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Literature summary extracted from

  • Alter, S.C.; Metcalfe, D.D.; Bradford, T.R.; Schwartz, L.B.
    Regulation of human mast cell tryptase. Effects of enzyme concentration, ionic strength and the structure and negative charge density of polysaccharides (1987), Biochem. J., 248, 821-827.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
3.4.21.59 chondroitin monosulfate stabilizes, prolongs t1/2 at 37°C 20fold Homo sapiens
3.4.21.59 chondroitin sulfate E stabilizes, prolongs t1/2 at 37°C 69fold Homo sapiens
3.4.21.59 heparan sufate stabilizes, prolongs t1/2 at 37°C 20fold Homo sapiens
3.4.21.59 heparin glycosaminoglycan fragments of MW greater than 5700 stabilizes Homo sapiens
3.4.21.59 increasing the NaCl concentration from 0.01 M to 1.0 M increases stability of free tryptase, in the presence of stabilizing polysaccharides it decreases the stability of tryptase until dissociation of tryptase from each polysaccharide occurs, thereafter tryptase stability increases as does that of free tryptase Homo sapiens
3.4.21.59 poly-D-glutamic acid stabilizes, prolongs t1/2 at 37°C 55fold: Dextran sulfate and heparin provide complete stabilization of tryptase activity for 120 min at 37°C Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.59 additional information
-
 additional information effect of NaCl on Km Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.59 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.59 Tosyl-Arg methyl ester + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.59 Tosyl-Gly-Pro-Lys 4-nitroanilide + H2O
-
 Homo sapiens Tosyl-Gly-Pro-Lys + 4-nitroaniline
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.59 additional information
-
 additional information effect of NaCl on turnover number Homo sapiens