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Literature summary extracted from

  • Scher, M.G.; Waechter, C.J.
    Brain dolichyl pyrophosphate phosphatase. Solubilization, characterization, and differentiation from dolichyl monophosphate phosphatase activity (1984), J. Biol. Chem., 259, 14580-14585.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.43 diphosphate
-
 Bos taurus
3.6.1.43 EDTA
-
 Bos taurus
3.6.1.43 phosphate
-
 Bos taurus
3.6.1.43 Trimetaphosphate
-
 Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.43 bacitracin complete inhibition Bos taurus
3.6.1.43 Ca2+
-
 Bos taurus
3.6.1.43 farnesol very slight inhibition Bos taurus
3.6.1.43 farnesyl diphosphate
-
 Bos taurus
3.6.1.43 isopentenyl diphosphate
-
 Bos taurus
3.6.1.43 Mn2+ strong inhibitor Bos taurus
3.6.1.43 NaF slight inhibition Bos taurus
3.6.1.43 phosphate activating Bos taurus
3.6.1.43 undecaprenyl diphosphate
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.43 0.02
-
 dolichyl diphosphate
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.43 membrane integral Bos taurus 16020
-
3.6.1.43 microsome
-
 Bos taurus
-
-
3.6.1.43 mitochondrial outer membrane integral Bos taurus 5741
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.43 Ca2+ inhibitory Bos taurus
3.6.1.43 Mn2+ strong inhibitor Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.43 dolichyl diphosphate + H2O Bos taurus involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate dolichyl phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.43 Bos taurus
-
 calf
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.43 brain
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.43 0.0031
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.6.1.43 -17°C stable one month Bos taurus
3.6.1.43 4°C solubilized enzyme stable overnight Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.43 dolichyl diphosphate + H2O
-
 Bos taurus dolichyl phosphate + phosphate
-
 ?
3.6.1.43 dolichyl diphosphate + H2O involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate Bos taurus dolichyl phosphate + phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.43 50
-
 2 min causes 80-85% loss of activity Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.43 7
-
 optimum activity at neutral pH Bos taurus