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Literature summary extracted from

  • Fiorucci, L.; Erba, F.; Ascoli, F.
    Bovine tryptase: purification and characterization (1992), Biol. Chem. Hoppe-Seyler, 373, 483-490.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.59 Aprotinin
-
 Bos taurus
3.4.21.59 Bovine pancreatic trypsin inhibitor pH-dependent Bos taurus
3.4.21.59 diisopropyl fluorophosphate
-
 Bos taurus
3.4.21.59 leupeptin
-
 Bos taurus
3.4.21.59 N-tosyl-L-Lys chloromethyl ketone
-
 Bos taurus
3.4.21.59 N-tosyl-L-Phe chloromethyl ketone weak Bos taurus
3.4.21.59 NaCl
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.59 0.0002
-
 Benzyloxycarbonyl-Ala-Arg-Arg 4-methylcoumarin 7-amide
-
 Bos taurus
3.4.21.59 0.0046
-
 Tert-Butyloxycarbonyl-Gly-Lys-Arg 4-methylcoumarin 7-amide
-
 Bos taurus
3.4.21.59 0.0231
-
 benzyloxycarbonyl-Arg 4-methylcoumarin 7-amide
-
 Bos taurus
3.4.21.59 0.029
-
 tert-butyloxycarbonyl-Phe-Ser-Arg 4-methylcoumarin 7-amide
-
 Bos taurus
3.4.21.59 0.031
-
 tert-butyloxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.59 39000
-
 x * 39000 + x * 40000, SDS-PAGE of DFP-labelled enzyme Bos taurus
3.4.21.59 40000
-
 x * 39000 + x * 40000, SDS-PAGE of DFP-labelled enzyme Bos taurus
3.4.21.59 360000
-
 gel filtration Bos taurus
3.4.21.59 360000
-
 bovine Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.59 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.59
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.59 mast cell
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.59 additional information
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.4.21.59 -20°C, high salt concentration, 50% glycerol, stable for some months Bos taurus
3.4.21.59 4°C, pH 6.1, high salt concentration, stable for about 2 or 3 weeks Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.59 Benzyloxycarbonyl-Ala-Arg-Arg 4-methylcoumarin 7-amide + H2O
-
 Bos taurus Benzyloxycarbonyl-Ala-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.59 Benzyloxycarbonyl-Arg 4-methylcoumarin 7-amide + H2O
-
 Bos taurus Benzyloxycarbonyl-Arg + 7-amino-4-methylcoumarine
-
 ?
3.4.21.59 N-tert-Butyloxycarbonyl-Phe-Ser-Arg 4-methylcoumarin 7-amide + H2O
-
 Bos taurus N-tert-Butyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.59 N-tert-Butyloxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O
-
 Bos taurus N-tert-Butyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.59 tert-butyloxycarbonyl-Gly-Lys-Arg 4-methylcoumarin 7-amide + H2O
-
 Bos taurus tert-butyloxycarbonyl-Gly-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.59 ? x * 39000 + x * 40000, SDS-PAGE of DFP-labelled enzyme Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.59 25
-
 assay at Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.59 0.05
-
 benzyloxycarbonyl-Arg 4-methylcoumarin 7-amide
-
 Bos taurus
3.4.21.59 0.5
-
 Tert-Butyloxycarbonyl-Gly-Lys-Arg 4-methylcoumarin 7-amide
-
 Bos taurus
3.4.21.59 0.8
-
 Benzyloxycarbonyl-Ala-Arg-Arg 4-methylcoumarin 7-amide
-
 Bos taurus
3.4.21.59 1.4
-
 tert-butyloxycarbonyl-L-Val-L-Pro-L-Arg-4-methylcoumarin 7-amide
-
 Bos taurus
3.4.21.59 2.2
-
 tert-butyloxycarbonyl-Phe-Ser-Arg 4-methylcoumarin 7-amide
-
 Bos taurus