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Literature summary extracted from

  • Moore, J.T.; Ciesla, J.M.; Changchien, L.M.; Maley, G.F.; Maley, F.
    Identification of a site necessary for allosteric regulation in T4-phage deoxycytidylate deaminase (1994), Biochemistry, 33, 2104-2112.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.4.12 5-hydroxymethyl-dCTP natural positive allosteric effector, enzyme is much more effectively regulated by its natural effector, 5-hydroxymethyl-dCTP, than by dCTP, binding of 5-hydroxymethyl-dCTP is much more pH dependent than dCTP Tequatrovirus T4
3.5.4.12 dCTP positive allosteric effector Homo sapiens
3.5.4.12 dCTP positive allosteric effector Tequatrovirus T4
3.5.4.12 dCTP wild-type, dCTP required for activity, no activity in absence of dCTP and Mg2+ Tequatrovirus T4
3.5.4.12 dCTP mutant F112A, dCTP not required Tequatrovirus T4

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.12 cloned and amplified in a high-expression system, expression in Escherichia coli strain BL21-DE3/pLysS Tequatrovirus T4
3.5.4.12 cloned and overexpressed in Escherichia coli strain BL21-DE3/pLysS Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.5.4.12 F112A as active as wild-type even in the absence of dCTP and only weakly inhibited by dTTP, loss of allosteric regulation by dCTP and dTTP, no activation by dCTP, no inhibition by dTTP Tequatrovirus T4

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.12 dTTP allosteric inhibitor Homo sapiens
3.5.4.12 dTTP allosteric inhibitor; mutant F112A, no or very slight inhibition, high concentration, 0.24 mM: slight inhibition Tequatrovirus T4
3.5.4.12 dUMP
-
 Homo sapiens
3.5.4.12 dUMP
-
 Tequatrovirus T4
3.5.4.12 dUTP
-
 Homo sapiens
3.5.4.12 dUTP allosteric inhibitor Tequatrovirus T4

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.12 Zn2+ involved in catalytic mechanism involving His104, and Cys132 and Cys135 Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.12 dCMP + H2O Homo sapiens
-
 dUMP + NH3
-
 ?
3.5.4.12 dCMP + H2O Tequatrovirus T4 critical branchpoint in pyrimidine deoxynucleotide metabolism dUMP + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.12 Homo sapiens
-
 human
-
3.5.4.12 Tequatrovirus T4
-
 wild-type and mutant F112A
-
3.5.4.12 Tequatrovirus T4
-
 T4-phage infected Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.12 cloned wild-type and mutant F112A Tequatrovirus T4

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.4.12 dCMP + H2O = dUMP + NH3 regulation, allosteric end-product regulation Homo sapiens
a
3.5.4.12 dCMP + H2O = dUMP + NH3 regulation, allosteric end-product regulation Tequatrovirus T4
a
3.5.4.12 dCMP + H2O = dUMP + NH3 feedback regulation Homo sapiens
a
3.5.4.12 dCMP + H2O = dUMP + NH3 feedback regulation Tequatrovirus T4
a
3.5.4.12 dCMP + H2O = dUMP + NH3 highly regulated allosteric enzyme Homo sapiens
a
3.5.4.12 dCMP + H2O = dUMP + NH3 highly regulated allosteric enzyme Tequatrovirus T4
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.12 dCMP + H2O
-
 Homo sapiens dUMP + NH3
-
 ?
3.5.4.12 dCMP + H2O
-
 Tequatrovirus T4 dUMP + NH3
-
 ?
3.5.4.12 dCMP + H2O critical branchpoint in pyrimidine deoxynucleotide metabolism Tequatrovirus T4 dUMP + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.12 hexamer 6 identical subunits Homo sapiens
3.5.4.12 hexamer 6 identical subunits Tequatrovirus T4

Synonyms

EC Number Synonyms Comment Organism
3.5.4.12 T4-dCMP deaminase T4-phage infected Tequatrovirus T4
3.5.4.12 T4-deaminase T4-phage infected Tequatrovirus T4
3.5.4.12 T4-phage deoxycytidylate deaminase T4-phage infected Tequatrovirus T4

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.12 30
-
 assay at Homo sapiens
3.5.4.12 30
-
 assay at Tequatrovirus T4