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Literature summary extracted from

  • Boffa, M.B.; Wang, W.; Bajzar, l.; Nesheim, M.E.
    Plasma and recombinant thrombin-activable fibrinolysis inhibitor (TAFI) and activated TAFI compared with respsct to glycosylation, thrombin/thrombomodulin-dependent activation, thermal stability, and enzymatic properties (1998), J. Biol. Chem., 273, 2127-2135.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.17.20 thrombin activation Homo sapiens
3.4.17.20 Thrombomodulin activation Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.17.20 expression in BHK-cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.17.20 2-Guanidinoethylmercaptosuccinic acid i.e. GEMSA Homo sapiens
3.4.17.20 6-aminohexanoic acid
-
 Homo sapiens
3.4.17.20 potato carboxypeptidase inhibitor
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.17.20 3.41
-
 N-[3-(2-Furylacryloyl)]-L-Ala-L-Lys recombinant enzyme Homo sapiens
3.4.17.20 4.71
-
 N-[3-(2-Furylacryloyl)]-L-Ala-L-Lys plasma-derived enzyme Homo sapiens
3.4.17.20 766
-
 Hippuryl-L-Lys plasma-derived enzyme Homo sapiens
3.4.17.20 783
-
 hippuryl-L-Arg recombinant enzyme Homo sapiens
3.4.17.20 817
-
 hippuryl-L-Arg plasma-derived enzyme Homo sapiens
3.4.17.20 933
-
 Hippuryl-L-Lys recombinant enzyme Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.17.20 12000
-
 x * 25000 + x * 12000, derived by cleavage at Arg330, inactive form, SDS-PAGE Homo sapiens
3.4.17.20 25000
-
 x * 25000 + x * 12000, derived by cleavage at Arg330, inactive form, SDS-PAGE Homo sapiens
3.4.17.20 35000
-
 x * 35000, active enzyme Homo sapiens
3.4.17.20 46000
-
 x * 46000, enzymes rTAFI and pTAFI, deglycosylated enzymes, slight differences in MW might be due to differences in glycosylation, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.17.20 Fibrin, partially degraded + H2O Homo sapiens
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.20 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.17.20 of cloned enzyme from BHK-cells Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.17.20 plasma
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.17.20 0.017
-
 substrate hippuryl-L-Arg Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.20 Fibrin, partially degraded + H2O
-
 Homo sapiens Fibrin, partially degraded + L-Lys + L-Arg
-
 ?
3.4.17.20 Fibrin, partially degraded + H2O
-
 Homo sapiens ?
-
 ?
3.4.17.20 Hippuryl-L-Arg + H2O
-
 Homo sapiens Hippuric acid + L-Arg
-
 ?
3.4.17.20 Hippuryl-L-Lys + H2O
-
 Homo sapiens Hippuric acid + L-Lys
-
 ?
3.4.17.20 N-[3-(2-Furylacryloyl)]-L-Ala-L-Lys + H2O
-
 Homo sapiens N-[3-(2-Furylacryloyl)]-L-Ala + L-Lys
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.17.20 ? derived from zymogen by cleavage at Arg92, SDS-PAGE Homo sapiens
3.4.17.20 ? x * 25000 + x * 12000, derived by cleavage at Arg330, inactive form, SDS-PAGE Homo sapiens
3.4.17.20 ? x * 46000, enzymes rTAFI and pTAFI, deglycosylated enzymes, slight differences in MW might be due to differences in glycosylation, SDS-PAGE Homo sapiens
3.4.17.20 ? x * 35000, active enzyme Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.17.20
-
-
 enzyme pTAFI and rTAFI stable Homo sapiens
3.4.17.20 22
-
 half-life 120-150 min Homo sapiens
3.4.17.20 30
-
 half-life 40-50 min Homo sapiens
3.4.17.20 37
-
 half-life 8-9 min, stable in presence of inhibitors 2-guanidinoethylmercaptosuccinic acid or 6-aminohexanoic acid Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.17.20 40.7
-
 Hippuryl-L-Lys plasma-derived enzyme Homo sapiens
3.4.17.20 45.6
-
 Hippuryl-L-Lys recombinant enzyme Homo sapiens
3.4.17.20 52.4
-
 hippuryl-L-Arg plasma-derived enzyme Homo sapiens
3.4.17.20 56.7
-
 hippuryl-L-Arg recombinant enzyme Homo sapiens
3.4.17.20 248
-
 N-[3-(2-Furylacryloyl)]-L-Ala-L-Lys recombinant enzyme Homo sapiens
3.4.17.20 293
-
 N-[3-(2-Furylacryloyl)]-L-Ala-L-Lys plasma-derived enzyme Homo sapiens