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Literature summary extracted from
- The new enzymology of precursor processing endoproteases (1992), J. Biol. Chem., 267, 23435-23438.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.21.94 | - |
Homo sapiens |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.61 | Ca2+ | required | Saccharomyces cerevisiae |  |
| 3.4.21.61 | Ca2+ | 1 mM | Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.61 | alpha-mating factor + H2O | Saccharomyces cerevisiae | cleavage of dibasic sites | ? | - |
? | |
| 3.4.21.61 | Killer toxin precursors + H2O | Saccharomyces cerevisiae | cleavage of dibasic sites | ? | - |
? | |
| 3.4.21.93 | Pro-opiomelanocortin + H2O | Mus musculus | the enzyme together with prohormone convertase 2 represents the major secretory granule processing activity responsible for processing neuroendocrine precursors | ? | - |
? | |
| 3.4.21.93 | Pro-opiomelanocortin + H2O | Homo sapiens | the enzyme together with prohormone convertase 2 represents the major secretory granule processing activity responsible for processing neuroendocrine precursors | ? | - |
? | |
| 3.4.21.93 | Proinsulin + H2O | Mus musculus | the enzyme together with prohormone convertase 2 represents the major secretory granule processing activity responsible for processing neuroendocrine precursors | ? | - |
? | |
| 3.4.21.93 | Proinsulin + H2O | Homo sapiens | the enzyme together with prohormone convertase 2 represents the major secretory granule processing activity responsible for processing neuroendocrine precursors | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.61 | Saccharomyces cerevisiae | - |
- |
- |
| 3.4.21.75 | eukaryota | - |
at least 3 related enzymes are recognized in mammals: PC4 | - |
| 3.4.21.75 | eukaryota | - |
at least 3 related enzymes are recognized in mammals: PC3 (identical with PC1) | - |
| 3.4.21.75 | eukaryota | - |
at least 3 related enzymes are recognized in mammals: PC2 | - |
| 3.4.21.93 | Homo sapiens | - |
- |
- |
| 3.4.21.93 | Mus musculus | - |
- |
- |
| 3.4.21.94 | Homo sapiens | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.21.93 | AtT-20 cell | anterior | Mus musculus | - |
| 3.4.21.93 | pancreatic islet | - |
Mus musculus | - |
| 3.4.21.93 | pancreatic islet | - |
Homo sapiens | - |
| 3.4.21.94 | insulinoma cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.61 | alpha-mating factor + H2O | cleavage of dibasic sites | Saccharomyces cerevisiae | ? | - |
? | |
| 3.4.21.61 | Killer toxin precursors + H2O | cleavage of dibasic sites | Saccharomyces cerevisiae | ? | - |
? | |
| 3.4.21.61 | Protein + H2O | specificity: preference for Lys-Arg, while Arg-Arg, Pro-Arg, Ala-Arg, and Thr-Arg are equally rapidly cleaved but with higher Km | Saccharomyces cerevisiae | ? | - |
? | |
| 3.4.21.61 | Protein + H2O | autocatalytic activation at an internal Lys108-Arg109 | Saccharomyces cerevisiae | ? | - |
? | |
| 3.4.21.61 | Protein + H2O | cleaves a wide variety of precursors from higher eukaryotes including prohormones, such as proinsulin and proopiomelanocortin, as well as precursors of constitutively secreteted proteins, such as proalbumin | Saccharomyces cerevisiae | ? | - |
? | |
| 3.4.21.93 | Pro-opiomelanocortin + H2O | - |
Mus musculus | Adrenocorticotropic hormone + beta-lipotropin + beta endorphin | small amounts of beta-endorphin | ? | |
| 3.4.21.93 | Pro-opiomelanocortin + H2O | - |
Homo sapiens | Adrenocorticotropic hormone + beta-lipotropin + beta endorphin | small amounts of beta-endorphin | ? | |
| 3.4.21.93 | Pro-opiomelanocortin + H2O | the enzyme together with prohormone convertase 2 represents the major secretory granule processing activity responsible for processing neuroendocrine precursors | Mus musculus | ? | - |
? | |
| 3.4.21.93 | Pro-opiomelanocortin + H2O | the enzyme together with prohormone convertase 2 represents the major secretory granule processing activity responsible for processing neuroendocrine precursors | Homo sapiens | ? | - |
? | |
| 3.4.21.93 | Proinsulin + H2O | cleaves at both C peptide junctions to release rat insulin I but generates a larger proportion of intermediate cleaved at the B chain-C peptide junction, indicating a preference for this site | Mus musculus | Insulin + ? | - |
? | |
| 3.4.21.93 | Proinsulin + H2O | cleaves at both C peptide junctions to release rat insulin I but generates a larger proportion of intermediate cleaved at the B chain-C peptide junction, indicating a preference for this site | Homo sapiens | Insulin + ? | - |
? | |
| 3.4.21.93 | Proinsulin + H2O | the enzyme together with prohormone convertase 2 represents the major secretory granule processing activity responsible for processing neuroendocrine precursors | Mus musculus | ? | - |
? | |
| 3.4.21.93 | Proinsulin + H2O | the enzyme together with prohormone convertase 2 represents the major secretory granule processing activity responsible for processing neuroendocrine precursors | Homo sapiens | ? | - |
? | |
| 3.4.21.94 | Proinsulin + H2O | selective cleavage only at the C peptide-A chain junction | Homo sapiens | Insulin + ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.61 | More | similar enzymes occurs in mammals | Saccharomyces cerevisiae |
| 3.4.21.75 | More | one of a group of subtilisin homologues structurally and functionally similar to kexin | eukaryota |
| 3.4.21.75 | More | at least 3 related enzymes are recognized in mammals: PC2, PC3, identical with PC1and PC4, which have somewhat different specificities, the integral membrane protein furin may be of ancient origin, possibly ancestral to the secretory granule processing enzymes lacking transmembrane domains, such as PC2 and PC3 | eukaryota |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.61 | 37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.61 | 6.5 | 9.5 | - |
Saccharomyces cerevisiae |
| 3.4.21.93 | 5.5 | - |
- |
Mus musculus |
| 3.4.21.93 | 5.5 | - |
- |
Homo sapiens |
| 3.4.21.94 | 5.5 | - |
- |
Homo sapiens |
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