BRENDA - Enzyme Database

Methylene and halomethylene analogues of diadenosine 5'5'''-P1,P3-triphosphate (ApppA) as substrates or inhibitors of ApppA-degrading enzymes

Guranowski, A.; Starzynska, E.; Gzik, L.; Langston, S.P.; Brown, P.; Blackburn, G.M.; Nucleosides Nucleotides 14, 731-734 (1995)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate with CCl2-bridge between P1 and P2
i.e. ApCCl2ppA
Acanthamoeba castellanii
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate with CHCl-bridge between P1 and P2
i.e. ApCHClppA
Acanthamoeba castellanii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.53
0.0035
-
P1,P3-bis(5'-adenosyl) triphosphate with CHF-bridge between P1 and P2
pH 8.0, 30°C
Acanthamoeba castellanii
2.7.7.53
0.004
-
P1,P3-bis(5'-adenosyl) triphosphate with CF2-bridge between P1 and P2
pH 8.0, 30°C
Acanthamoeba castellanii
2.7.7.53
0.009
-
P1,P3-bis(5'-adenosyl) triphosphate
pH 8.0, 30°C
Acanthamoeba castellanii
2.7.7.53
0.012
-
P1,P3-bis(5'-adenosyl) triphosphate with CH2-bridge between P1 and P2
pH 8.0, 30°C
Acanthamoeba castellanii
3.6.1.29
0.001
-
P1-P3-bis(5'-adenosyl) triphosphate
-
Lupinus luteus
3.6.1.29
0.09
-
ApCHFppA
-
Lupinus luteus
3.6.1.29
0.117
-
ApCH2ppA
-
Lupinus luteus
3.6.1.29
0.212
-
ApCF2ppA
-
Lupinus luteus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.29
P1-P3-bis(5'-adenosyl) triphosphate + H2O
Lupinus luteus
-
ADP + AMP
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.53
Acanthamoeba castellanii
-
-
-
3.6.1.29
Lupinus luteus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.7.53
partial
Acanthamoeba castellanii
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.6.1.29
seed
-
Lupinus luteus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.53
additional information
enzyme splits the anhydride bond between the alpha and beta phosphates by phosphorolysis
289064
Acanthamoeba castellanii
?
-
-
-
-
2.7.7.53
additional information
no activity with P1,P3-bis(5'-adenosyl) triphosphate with CH2-bridge between P1and P2 and between P3 and P4, i.e. ApCH2pCH2pA
289064
Acanthamoeba castellanii
?
-
-
-
-
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
preferred substrate
289064
Acanthamoeba castellanii
ADP
-
-
-
?
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
i.e. AP3A
289064
Acanthamoeba castellanii
ADP
-
-
-
?
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate with CF2-bridge between P1 and P2 + phosphate
low activity
289064
Acanthamoeba castellanii
?
-
-
-
?
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate with CF2-bridge between P1 and P2 + phosphate
ApCF2ppA, difluoromethylene group substituting the oxygen
289064
Acanthamoeba castellanii
?
-
-
-
?
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate with CH2-bridge between P1 and P2 + phosphate
poor substrate
289064
Acanthamoeba castellanii
?
-
-
-
?
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate with CH2-bridge between P1 and P2 + phosphate
i.e. ApCH2ppA, methylene group substituting the oxygen
289064
Acanthamoeba castellanii
?
-
-
-
?
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate with CHF-bridge between P1 and P2 + phosphate
low activity
289064
Acanthamoeba castellanii
?
-
-
-
?
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate with CHF-bridge between P1 and P2 + phosphate
i.e. ApCHFppA, fluoromethylene group substituting the oxygen
289064
Acanthamoeba castellanii
?
-
-
-
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
289064
Acanthamoeba castellanii
ADP + ATP
-
289064
Acanthamoeba castellanii
?
3.6.1.29
ApCF2ppA
-
289064
Lupinus luteus
?
-
-
-
?
3.6.1.29
ApCH2ppA
-
289064
Lupinus luteus
?
-
-
-
?
3.6.1.29
ApCHFppA
-
289064
Lupinus luteus
?
-
-
-
?
3.6.1.29
P1-P3-bis(5'-adenosyl) triphosphate + H2O
-
289064
Lupinus luteus
ADP + AMP
-
-
-
?
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.7.53
0.008
-
P1,P3-bis(5'-adenosyl) triphosphate with CHCl-bridge between P1 and P2
pH 8.0, 30°C
Acanthamoeba castellanii
2.7.7.53
0.014
-
P1,P3-bis(5'-adenosyl) triphosphate with CCl2-bridge between P1 and P2
pH 8.0, 30°C
Acanthamoeba castellanii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate with CCl2-bridge between P1 and P2
i.e. ApCCl2ppA
Acanthamoeba castellanii
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate with CHCl-bridge between P1 and P2
i.e. ApCHClppA
Acanthamoeba castellanii
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.7.53
0.008
-
P1,P3-bis(5'-adenosyl) triphosphate with CHCl-bridge between P1 and P2
pH 8.0, 30°C
Acanthamoeba castellanii
2.7.7.53
0.014
-
P1,P3-bis(5'-adenosyl) triphosphate with CCl2-bridge between P1 and P2
pH 8.0, 30°C
Acanthamoeba castellanii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.53
0.0035
-
P1,P3-bis(5'-adenosyl) triphosphate with CHF-bridge between P1 and P2
pH 8.0, 30°C
Acanthamoeba castellanii
2.7.7.53
0.004
-
P1,P3-bis(5'-adenosyl) triphosphate with CF2-bridge between P1 and P2
pH 8.0, 30°C
Acanthamoeba castellanii
2.7.7.53
0.009
-
P1,P3-bis(5'-adenosyl) triphosphate
pH 8.0, 30°C
Acanthamoeba castellanii
2.7.7.53
0.012
-
P1,P3-bis(5'-adenosyl) triphosphate with CH2-bridge between P1 and P2
pH 8.0, 30°C
Acanthamoeba castellanii
3.6.1.29
0.001
-
P1-P3-bis(5'-adenosyl) triphosphate
-
Lupinus luteus
3.6.1.29
0.09
-
ApCHFppA
-
Lupinus luteus
3.6.1.29
0.117
-
ApCH2ppA
-
Lupinus luteus
3.6.1.29
0.212
-
ApCF2ppA
-
Lupinus luteus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.29
P1-P3-bis(5'-adenosyl) triphosphate + H2O
Lupinus luteus
-
ADP + AMP
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.7.53
partial
Acanthamoeba castellanii
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.6.1.29
seed
-
Lupinus luteus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.53
additional information
enzyme splits the anhydride bond between the alpha and beta phosphates by phosphorolysis
289064
Acanthamoeba castellanii
?
-
-
-
-
2.7.7.53
additional information
no activity with P1,P3-bis(5'-adenosyl) triphosphate with CH2-bridge between P1and P2 and between P3 and P4, i.e. ApCH2pCH2pA
289064
Acanthamoeba castellanii
?
-
-
-
-
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
preferred substrate
289064
Acanthamoeba castellanii
ADP
-
-
-
?
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
i.e. AP3A
289064
Acanthamoeba castellanii
ADP
-
-
-
?
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate with CF2-bridge between P1 and P2 + phosphate
low activity
289064
Acanthamoeba castellanii
?
-
-
-
?
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate with CF2-bridge between P1 and P2 + phosphate
ApCF2ppA, difluoromethylene group substituting the oxygen
289064
Acanthamoeba castellanii
?
-
-
-
?
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate with CH2-bridge between P1 and P2 + phosphate
poor substrate
289064
Acanthamoeba castellanii
?
-
-
-
?
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate with CH2-bridge between P1 and P2 + phosphate
i.e. ApCH2ppA, methylene group substituting the oxygen
289064
Acanthamoeba castellanii
?
-
-
-
?
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate with CHF-bridge between P1 and P2 + phosphate
low activity
289064
Acanthamoeba castellanii
?
-
-
-
?
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate with CHF-bridge between P1 and P2 + phosphate
i.e. ApCHFppA, fluoromethylene group substituting the oxygen
289064
Acanthamoeba castellanii
?
-
-
-
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
289064
Acanthamoeba castellanii
ADP + ATP
-
289064
Acanthamoeba castellanii
?
3.6.1.29
ApCF2ppA
-
289064
Lupinus luteus
?
-
-
-
?
3.6.1.29
ApCH2ppA
-
289064
Lupinus luteus
?
-
-
-
?
3.6.1.29
ApCHFppA
-
289064
Lupinus luteus
?
-
-
-
?
3.6.1.29
P1-P3-bis(5'-adenosyl) triphosphate + H2O
-
289064
Lupinus luteus
ADP + AMP
-
-
-
?