Any feedback?
Literature summary extracted from
- Isolation and purification of N-formylmethionine aminopeptidase from rat intestine (1992), Biochim. Biophys. Acta, 1119, 275-280.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.19.1 | Cd2+ | - |
Rattus norvegicus |  |
| 3.4.19.1 | Cu2+ | - |
Rattus norvegicus | |
| 3.4.19.1 | Hg2+ | - |
Rattus norvegicus | |
| 3.4.19.7 | 2-Benzyl succinate | - |
Rattus norvegicus | |
| 3.4.19.7 | Cd2+ | - |
Rattus norvegicus | |
| 3.4.19.7 | Cu2+ | markedly | Rattus norvegicus | |
| 3.4.19.7 | Fe2+ | not | Rattus norvegicus | |
| 3.4.19.7 | Hg2+ | - |
Rattus norvegicus | |
| 3.4.19.7 | additional information | 2-mercaptoethanol; DTT; N3-; not: EDTA | Rattus norvegicus | |
| 3.4.19.7 | phenylmethanesulfonyl fluoride | weak | Rattus norvegicus | |
| 3.4.19.7 | Zn2+ | moderate | Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.19.1 | 0.02 | - |
N-acetyl-Met-Leu | - |
Rattus norvegicus | |
| 3.4.19.1 | 0.04 | - |
formylmethionine-Leu | - |
Rattus norvegicus | |
| 3.4.19.1 | 0.06 | - |
formylmethionine-Val | - |
Rattus norvegicus | |
| 3.4.19.1 | 0.16 | - |
N-acetyl-Met-Leu-Phe | - |
Rattus norvegicus | |
| 3.4.19.1 | 0.18 | - |
formylmethionine-Leu-Phe | - |
Rattus norvegicus | |
| 3.4.19.1 | 0.22 | - |
formylmethionine-Leu-Tyr | - |
Rattus norvegicus | |
| 3.4.19.1 | 0.63 | - |
formylmethionine-Phe | - |
Rattus norvegicus | |
| 3.4.19.1 | 0.71 | - |
formylmethionine-Trp | - |
Rattus norvegicus | |
| 3.4.19.1 | 1.7 | - |
formylmethionine-beta-naphthylamide | - |
Rattus norvegicus | |
| 3.4.19.7 | 0.02 | - |
Acetyl-Met-Leu | - |
Rattus norvegicus | |
| 3.4.19.7 | 0.04 | - |
N-Formyl-Met-Leu | - |
Rattus norvegicus | |
| 3.4.19.7 | 0.06 | - |
N-Formyl-Met-Val | - |
Rattus norvegicus | |
| 3.4.19.7 | 0.16 | - |
Acetyl-Met-Leu-Phe | - |
Rattus norvegicus | |
| 3.4.19.7 | 0.18 | - |
N-Formyl-Met-Leu-Phe | - |
Rattus norvegicus | |
| 3.4.19.7 | 0.22 | - |
N-Formyl-Met-Leu-Tyr | - |
Rattus norvegicus | |
| 3.4.19.7 | 0.23 | - |
Formylnorleucine-Leu-Phe | - |
Rattus norvegicus | |
| 3.4.19.7 | 0.63 | - |
N-Formyl-Met-Phe | - |
Rattus norvegicus | |
| 3.4.19.7 | 0.71 | - |
N-Formyl-Met-Trp | - |
Rattus norvegicus | |
| 3.4.19.7 | 1.7 | - |
N-Formyl-Met 2-naphthylamide | - |
Rattus norvegicus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.19.1 | Cl- | activates | Rattus norvegicus | |
| 3.4.19.7 | Cl- | activates | Rattus norvegicus | |
| 3.4.19.7 | Cl- | maximal activity at 1 M | Rattus norvegicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.1 | 320000 | 340000 | gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.19.7 | N-Formylmethionyl oligopeptides + H2O | Rattus norvegicus | the presence of this enzyme in intestinal mucosa can prevent absorption of intact bioactive potent inflammatory N-formylmethionyl oligopeptides produced by commensal bacteria in the gut lumen | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.19.1 | Rattus norvegicus | - |
- |
- |
| 3.4.19.7 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.19.1 | - |
Rattus norvegicus |
| 3.4.19.7 | intestine | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.19.1 | intestine | mucosal epithelium | Rattus norvegicus | - |
| 3.4.19.7 | gut | lowest level in oesophagus and stomach and a shallow diminishing gradient from proximal small intestine to distal colon and rectum | Rattus norvegicus | - |
| 3.4.19.7 | intestine | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.1 | 12.86 | - |
- |
Rattus norvegicus |
| 3.4.19.7 | 12.86 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.19.1 | formylmethionine-beta-naphthylamide + H2O | - |
Rattus norvegicus | formylmethionine + beta-naphthylamine | - |
? | |
| 3.4.19.1 | formylmethionine-Leu + H2O | - |
Rattus norvegicus | formylmethionine + Leu | - |
? | |
| 3.4.19.1 | formylmethionine-Leu-Phe + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.19.1 | formylmethionine-Leu-Tyr + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.19.1 | formylmethionine-Phe + H2O | - |
Rattus norvegicus | formylmethionine + Phe | - |
? | |
| 3.4.19.1 | formylmethionine-Trp + H2O | - |
Rattus norvegicus | formylmethionine + Trp | - |
? | |
| 3.4.19.1 | formylmethionine-Val + H2O | - |
Rattus norvegicus | formylmethionine + Val | - |
? | |
| 3.4.19.1 | additional information | specific for N-terminal acylmethionine residues | Rattus norvegicus | ? | - |
? | |
| 3.4.19.1 | N-acetyl-Met-Leu + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.19.1 | N-acetyl-Met-Leu-Phe + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.19.7 | Acetyl-Met-Leu + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.19.7 | Acetyl-Met-Leu-Phe + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.19.7 | Formylnorleucine-Leu-Phe + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.19.7 | N-Formyl-Met 2-naphthylamide + H2O | - |
Rattus norvegicus | N-Formyl-Met + 2-naphthylamine | - |
? | |
| 3.4.19.7 | N-Formyl-Met-Leu + H2O | - |
Rattus norvegicus | N-Formyl-Met + Leu | - |
? | |
| 3.4.19.7 | N-Formyl-Met-Leu-Phe + H2O | - |
Rattus norvegicus | N-Formyl-Met + Leu-Phe | - |
? | |
| 3.4.19.7 | N-Formyl-Met-Leu-Tyr + H2O | - |
Rattus norvegicus | N-Formyl-Met + Leu-Tyr | - |
? | |
| 3.4.19.7 | N-Formyl-Met-Phe + H2O | - |
Rattus norvegicus | N-Formyl-Met + Phe | - |
? | |
| 3.4.19.7 | N-Formyl-Met-Trp + H2O | - |
Rattus norvegicus | N-Formyl-Met + Trp | - |
? | |
| 3.4.19.7 | N-Formyl-Met-Val + H2O | - |
Rattus norvegicus | N-Formyl-Met + Val | - |
? | |
| 3.4.19.7 | N-Formylmethionyl oligopeptides + H2O | the presence of this enzyme in intestinal mucosa can prevent absorption of intact bioactive potent inflammatory N-formylmethionyl oligopeptides produced by commensal bacteria in the gut lumen | Rattus norvegicus | ? | - |
? | |
| 3.4.19.7 | Natural formylmethionine oligopeptide from E. coli culture supernatants + H2O | loss of bioactivity and immuno-reactivity | Rattus norvegicus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.19.1 | tetramer | 4 * 82000-84000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.7 | 37 | - |
assay at | Rattus norvegicus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.19.1 | 5.17 | - |
formylmethionine-Leu | - |
Rattus norvegicus | |
| 3.4.19.1 | 5.45 | - |
N-acetyl-Met-Leu | - |
Rattus norvegicus | |
| 3.4.19.1 | 5.94 | - |
formylmethionine-Val | - |
Rattus norvegicus | |
| 3.4.19.1 | 12.3 | - |
formylmethionine-Leu-Phe | - |
Rattus norvegicus | |
| 3.4.19.1 | 15.5 | - |
formylmethionine-Leu-Tyr | - |
Rattus norvegicus | |
| 3.4.19.1 | 27.1 | - |
N-acetyl-Met-Leu-Phe | - |
Rattus norvegicus | |
| 3.4.19.1 | 31.3 | - |
formylmethionine-Trp | - |
Rattus norvegicus | |
| 3.4.19.1 | 70.2 | - |
formylmethionine-Phe | - |
Rattus norvegicus | |
| 3.4.19.1 | 76.4 | - |
formylmethionine-beta-naphthylamide | - |
Rattus norvegicus | |
| 3.4.19.7 | 5.17 | - |
N-Formyl-Met-Leu | - |
Rattus norvegicus | |
| 3.4.19.7 | 5.45 | - |
Acetyl-Met-Leu | - |
Rattus norvegicus | |
| 3.4.19.7 | 5.94 | - |
N-Formyl-Met-Val | - |
Rattus norvegicus | |
| 3.4.19.7 | 12.3 | - |
N-Formyl-Met-Leu-Phe | - |
Rattus norvegicus | |
| 3.4.19.7 | 15.5 | - |
N-Formyl-Met-Leu-Tyr | - |
Rattus norvegicus | |
| 3.4.19.7 | 27.1 | - |
Acetyl-Met-Leu-Phe | - |
Rattus norvegicus | |
| 3.4.19.7 | 31.3 | - |
N-Formyl-Met-Trp | - |
Rattus norvegicus | |
| 3.4.19.7 | 70.2 | - |
N-Formyl-Met-Phe | - |
Rattus norvegicus | |
| 3.4.19.7 | 76.4 | - |
N-Formyl-Met 2-naphthylamide | - |
Rattus norvegicus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.1 | 7.5 | 9 | hydrolysis of formylmethionine-beta-naphthylamide or Met-Met-Leu-Phe | Rattus norvegicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
