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Literature summary extracted from

  • Desautels, M.; Goldberg, A.L.
    Demonstration of an ATP-dependent, vanadate-sensitive endoprotease in the matrix of rat liver mitochondria (1982), J. Biol. Chem., 257, 11673-11679.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.53 GTP not Rattus norvegicus
3.4.21.53 additional information no activation by ubiquitin Rattus norvegicus
3.4.21.53 additional information ATP cannot be replaced by nucleoside diphosphates, nucleoside monophosphates, 5'-adenylyl-betagamma-methylene diphosphate, NADP+, NAD+ Rattus norvegicus

General Stability

EC Number General Stability Organism
3.4.21.53 2-mercaptoethanol stabilizes Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.53 azide weak Rattus norvegicus
3.4.21.53 chymostatin not (casein as substrate) Rattus norvegicus
3.4.21.53 diisopropyl fluorophosphate 10 mM, casein as substrate Rattus norvegicus
3.4.21.53 EDTA casein as substrate; strong Rattus norvegicus
3.4.21.53 iodoacetamide 10 mM, casein as substrate Rattus norvegicus
3.4.21.53 Mersalyl acid strong, 5 mM, casein as substrate Rattus norvegicus
3.4.21.53 additional information no inhibition by ubiquitin, bestatin, soybean trypsin inhibitor, aprotinin, leupeptin Rattus norvegicus
3.4.21.53 N,N'-dicyclohexylcarbodiimide weak Rattus norvegicus
3.4.21.53 N-ethylmaleimide not (casein as substrate) Rattus norvegicus
3.4.21.53 oligomycin weak Rattus norvegicus
3.4.21.53 PMSF 1 mM, casein as substrate Rattus norvegicus
3.4.21.53 quercetin 0.1 mM Rattus norvegicus
3.4.21.53 tosyl-Lys chloromethyl ketone not (casein as substrate) Rattus norvegicus
3.4.21.53 tosyl-Phe chloromethyl ketone not (casein as substrate) Rattus norvegicus
3.4.21.53 Triton X-100 0.05% Rattus norvegicus
3.4.21.53 vanadate 0.1 mM; casein as substrate Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.53 mitochondrion
-
 Rattus norvegicus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.53 diphosphate not Rattus norvegicus
3.4.21.53 Mg2+ requirement Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.53 550000
-
 rat, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.53 additional information Rattus norvegicus ATP-dependent serine protease ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.53 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.53 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.53 casein + H2O methylcasein Rattus norvegicus hydrolyzed casein
-
 ?
3.4.21.53 Methylglobin + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.21.53 additional information ATP-dependent serine protease Rattus norvegicus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.53 10
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.53 6.8 10 about half-maximal activity at pH 6.8 and maximal activity at pH 10, very little activity below 6 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
3.4.21.53 ATP at physiological concentrations Rattus norvegicus
3.4.21.53 ATP activation only in the presence of Mg2+ Rattus norvegicus
3.4.21.53 ATP ATP-dependent protease Rattus norvegicus
3.4.21.53 ATP ATP hydrolysis is essential for hydrolysis of proteins Rattus norvegicus
3.4.21.53 CTP activation Rattus norvegicus
3.4.21.53 CTP less efficient than ATP Rattus norvegicus
3.4.21.53 UTP activation Rattus norvegicus
3.4.21.53 UTP less efficient than ATP Rattus norvegicus