BRENDA - Enzyme Database

Brain dolichyl pyrophosphate phosphatase. Solubilization, characterization, and differentiation from dolichyl monophosphate phosphatase activity

Scher, M.G.; Waechter, C.J.; J. Biol. Chem. 259, 14580-14585 (1984)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.6.1.43
diphosphate
-
Bos taurus
3.6.1.43
EDTA
-
Bos taurus
3.6.1.43
phosphate
-
Bos taurus
3.6.1.43
Trimetaphosphate
-
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.43
bacitracin
complete inhibition
Bos taurus
3.6.1.43
Ca2+
-
Bos taurus
3.6.1.43
farnesol
very slight inhibition
Bos taurus
3.6.1.43
farnesyl diphosphate
-
Bos taurus
3.6.1.43
isopentenyl diphosphate
-
Bos taurus
3.6.1.43
Mn2+
strong inhibitor
Bos taurus
3.6.1.43
NaF
slight inhibition
Bos taurus
3.6.1.43
phosphate
activating
Bos taurus
3.6.1.43
undecaprenyl diphosphate
-
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.43
0.02
-
dolichyl diphosphate
-
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.6.1.43
membrane
integral
Bos taurus
16020
-
3.6.1.43
microsome
-
Bos taurus
-
-
3.6.1.43
mitochondrial outer membrane
integral
Bos taurus
5741
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.43
Ca2+
inhibitory
Bos taurus
3.6.1.43
Mn2+
strong inhibitor
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.43
dolichyl diphosphate + H2O
Bos taurus
involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
dolichyl phosphate + phosphate
-
Bos taurus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.6.1.43
Bos taurus
-
calf
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.6.1.43
brain
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.6.1.43
0.0031
-
-
Bos taurus
Storage Stability
EC Number
Storage Stability
Organism
3.6.1.43
-17C stable one month
Bos taurus
3.6.1.43
4C solubilized enzyme stable overnight
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.43
dolichyl diphosphate + H2O
-
289079
Bos taurus
dolichyl phosphate + phosphate
-
289079
Bos taurus
?
3.6.1.43
dolichyl diphosphate + H2O
involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
289079
Bos taurus
dolichyl phosphate + phosphate
-
289079
Bos taurus
?
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.6.1.43
50
-
2 min causes 80-85% loss of activity
Bos taurus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.43
7
-
optimum activity at neutral pH
Bos taurus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.6.1.43
diphosphate
-
Bos taurus
3.6.1.43
EDTA
-
Bos taurus
3.6.1.43
phosphate
-
Bos taurus
3.6.1.43
Trimetaphosphate
-
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.43
bacitracin
complete inhibition
Bos taurus
3.6.1.43
Ca2+
-
Bos taurus
3.6.1.43
farnesol
very slight inhibition
Bos taurus
3.6.1.43
farnesyl diphosphate
-
Bos taurus
3.6.1.43
isopentenyl diphosphate
-
Bos taurus
3.6.1.43
Mn2+
strong inhibitor
Bos taurus
3.6.1.43
NaF
slight inhibition
Bos taurus
3.6.1.43
phosphate
activating
Bos taurus
3.6.1.43
undecaprenyl diphosphate
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.43
0.02
-
dolichyl diphosphate
-
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.6.1.43
membrane
integral
Bos taurus
16020
-
3.6.1.43
microsome
-
Bos taurus
-
-
3.6.1.43
mitochondrial outer membrane
integral
Bos taurus
5741
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.43
Ca2+
inhibitory
Bos taurus
3.6.1.43
Mn2+
strong inhibitor
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.43
dolichyl diphosphate + H2O
Bos taurus
involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
dolichyl phosphate + phosphate
-
Bos taurus
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.6.1.43
brain
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.6.1.43
0.0031
-
-
Bos taurus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.6.1.43
-17C stable one month
Bos taurus
3.6.1.43
4C solubilized enzyme stable overnight
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.43
dolichyl diphosphate + H2O
-
289079
Bos taurus
dolichyl phosphate + phosphate
-
289079
Bos taurus
?
3.6.1.43
dolichyl diphosphate + H2O
involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
289079
Bos taurus
dolichyl phosphate + phosphate
-
289079
Bos taurus
?
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.6.1.43
50
-
2 min causes 80-85% loss of activity
Bos taurus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.43
7
-
optimum activity at neutral pH
Bos taurus