BRENDA - Enzyme Database

Characterization of an oligosaccharide-pyrophosphodolichol pyrophosphatase activity in yeast

Belard, M.; Cacan, R.; Verbert,A.; Biochem. J. 255, 235-242 (1988)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.6.1.44
Triton X-100
6fold increase at 0.5%
Saccharomyces cerevisiae
3.6.1.44
Triton X-114
7fold increase at 0.5%
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.44
bacitracin
80% inhibition at 10 mM
Saccharomyces cerevisiae
3.6.1.44
diphosphate
total inhibition at 10 mM
Saccharomyces cerevisiae
3.6.1.44
NAD+
total inhibition at 10 mM
Saccharomyces cerevisiae
3.6.1.44
phosphate
35% inhibition at 10 mM
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.6.1.44
microsome
-
Saccharomyces cerevisiae
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.44
Ca2+
150% activity compared to control, 10 mM
Saccharomyces cerevisiae
3.6.1.44
Mg2+
113% activity compared to control, 10 mM
Saccharomyces cerevisiae
3.6.1.44
Mn2+
149.5% activity compared to control, 10 mM
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.44
oligosaccharide-diphosphodolichol + H2O
Saccharomyces cerevisiae
-
oligosaccharide phosphate + dolichyl phosphate
-
Saccharomyces cerevisiae
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.6.1.44
Saccharomyces cerevisiae
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.44
oligosaccharide-diphosphodolichol + H2O
-
289083
Saccharomyces cerevisiae
oligosaccharide phosphate + dolichyl phosphate
-
289083
Saccharomyces cerevisiae
?
3.6.1.44
oligosaccharide-diphosphodolichol + H2O
oligosaccharide-diphosphodolichol from mouse splenocytes
289083
Saccharomyces cerevisiae
oligosaccharide phosphate + dolichyl phosphate
-
289083
Saccharomyces cerevisiae
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.6.1.44
30
35
no activity at 0°C
Saccharomyces cerevisiae
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.6.1.44
50
-
preincubation for 10 min, half-life at 62°C 1 hour, activity lost after incubation at 70°C
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.44
7
7.5
no activity above pH 9.0
Saccharomyces cerevisiae
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.6.1.44
7
7.5
-
Saccharomyces cerevisiae
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.6.1.44
Triton X-100
6fold increase at 0.5%
Saccharomyces cerevisiae
3.6.1.44
Triton X-114
7fold increase at 0.5%
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.44
bacitracin
80% inhibition at 10 mM
Saccharomyces cerevisiae
3.6.1.44
diphosphate
total inhibition at 10 mM
Saccharomyces cerevisiae
3.6.1.44
NAD+
total inhibition at 10 mM
Saccharomyces cerevisiae
3.6.1.44
phosphate
35% inhibition at 10 mM
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.6.1.44
microsome
-
Saccharomyces cerevisiae
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.44
Ca2+
150% activity compared to control, 10 mM
Saccharomyces cerevisiae
3.6.1.44
Mg2+
113% activity compared to control, 10 mM
Saccharomyces cerevisiae
3.6.1.44
Mn2+
149.5% activity compared to control, 10 mM
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.44
oligosaccharide-diphosphodolichol + H2O
Saccharomyces cerevisiae
-
oligosaccharide phosphate + dolichyl phosphate
-
Saccharomyces cerevisiae
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.44
oligosaccharide-diphosphodolichol + H2O
-
289083
Saccharomyces cerevisiae
oligosaccharide phosphate + dolichyl phosphate
-
289083
Saccharomyces cerevisiae
?
3.6.1.44
oligosaccharide-diphosphodolichol + H2O
oligosaccharide-diphosphodolichol from mouse splenocytes
289083
Saccharomyces cerevisiae
oligosaccharide phosphate + dolichyl phosphate
-
289083
Saccharomyces cerevisiae
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.6.1.44
30
35
no activity at 0°C
Saccharomyces cerevisiae
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.6.1.44
50
-
preincubation for 10 min, half-life at 62°C 1 hour, activity lost after incubation at 70°C
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.44
7
7.5
no activity above pH 9.0
Saccharomyces cerevisiae
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.6.1.44
7
7.5
-
Saccharomyces cerevisiae