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Literature summary extracted from

  • Nucci, R.; Raia, C.A.; Vaccaro, C.; Rossi, M.; Whitehead, E.P.
    Allosteric modifier and substrate binding of donkey deoxycytidylate aminohydrolase (EC 3.5.4.12) (1991), Arch. Biochem. Biophys., 289, 19-25.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.4.12 dCTP positive allosteric effector Equus asinus
3.5.4.12 additional information mechanism of activation Equus asinus

General Stability

EC Number General Stability Organism
3.5.4.12 glycerol stabilizes Equus asinus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.12 dAMP kinetics of dAMP inhibition Equus asinus
3.5.4.12 dTTP allosteric inhibitor; cooperative inhibition, reversed by dCTP, mechanism of inhibition Equus asinus
3.5.4.12 additional information mechanism of inhibition Equus asinus
3.5.4.12 UTP-adipic hydrazide cooperative inhibition, reversed by dCTP Equus asinus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.12 additional information
-
 additional information cooperative kinetics, kinetic affinities and effects, mechanism Equus asinus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.12 18842
-
 6 * 18842, amino acid analysis Equus asinus
3.5.4.12 113100
-
 amino acid analysis Equus asinus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.12 dCMP + H2O Equus asinus
-
 dUMP + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.12 Equus asinus
-
 donkey
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.12
-
 Equus asinus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.4.12 dCMP + H2O = dUMP + NH3 mechanism Equus asinus
a
3.5.4.12 dCMP + H2O = dUMP + NH3 regulation, allosteric end-product regulation Equus asinus
a
3.5.4.12 dCMP + H2O = dUMP + NH3 feedback regulation Equus asinus
a
3.5.4.12 dCMP + H2O = dUMP + NH3 highly regulated allosteric enzyme Equus asinus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.4.12 spleen
-
 Equus asinus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.12 730
-
-
 Equus asinus

Storage Stability

EC Number Storage Stability Organism
3.5.4.12 -20°C, 0.1 M Na-phosphate, pH 7.5, 50% glycerol, 12 months, no activity lost Equus asinus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.12 5-methyl-dCMP + H2O
-
 Equus asinus 5-methyl-dUMP + NH3
-
 ?
3.5.4.12 dCMP + H2O
-
 Equus asinus dUMP + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.12 hexamer 6 identical subunits Equus asinus
3.5.4.12 hexamer 6 * 18842, amino acid analysis Equus asinus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.12 38
-
 assay at Equus asinus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.4.12 1400
-
 dCMP
-
 Equus asinus