyms of subtilisin Carlsberg: subtilisin, subtilisin A, subtilopeptidase A, alcalase Novo, synonyms of subtilisin BPN': nagarse proteinase, Nagarse, BPN', subtilopeptidase C, synonyms of subtilisin Novo: bacterial proteinase Novo, subtilisin B, subtilopeptidase B | Bacillus amyloliquefaciens |
| 3.4.21.62 | Subtilisin amylosacchariticus | from Bacillus subtilis var. amylosacchariticus | Bacillus subtilis |
| 3.4.21.62 | Subtilisin amylosacchariticus | from Bacillus subtilis var. amylosacchariticus | Bacillus sp. (in: Bacteria) |
| 3.4.21.62 | Subtilisin amylosacchariticus | from Bacillus subtilis var. amylosacchariticus | Bacillus amyloliquefaciens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.62 | 55 | - |
rapid denaturation above | Bacillus sp. (in: Bacteria) |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.62 | 7 | 8 | denatured hemoglobin, subtilisin Novo, subtilisin BPN' | Bacillus sp. (in: Bacteria) |
| 3.4.21.62 | 10 | 11 | casein, subtilisin Carlsberg | Bacillus sp. (in: Bacteria) |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.62 | 1 | 2 | denaturation | Bacillus sp. (in: Bacteria) |
| 3.4.21.62 | 4 | - |
both active and denatured subtilisins are present simultaneously, rapid autolysis | Bacillus sp. (in: Bacteria) |
| 3.4.21.62 | 11 | - |
above, destabilization of enzyme structure | Bacillus sp. (in: Bacteria) |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.62 | additional information | no requirement for a cofactor | Bacillus sp. (in: Bacteria) |
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