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Literature summary extracted from
- Studies of the ATP-dependent proteolytic enzyme, protease La, from Escherichia coli (1982), J. Biol. Chem., 257, 4187-4195.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.53 | GTP | activation | Escherichia coli |  |
| 3.4.21.53 | GTP | less efficient than ATP | Escherichia coli | |
| 3.4.21.53 | additional information | ATP cannot be replaced by ADP, AMP | Escherichia coli |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.21.53 | ADP and AMP bind to and stabilize the enzyme even in the absence of Mg2+ | Escherichia coli |
| 3.4.21.53 | ATP stabilizes | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.53 | 2',3'-dialdehyde-ATP | i.e. adenosine 2',3'-dialdehyde triphosphate, in the presence of ATP, globin as substrate | Escherichia coli | |
| 3.4.21.53 | adenosine 5'-(3-thiotriphosphate) | i.e. ATP-gamma-S, in the presence of ATP, globin as substrate | Escherichia coli | |
| 3.4.21.53 | ADP | prevents activation by ATP | Escherichia coli | |
| 3.4.21.53 | alpha,beta-methylene-ATP | in the presence of ATP, globin as substrate | Escherichia coli | |
| 3.4.21.53 | AMP | in the presence of ATP | Escherichia coli | |
| 3.4.21.53 | beta,gamma-methylene-ATP | in the presence of ATP, globin as substrate | Escherichia coli | |
| 3.4.21.53 | Dio-9 | ATPase inhibitor | Escherichia coli | |
| 3.4.21.53 | ethanol | - |
Escherichia coli | |
| 3.4.21.53 | KCl | weak, above 0.2 M | Escherichia coli | |
| 3.4.21.53 | additional information | termination factor rho or NaN3 | Escherichia coli | |
| 3.4.21.53 | N,N'-dicyclohexylcarbodiimide | not | Escherichia coli | |
| 3.4.21.53 | NaCl | weak, above 0.2 M | Escherichia coli | |
| 3.4.21.53 | oligomycin | weak | Escherichia coli | |
| 3.4.21.53 | phosphate | above 0.1 M | Escherichia coli | |
| 3.4.21.53 | quercetin | - |
Escherichia coli | |
| 3.4.21.53 | vanadate | - |
Escherichia coli | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.53 | 0.25 | - |
ATP | - |
Escherichia coli | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.21.53 | soluble | - |
Escherichia coli | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.53 | Ca2+ | can replace Mg2+-ATP | Escherichia coli | |
| 3.4.21.53 | Ca2+ | activation, as Ca2+-ATP | Escherichia coli | |
| 3.4.21.53 | Ca2+ | slightly less effective than Mg2+-ATP | Escherichia coli | |
| 3.4.21.53 | diphosphate | not | Escherichia coli | |
| 3.4.21.53 | Mg2+ | requirement | Escherichia coli | |
| 3.4.21.53 | Mg2+ | as Mg2+-ATP | Escherichia coli | |
| 3.4.21.53 | Mn2+ | requirement, as Mn2+-ATP | Escherichia coli | |
| 3.4.21.53 | Mn2+ | slightly more effective than Mg2+-ATP | Escherichia coli | |
| 3.4.21.53 | Mn2+ | can replace Mg2+-ATP | Escherichia coli | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.53 | Escherichia coli | - |
K-12 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.53 | partial | Escherichia coli |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.21.53 | 4°C, about 60% loss of activity within 4 days, 3 mM ATP stabilizes | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.53 | ATP + H2O | - |
Escherichia coli | phosphate + ADP | - |
? | |
| 3.4.21.53 | casein + H2O | alpha-casein | Escherichia coli | hydrolyzed casein | - |
? | |
| 3.4.21.53 | casein + H2O | methylcasein | Escherichia coli | hydrolyzed casein | - |
? | |
| 3.4.21.53 | Globin + H2O | - |
Escherichia coli | ? | - |
? | |
| 3.4.21.53 | Methylglobin + H2O | methyl-apohemoglobin | Escherichia coli | ? | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.53 | 42 | - |
1 h, 80% loss of activity, 3 mM ATP stabilizes, ADP or AMP less effectively, not Mg2+ | Escherichia coli |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.53 | ATP | requirement | Escherichia coli | |
| 3.4.21.53 | ATP | activation only in the presence of Mg2+ | Escherichia coli | |
| 3.4.21.53 | ATP | MgATP2- rather than ATP4- | Escherichia coli | |
| 3.4.21.53 | ATP | ATP-dependent protease | Escherichia coli | |
| 3.4.21.53 | ATP | MnATP2- or CaATP2- rather than ATP4- | Escherichia coli | |
| 3.4.21.53 | ATP | rapid depletion of ATP prevents proteolysis | Escherichia coli | |
| 3.4.21.53 | CTP | activation | Escherichia coli | |
| 3.4.21.53 | CTP | less efficient than ATP | Escherichia coli | |
| 3.4.21.53 | dATP | activation | Escherichia coli | |
| 3.4.21.53 | dATP | supports proteolysis with 75% the efficiency of ATP | Escherichia coli | |
| 3.4.21.53 | UTP | activation | Escherichia coli | |
| 3.4.21.53 | UTP | less efficient than ATP | Escherichia coli | |
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