BRENDA - Enzyme Database

Chymotrypsin- and trypsin-type serine proteases in rat mast cells: properties and functions

Kido, H.; Fukusen, N.; Katunuma, N.; Arch. Biochem. Biophys. 239, 436-443 (1985)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.21.39
alpha1-Aantichymotrypsin
-
Rattus norvegicus
3.4.21.39
alpha1-antitrypsin
-
Rattus norvegicus
3.4.21.39
antipain
-
Rattus norvegicus
3.4.21.39
Aprotinin
-
Rattus norvegicus
3.4.21.39
bestatin
-
Rattus norvegicus
3.4.21.39
decanoyl lysophosphatidic acid
-
Rattus norvegicus
3.4.21.39
diisopropyl fluorophosphate
-
Rattus norvegicus
3.4.21.39
dilauroyl phosphatidic acid
-
Rattus norvegicus
3.4.21.39
dioleoyl phosphatidic acid
-
Rattus norvegicus
3.4.21.39
dipalmitoyl phosphatidic acid
-
Rattus norvegicus
3.4.21.39
distearoyl phosphatidic acid
-
Rattus norvegicus
3.4.21.39
elaidic acid
-
Rattus norvegicus
3.4.21.39
linoleic acid
-
Rattus norvegicus
3.4.21.39
linolenic acid
-
Rattus norvegicus
3.4.21.39
oleamide
-
Rattus norvegicus
3.4.21.39
oleic acid
-
Rattus norvegicus
3.4.21.39
oleoyl lysophosphatidic acid
-
Rattus norvegicus
3.4.21.39
palmitoyl lysophosphatidic acid
-
Rattus norvegicus
3.4.21.39
phosphatidic acid
from bovine brain
Rattus norvegicus
3.4.21.39
phosphatidylethanolamine
-
Rattus norvegicus
3.4.21.39
phosphatidylinositol
-
Rattus norvegicus
3.4.21.39
phosphatidylserine
-
Rattus norvegicus
3.4.21.39
Soybean trypsin inhibitor
-
Rattus norvegicus
3.4.21.39
stearic acid
-
Rattus norvegicus
3.4.21.59
alpha1-antitrypsin
-
Rattus norvegicus
3.4.21.59
antipain
-
Rattus norvegicus
3.4.21.59
Aprotinin
-
Rattus norvegicus
3.4.21.59
diisopropyl fluorophosphate
-
Rattus norvegicus
3.4.21.59
leupeptin
-
Rattus norvegicus
3.4.21.59
Soybean trypsin inhibitor
-
Rattus norvegicus
3.4.21.59
Trypstatin
inhibition above pH 7.5
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.4.21.59
mast cell granule
-
Rattus norvegicus
42629
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.4.21.59
CaCl2
tryptase free from trypstatin is activated by 10 mM CaCl2, but tryptase associated with trypstatin is not
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.21.59
additional information
-
MW of the complex of tryptase and trypstatin: 144000, rat, gel filtration, MW of trypstatin is 7600 on gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.4.21.39
additional information
Rattus norvegicus
the enzyme plays a crucial or significant role in the process of degranulation
?
-
-
-
3.4.21.59
additional information
Rattus norvegicus
enzyme plays a crucial role in the process of degranulation
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.4.21.39
Rattus norvegicus
-
-
-
3.4.21.59
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.4.21.39
-
Rattus norvegicus
3.4.21.59
with an associated protein (trypstatin) that inhibits the protease activity above pH 7.5 (enzyme from peritoneal mast cells)
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.21.39
mast cell
-
Rattus norvegicus
-
3.4.21.39
peritoneum
-
Rattus norvegicus
-
3.4.21.59
mast cell
peritoneal
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.4.21.39
benzoyl-Arg-4-methylcoumarin 7-amide + H2O
1.2% of the activity with succinyl-Leu-Leu-Val-Tyr-4-methylcoumarin 7-amide
29374
Rattus norvegicus
benzoyl-Arg + 4-methylcoumarin 7-amide
-
-
-
?
3.4.21.39
additional information
the enzyme plays a crucial or significant role in the process of degranulation
29374
Rattus norvegicus
?
-
-
-
-
3.4.21.39
Pro-Phe-Arg-4-methylcoumarin 7-amide + H2O
4.6% of the activity with succinyl-Leu-Leu-Val-Tyr-4-methylcoumarin 7-amide
29374
Rattus norvegicus
?
-
-
-
?
3.4.21.39
Succinyl-Ala-Ala-Pro-Phe-4-methylcoumarin 7-amide + H2O
26.6% of the activity with succinyl-Leu-Leu-Val-Tyr-4-methylcoumarin 7-amide
29374
Rattus norvegicus
?
-
-
-
?
3.4.21.39
succinyl-Leu-Leu-Val-Tyr-4-methylcoumarin 7-amide + H2O
-
29374
Rattus norvegicus
?
-
-
-
?
3.4.21.39
t-butyloxycarbonyl-Val-Leu-Lys-4-methylcoumarin 7-amide + H2O
2.6% of the activity with succinyl-Leu-Leu-Val-Tyr-4-methylcoumarin 7-amide
29374
Rattus norvegicus
?
-
-
-
?
3.4.21.59
additional information
enzyme plays a crucial role in the process of degranulation
29374
Rattus norvegicus
?
-
-
-
-
3.4.21.59
N-tert-Butyloxycarbonyl-Ile-Glu-Gly-Arg 4-methylcoumarin 7-amide + H2O
rapid hydrolysis by free enzyme, scarcely hydrolyzed by the enzyme associated with trypstatin
29374
Rattus norvegicus
N-tert-Butyloxycarbonyl-Ile-Glu-Gly-Arg + 7-amino-4-methylcoumarin
-
-
-
-
3.4.21.59
N-tert-Butyloxycarbonyl-Phe-Ser-Arg 4-methylcoumarin 7-amide + H2O
-
29374
Rattus norvegicus
N-tert-Butyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
-
-
-
3.4.21.59
N-tert-Butyloxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O
-
29374
Rattus norvegicus
N-tert-Butyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.21.59
7.5
-
tryptase associated with trypstatin
Rattus norvegicus
3.4.21.59
8.5
-
tryptase free of trypstatin
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.21.39
alpha1-Aantichymotrypsin
-
Rattus norvegicus
3.4.21.39
alpha1-antitrypsin
-
Rattus norvegicus
3.4.21.39
antipain
-
Rattus norvegicus
3.4.21.39
Aprotinin
-
Rattus norvegicus
3.4.21.39
bestatin
-
Rattus norvegicus
3.4.21.39
decanoyl lysophosphatidic acid
-
Rattus norvegicus
3.4.21.39
diisopropyl fluorophosphate
-
Rattus norvegicus
3.4.21.39
dilauroyl phosphatidic acid
-
Rattus norvegicus
3.4.21.39
dioleoyl phosphatidic acid
-
Rattus norvegicus
3.4.21.39
dipalmitoyl phosphatidic acid
-
Rattus norvegicus
3.4.21.39
distearoyl phosphatidic acid
-
Rattus norvegicus
3.4.21.39
elaidic acid
-
Rattus norvegicus
3.4.21.39
linoleic acid
-
Rattus norvegicus
3.4.21.39
linolenic acid
-
Rattus norvegicus
3.4.21.39
oleamide
-
Rattus norvegicus
3.4.21.39
oleic acid
-
Rattus norvegicus
3.4.21.39
oleoyl lysophosphatidic acid
-
Rattus norvegicus
3.4.21.39
palmitoyl lysophosphatidic acid
-
Rattus norvegicus
3.4.21.39
phosphatidic acid
from bovine brain
Rattus norvegicus
3.4.21.39
phosphatidylethanolamine
-
Rattus norvegicus
3.4.21.39
phosphatidylinositol
-
Rattus norvegicus
3.4.21.39
phosphatidylserine
-
Rattus norvegicus
3.4.21.39
Soybean trypsin inhibitor
-
Rattus norvegicus
3.4.21.39
stearic acid
-
Rattus norvegicus
3.4.21.59
alpha1-antitrypsin
-
Rattus norvegicus
3.4.21.59
antipain
-
Rattus norvegicus
3.4.21.59
Aprotinin
-
Rattus norvegicus
3.4.21.59
diisopropyl fluorophosphate
-
Rattus norvegicus
3.4.21.59
leupeptin
-
Rattus norvegicus
3.4.21.59
Soybean trypsin inhibitor
-
Rattus norvegicus
3.4.21.59
Trypstatin
inhibition above pH 7.5
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.4.21.59
mast cell granule
-
Rattus norvegicus
42629
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.4.21.59
CaCl2
tryptase free from trypstatin is activated by 10 mM CaCl2, but tryptase associated with trypstatin is not
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.21.59
additional information
-
MW of the complex of tryptase and trypstatin: 144000, rat, gel filtration, MW of trypstatin is 7600 on gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.4.21.39
additional information
Rattus norvegicus
the enzyme plays a crucial or significant role in the process of degranulation
?
-
-
-
3.4.21.59
additional information
Rattus norvegicus
enzyme plays a crucial role in the process of degranulation
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.21.39
-
Rattus norvegicus
3.4.21.59
with an associated protein (trypstatin) that inhibits the protease activity above pH 7.5 (enzyme from peritoneal mast cells)
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.21.39
mast cell
-
Rattus norvegicus
-
3.4.21.39
peritoneum
-
Rattus norvegicus
-
3.4.21.59
mast cell
peritoneal
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.4.21.39
benzoyl-Arg-4-methylcoumarin 7-amide + H2O
1.2% of the activity with succinyl-Leu-Leu-Val-Tyr-4-methylcoumarin 7-amide
29374
Rattus norvegicus
benzoyl-Arg + 4-methylcoumarin 7-amide
-
-
-
?
3.4.21.39
additional information
the enzyme plays a crucial or significant role in the process of degranulation
29374
Rattus norvegicus
?
-
-
-
-
3.4.21.39
Pro-Phe-Arg-4-methylcoumarin 7-amide + H2O
4.6% of the activity with succinyl-Leu-Leu-Val-Tyr-4-methylcoumarin 7-amide
29374
Rattus norvegicus
?
-
-
-
?
3.4.21.39
Succinyl-Ala-Ala-Pro-Phe-4-methylcoumarin 7-amide + H2O
26.6% of the activity with succinyl-Leu-Leu-Val-Tyr-4-methylcoumarin 7-amide
29374
Rattus norvegicus
?
-
-
-
?
3.4.21.39
succinyl-Leu-Leu-Val-Tyr-4-methylcoumarin 7-amide + H2O
-
29374
Rattus norvegicus
?
-
-
-
?
3.4.21.39
t-butyloxycarbonyl-Val-Leu-Lys-4-methylcoumarin 7-amide + H2O
2.6% of the activity with succinyl-Leu-Leu-Val-Tyr-4-methylcoumarin 7-amide
29374
Rattus norvegicus
?
-
-
-
?
3.4.21.59
additional information
enzyme plays a crucial role in the process of degranulation
29374
Rattus norvegicus
?
-
-
-
-
3.4.21.59
N-tert-Butyloxycarbonyl-Ile-Glu-Gly-Arg 4-methylcoumarin 7-amide + H2O
rapid hydrolysis by free enzyme, scarcely hydrolyzed by the enzyme associated with trypstatin
29374
Rattus norvegicus
N-tert-Butyloxycarbonyl-Ile-Glu-Gly-Arg + 7-amino-4-methylcoumarin
-
-
-
-
3.4.21.59
N-tert-Butyloxycarbonyl-Phe-Ser-Arg 4-methylcoumarin 7-amide + H2O
-
29374
Rattus norvegicus
N-tert-Butyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
-
-
-
3.4.21.59
N-tert-Butyloxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O
-
29374
Rattus norvegicus
N-tert-Butyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.21.59
7.5
-
tryptase associated with trypstatin
Rattus norvegicus
3.4.21.59
8.5
-
tryptase free of trypstatin
Rattus norvegicus