Any feedback?
Literature summary extracted from
- Esterolytic properties of leucine-proteinase, the leucine-specific serine proteinase from spinach (Spinacia oleracea L.) leaves. A steady-state and pre-steady-state study (1986), Plant Physiol., 82, 591-593.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.57 | additional information | - |
additional information | Km values are pH-independent | Spinacia oleracea | |
| 3.4.21.57 | 0.018 | - |
Nalpha-carbobenzoxy-Leu 4-nitrophenyl ester | - |
Spinacia oleracea |  |
| 3.4.21.57 | 0.065 | - |
Nalpha-carbobenzoxy-Ile 4-nitrophenyl ester | - |
Spinacia oleracea | |
| 3.4.21.57 | 0.066 | - |
Nalpha-carbobenzoxy-Val 4-nitrophenyl ester | - |
Spinacia oleracea | |
| 3.4.21.57 | 0.12 | - |
Nalpha-carbobenzoxy-Ala 4-nitrophenyl ester | - |
Spinacia oleracea | |
| 3.4.21.57 | 0.26 | - |
Nalpha-carbobenzoxy-Lys 4-nitrophenyl ester | - |
Spinacia oleracea | |
| 3.4.21.57 | 0.28 | - |
Nalpha-carbobenzoxy-Arg 4-nitrophenyl ester | - |
Spinacia oleracea | |
| 3.4.21.57 | 0.52 | - |
Nalpha-carbobenzoxy-Gly 4-nitrophenyl ester | - |
Spinacia oleracea | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.57 | Spinacia oleracea | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.21.57 | leaf | - |
Spinacia oleracea | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.57 | Nalpha-carbobenzoxy-Ala 4-nitrophenyl ester + H2O | - |
Spinacia oleracea | Nalpha-carbobenzoxy-Ala + 4-nitrophenol | - |
? | |
| 3.4.21.57 | Nalpha-carbobenzoxy-Arg 4-nitrophenyl ester + H2O | - |
Spinacia oleracea | Nalpha-carbobenzoxy-Arg + 4-nitrophenol | - |
? | |
| 3.4.21.57 | Nalpha-carbobenzoxy-Gly 4-nitrophenyl ester + H2O | - |
Spinacia oleracea | Nalpha-carbobenzoxy-Gly + 4-nitrophenol | - |
? | |
| 3.4.21.57 | Nalpha-carbobenzoxy-Ile 4-nitrophenyl ester + H2O | - |
Spinacia oleracea | Nalpha-carbobenzoxy-Ile + 4-nitrophenol | - |
? | |
| 3.4.21.57 | Nalpha-carbobenzoxy-Leu 4-nitrophenyl ester + H2O | - |
Spinacia oleracea | Nalpha-carbobenzoxy-Leu + 4-nitrophenol | - |
? | |
| 3.4.21.57 | Nalpha-carbobenzoxy-Lys 4-nitrophenyl ester + H2O | - |
Spinacia oleracea | Nalpha-carbobenzoxy-Lys + 4-nitrophenol | - |
? | |
| 3.4.21.57 | Nalpha-carbobenzoxy-Val 4-nitrophenyl ester + H2O | - |
Spinacia oleracea | Nalpha-carbobenzoxy-Val + 4-nitrophenol | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.57 | additional information | - |
additional information | the acylation step is the rate-limiting in catalysis | Spinacia oleracea | |
| 3.4.21.57 | 21 | - |
Nalpha-carbobenzoxy-Gly 4-nitrophenyl ester | - |
Spinacia oleracea | |
| 3.4.21.57 | 43 | - |
Nalpha-carbobenzoxy-Arg 4-nitrophenyl ester | - |
Spinacia oleracea | |
| 3.4.21.57 | 45 | - |
Nalpha-carbobenzoxy-Lys 4-nitrophenyl ester | - |
Spinacia oleracea | |
| 3.4.21.57 | 94 | - |
Nalpha-carbobenzoxy-Ala 4-nitrophenyl ester | - |
Spinacia oleracea | |
| 3.4.21.57 | 140 | - |
Nalpha-carbobenzoxy-Ile 4-nitrophenyl ester | - |
Spinacia oleracea | |
| 3.4.21.57 | 140 | - |
Nalpha-carbobenzoxy-Val 4-nitrophenyl ester | - |
Spinacia oleracea | |
| 3.4.21.57 | 430 | - |
Nalpha-carbobenzoxy-Leu 4-nitrophenyl ester | - |
Spinacia oleracea | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.57 | 7.5 | - |
approximately | Spinacia oleracea |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
