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Literature summary extracted from

  • Lemaire,G.; Gros, C.; Epely, S.; Kaminski, M.; Labouesse, B.
    Multiple forms of tryptophanyl-tRNA synthetase from beef pancreas (1975), Eur. J. Biochem., 51, 237-252.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.2 0.00038
-
 tRNATrp enzyme form TRS108, tRNA esterification Bos taurus
6.1.1.2 0.00045
-
 Trp enzyme form TRS82, tryptophan-dependent ATP-diphosphate exchange Bos taurus
6.1.1.2 0.0005
-
 Trp enzyme form TRS108, tryptophan-dependent ATP-diphosphate exchange Bos taurus
6.1.1.2 0.0008
-
 tRNATrp enzyme form TRS82, tRNA esterification Bos taurus
6.1.1.2 0.0025
-
 Trp enzyme form TRS108, tRNA esterification Bos taurus
6.1.1.2 0.005
-
 Trp enzyme form TRS82, tRNA esterification Bos taurus
6.1.1.2 0.15
-
 ATP enzyme form TRS108, tRNA esterification Bos taurus
6.1.1.2 0.5
-
 ATP enzyme form TRS82, tRNA esterification Bos taurus
6.1.1.2 2
-
 ATP enzyme form TRS108 and TRS92 Bos taurus
6.1.1.2 2
-
 ATP tryptophan-dependent ATP-diphosphate exchange Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.2 additional information
-
 multiple forms, possible the minor low MW forms (MW 82000 and 85000) are formed by limited proteolysis of the major form (MW 108000) Bos taurus
6.1.1.2 54000
-
 2 * 54000, enzyme form TRS108, SDS-PAGE Bos taurus
6.1.1.2 70000 83000 enzyme form TRS82, gel filtration, equilibrium sedimentation, gel electrophoresis Bos taurus
6.1.1.2 88000
-
 enzyme form TRS88, gel electrophoresis Bos taurus
6.1.1.2 108000
-
 gel filtration, enzyme form TRS108, equilibrium sedimentation Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.2 Bos taurus
-
 3 different forms: TRS108, TRS82 and TRS85
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.2
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.1.1.2 pancreas
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
6.1.1.2 -20°C, 5 mM phosphate buffer, pH 7, containing 300 mM KCl, 0.1 mM tryptophan, 0.1 mM EDTA and 1 mM mercaptoethanol Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.2 ATP + L-tryptophan + tRNATrp
-
 Bos taurus AMP + diphosphate + L-tryptophan-tRNATrp
-
 ?
6.1.1.2 additional information tryptophan-dependent ATP-diphosphate exchange: tryptophan + ATP + enzyme /Trp-AMP-enzyme + diphosphate Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.2 dimer 2 * 54000, enzyme form TRS108, SDS-PAGE Bos taurus