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Literature summary extracted from
- Identification and purification of distinct isomerase and decarboxylase enzymes involved in the 4-hydroxyphenylacetate catabolic pathway of Escherichia coli (1981), Eur. J. Biochem., 117, 581-584.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.3.10 | additional information | enzyme is active in absence of Mg2+ | Escherichia coli |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.3.10 | 0.3 | - |
5-Carboxymethyl-2-hydroxymuconate | - |
Escherichia coli |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.68 | Mg2+ | - |
Escherichia coli | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.3.10 | 5-carboxymethyl-2-hydroxymuconate | Escherichia coli | enzyme of the 4-hydroxyphenylacetate meta-cleavage pathway | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.1.68 | Escherichia coli | - |
- |
- |
| 5.3.3.10 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.1.68 | by ammonium sulfate precipitation and gel filtration, additional purification is achieved using DEAE-Sephacel chromatography | Escherichia coli |
| 5.3.3.10 | - |
Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.10 | 108 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.68 | 5-carboxy-2-oxohept-3-enedioate + H+ | - |
Escherichia coli | 2-hydroxyhepta-2,4-dienedioate + CO2 | enzyme is part of the 4-hydroxyphenylacetate meta-cleavage pathway | ? | |
| 5.3.3.10 | 5-carboxymethyl-2-hydroxymuconate | - |
Escherichia coli | 5-carboxy-2-oxohept-3-enedioate | - |
? | |
| 5.3.3.10 | 5-carboxymethyl-2-hydroxymuconate | enzyme of the 4-hydroxyphenylacetate meta-cleavage pathway | Escherichia coli | ? | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.10 | 8 | 9 | - |
Escherichia coli |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 4.1.1.68 | metabolism | enzyme is part of the 4-hydroxyphenylacetate meta-cleavage pathway | Escherichia coli |
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