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Literature summary extracted from

  • Li, J.; Schiff, J.A.
    Purification and properties of adenosine 5'-phosphosulphate sulphotransferase from Euglena (1991), Biochem. J., 274, 355-360.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.2.B1 EDTA
-
 Euglena sp.
2.8.2.B1 EDTA 2.0 mM, 1.7fold stimulation Euglena gracilis

General Stability

EC Number General Stability Organism
2.8.2.B1 adenosine 5'-phosphoramidate protects the enzyme from progressive inactivation by thiol substrates Euglena gracilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.B1 2,3-Dimercaptopropan-1-ol
-
 Euglena sp.
2.8.2.B1 2-mercaptoethanol
-
 Euglena sp.
2.8.2.B1 adenosine 5'-phosphoramidate 0.5 mM, inhibits after 2 min incubation, activates after 20 min incubation Euglena gracilis
2.8.2.B1 adenosine 5'-sulfate 2.0 mM, inhibits after 2 min incubation, activates after 20 min incubation Euglena gracilis
2.8.2.B1 AMP 2.0 mM, inhibits after 2 min incubation, activates after 20 min incubation Euglena gracilis
2.8.2.B1 CoCl2 2 mM, 50% inhibition Euglena gracilis
2.8.2.B1 Cu2+
-
 Euglena sp.
2.8.2.B1 CuCl2 0.1 mM, 50% inhibition Euglena gracilis
2.8.2.B1 cysteamine hydrochloride
-
 Euglena sp.
2.8.2.B1 dithioerythritol
-
 Euglena sp.
2.8.2.B1 dithiothreitol
-
 Euglena sp.
2.8.2.B1 Fe2+
-
 Euglena sp.
2.8.2.B1 FeCl2 2 mM, 50% inhibition Euglena gracilis
2.8.2.B1 Hg2+
-
 Euglena sp.
2.8.2.B1 HgCl2 0.1 mM, 50% inhibition Euglena gracilis
2.8.2.B1 KCN
-
 Euglena sp.
2.8.2.B1 L-cysteine
-
 Euglena sp.
2.8.2.B1 L-glutathione
-
 Euglena sp.
2.8.2.B1 L-homocysteine
-
 Euglena sp.
2.8.2.B1 SO32-
-
 Euglena sp.
2.8.2.B1 Sodium thioglycollate
-
 Euglena sp.
2.8.2.B1 sodium thiosulfate
-
 Euglena sp.
2.8.2.B1 Thioacetamide
-
 Euglena sp.
2.8.2.B1 Zn2+
-
 Euglena sp.
2.8.2.B1 ZnCl2 2 mM, 50% inhibition Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.B1 0.0001
-
 adenosine 5'-phosphosulfate 25°C, pH 9.0, reaction with dithiothreitol Euglena gracilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.B1 mitochondrial inner membrane periplasmic side Euglena sp. 5743
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.B1 adenosine 5'-phosphoramidate 0.5 mM, inhibits after 2 min incubation, activates after 20 min incubation. Inhibits by decreasing the initial velocity of the reaction as adenosine 5'-phosphoramidate concentration is increased Euglena gracilis
2.8.2.B1 Ba2+
-
 Euglena sp.
2.8.2.B1 BaCl2 30 mM, 3fold stimulation Euglena gracilis
2.8.2.B1 Ca2+
-
 Euglena sp.
2.8.2.B1 CaCl2 30 mM, 3fold stimulation Euglena gracilis
2.8.2.B1 Mg2+
-
 Euglena sp.
2.8.2.B1 MgCl2 30 mM, 3fold stimulation Euglena gracilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.B1 120000
-
 gel filtration Euglena gracilis
2.8.2.B1 120000
-
 gel filtration Euglena sp.

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.B1 Euglena gracilis
-
 Klebs var. bacillaris mutant W10BSmL
-
2.8.2.B1 Euglena sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.B1
-
 Euglena gracilis
2.8.2.B1
-
 Euglena sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.B1 0.14
-
-
 Euglena sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.B1 adenosine 5'-phosphosulfate + dithioerythritol
-
 Euglena gracilis sulfite + ?
-
 ?
2.8.2.B1 adenosine 5'-phosphosulfate + dithiothreitol
-
 Euglena gracilis sulfite + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.B1 tetramer
-
 Euglena sp.
2.8.2.B1 tetramer held together by covalent (probably disulphide) bonds Euglena gracilis

Synonyms

EC Number Synonyms Comment Organism
2.8.2.B1 adenosine 5'-phosphosulfate sulfotransferase
-
 Euglena gracilis
2.8.2.B1 APSST
-
 Euglena gracilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.B1 25
-
-
 Euglena sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.B1 9
-
-
 Euglena sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.8.2.B1 Euglena gracilis isoelectric focusing, the enzyme may exist in multiple forms having different pI values 5.5 5