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Literature summary extracted from

  • Li, Z.S.; Szczypka, M.; Lu, Y.P.; Thiele ,D.J.; Rea, P.A.
    The yeast cadmium factor protein (YCF1) is a vacuolar glutathione S-conjugate pump (1996), J. Biol. Chem., 217, 6509-6517.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.2.2.2 vanadate IC50: 0.18 mM Saccharomyces cerevisiae
7.2.2.2 verapamil IC50: 0.2 mM Saccharomyces cerevisiae
7.2.2.2 vinblastine IC50: 0.08 mM Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.2.2.2 0.000758
-
 leukotriene C4 [side 1] for Sf/YCF1-His vesicles Saccharomyces cerevisiae
7.2.2.2 0.00082
-
 leukotriene C4 [side 1]
-
 Saccharomyces cerevisiae
7.2.2.2 0.055
-
 ATP
-
 Saccharomyces cerevisiae
7.2.2.2 0.094
-
 ATP for Sf/YCF1-His vesicles Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.2.2 tonoplast
-
 Saccharomyces cerevisiae
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.2.2.2 additional information
-
 1515 amino acids Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.2.2.2 Cd/cytoplasm + ATP + H2O Saccharomyces cerevisiae
-
 Cd/vacuole + ADP + phosphate
-
 ?
7.2.2.2 S-(2,4-dinitrophenyl)glutathione/cytoplasm + ATP + H2O Saccharomyces cerevisiae
-
 S-(2,4-dinitrophenyl)glutathione/vacuole + ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.2.2.2 Saccharomyces cerevisiae
-
-
-

Storage Stability

EC Number Storage Stability Organism
7.2.2.2 -85°C, vacuolar membrane preparations Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.2.2 ATP + H2O + leukotriene C4 [side 1]
-
 Saccharomyces cerevisiae ADP + phosphate + leukotriene C4 [side 2]
-
 ?
7.2.2.2 Cd/cytoplasm + ATP + H2O
-
 Saccharomyces cerevisiae Cd/vacuole + ADP + phosphate
-
 ?
7.2.2.2 glutathione S-conjugated leukotriene C4/cytoplasm + ATP + H2O
-
 Saccharomyces cerevisiae glutathione S-conjugated leukotriene C4/vacuole + ADP + phosphate
-
 ?
7.2.2.2 S-(2,4-dinitrophenyl)glutathione/cytoplasm + ATP + H2O
-
 Saccharomyces cerevisiae S-(2,4-dinitrophenyl)glutathione/vacuole + ADP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.2.2.2 Ycf1p
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.2.2.2 25
-
 assay at Saccharomyces cerevisiae

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
7.2.2.2 0.08
-
 IC50: 0.08 mM Saccharomyces cerevisiae vinblastine
7.2.2.2 0.18
-
 IC50: 0.18 mM Saccharomyces cerevisiae vanadate
7.2.2.2 0.2
-
 IC50: 0.2 mM Saccharomyces cerevisiae verapamil