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Literature summary extracted from

  • Menon, A.S.; Goldberg, A.L.
    Binding of nucleotides to the ATP-dependent protease La from Escherichia coli (1987), J. Biol. Chem., 262, 14921-14928.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.53 Adenyl-5'-yl imidodiphosphate i.e. AMP-PNP, activation Escherichia coli
3.4.21.53 Adenyl-5'-yl imidodiphosphate competes for one of the ATP-high-affinity binding-sites Escherichia coli
3.4.21.53 Adenyl-5'-yl imidodiphosphate peptide hydrolysis Escherichia coli
3.4.21.53 adenyl-5'-yl methylene monophosphonate i.e. AMP-CPP, activation, competes for the two ATP-high affinity sites Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.53 ADP ADP-binding in the presence of EDTA; kinetics; product inhibition Escherichia coli
3.4.21.53 vanadate decavanadate (not orthovanadate) Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.53 Mg2+ requirement Escherichia coli
3.4.21.53 Mg2+ 10 mM Escherichia coli
3.4.21.53 Mn2+ requirement, as Mn2+-ATP Escherichia coli
3.4.21.53 Mn2+ can replace Mg2+-ATP Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.53 Escherichia coli
-
 carrying lon-gene on the chromosome
-
3.4.21.53 Escherichia coli
-
 carrying lon-gene on plasmid pJMC40
-

Storage Stability

EC Number Storage Stability Organism
3.4.21.53 -70°C, in 40% glycerol, stable Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.53 casein + H2O
-
 Escherichia coli hydrolyzed casein
-
 ?
3.4.21.53 Glutaryl-Ala-Ala-Phe-methoxynaphthylamide + H2O
-
 Escherichia coli Glutaryl-Ala-Ala-Phe + methoxynaphthylamine
-
 ?
3.4.21.53 Succinyl-Phe-Ala-Phe-methoxynaphthylamide + H2O
-
 Escherichia coli Succinyl-Phe-Ala-Phe + methoxynaphthylamine
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
3.4.21.53 ATP requirement Escherichia coli
3.4.21.53 ATP only phosphate is released after ATP hydrolysis Escherichia coli
3.4.21.53 ATP ADP remains on the enzyme Escherichia coli
3.4.21.53 ATP ATP-binding is reversible Escherichia coli
3.4.21.53 ATP ATP- or ATP-analog-binding in the presence of EDTA Escherichia coli
3.4.21.53 ATP ATP-dependent protease Escherichia coli
3.4.21.53 ATP enzyme binds 4 mol ATP per tetramer at ATP concentrations above 0.01 mM Escherichia coli
3.4.21.53 ATP enzyme binds 2 mol ATP/tetramer in the absence of divalent cations or at 0.01 mM ATP in the presence of Mn2+ or Mg2+ Escherichia coli
3.4.21.53 ATP diphosphate, adenyl-5'-yl methylene diphosphate, GTP, UTP and CTP at 1 mM have no effect on the binding of ATP to the enzyme Escherichia coli