BRENDA - Enzyme Database

Intracellular distribution and solubilization of yeast dolichylpyrophoshatase

Shabalin, Y.A.; Hamut, A.; Bukhtiyarov, Y.E.; Kulaev, I.S.; Biochemistry (Moscow) 56, 2032-2041 (1991)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.6.1.43
EDTA
-
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.43
divalent cations
strong or complete inhibition at concentrations of 5 mM for all tested ions
Saccharomyces cerevisiae
3.6.1.43
phosphate
strong inhibition, 5 mM causes more than 60% loss of activity
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.6.1.43
membrane
-
Saccharomyces cerevisiae
16020
-
3.6.1.43
microsome
-
Saccharomyces cerevisiae
-
-
3.6.1.43
vacuole
-
Saccharomyces cerevisiae
5773
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.43
dolichyl diphosphate + H2O
Saccharomyces cerevisiae
involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
dolichyl phosphate + phosphate
-
Saccharomyces cerevisiae
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.6.1.43
Saccharomyces cerevisiae
-
-
-
Storage Stability
EC Number
Storage Stability
Organism
3.6.1.43
-10°C solubilized enzyme can be stored for 6 months without loss of activity
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.43
dolichyl diphosphate + H2O
-
289082
Saccharomyces cerevisiae
dolichyl phosphate + phosphate
-
289082
Saccharomyces cerevisiae
?
3.6.1.43
dolichyl diphosphate + H2O
involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
289082
Saccharomyces cerevisiae
dolichyl phosphate + phosphate
-
289082
Saccharomyces cerevisiae
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.6.1.43
EDTA
-
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.43
divalent cations
strong or complete inhibition at concentrations of 5 mM for all tested ions
Saccharomyces cerevisiae
3.6.1.43
phosphate
strong inhibition, 5 mM causes more than 60% loss of activity
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.6.1.43
membrane
-
Saccharomyces cerevisiae
16020
-
3.6.1.43
microsome
-
Saccharomyces cerevisiae
-
-
3.6.1.43
vacuole
-
Saccharomyces cerevisiae
5773
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.43
dolichyl diphosphate + H2O
Saccharomyces cerevisiae
involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
dolichyl phosphate + phosphate
-
Saccharomyces cerevisiae
?
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.6.1.43
-10°C solubilized enzyme can be stored for 6 months without loss of activity
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.43
dolichyl diphosphate + H2O
-
289082
Saccharomyces cerevisiae
dolichyl phosphate + phosphate
-
289082
Saccharomyces cerevisiae
?
3.6.1.43
dolichyl diphosphate + H2O
involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
289082
Saccharomyces cerevisiae
dolichyl phosphate + phosphate
-
289082
Saccharomyces cerevisiae
?