Any feedback?
Literature summary extracted from
- On the regulation of a bacterial deoxycytidylate deaminase (1971), J. Biol. Chem., 246, 7755-7758.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.4.12 | 5-hydroxymethyl-dCTP | 30% activation | Lactobacillus acidophilus |  |
| 3.5.4.12 | dCTP | positive allosteric effector | Staphylococcus aureus | |
| 3.5.4.12 | dCTP | positive allosteric effector | Lactobacillus acidophilus | |
| 3.5.4.12 | dCTP | specifically markedly activated, Km: 0.00017 mM | Lactobacillus acidophilus | |
| 3.5.4.12 | dGTP | very slight activation | Lactobacillus acidophilus | |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.5.4.12 | enzyme is unstable | Lactobacillus acidophilus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.4.12 | 2',3'-di-dTTP | 7% inhibition | Lactobacillus acidophilus | |
| 3.5.4.12 | 5-bromo-dUTP | 92% inhibition | Lactobacillus acidophilus | |
| 3.5.4.12 | dTTP | allosteric inhibitor; specifically markedly inhibited, Ki: 0.0017 mM | Lactobacillus acidophilus | |
| 3.5.4.12 | dTTP | allosteric inhibitor | Staphylococcus aureus | |
| 3.5.4.12 | dUTP | 79% inhibition | Lactobacillus acidophilus | |
| 3.5.4.12 | additional information | no inhibition by dATP, dGTP, 5-bromo-UTP | Lactobacillus acidophilus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.12 | dCMP + H2O | Staphylococcus aureus | - |
dUMP + NH3 | - |
? | |
| 3.5.4.12 | dCMP + H2O | Lactobacillus acidophilus | - |
dUMP + NH3 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.4.12 | Lactobacillus acidophilus | - |
- |
- |
| 3.5.4.12 | Staphylococcus aureus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.4.12 | - |
Staphylococcus aureus |
| 3.5.4.12 | partial | Lactobacillus acidophilus |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.5.4.12 | dCMP + H2O = dUMP + NH3 | regulation, allosteric end-product regulation | Staphylococcus aureus | |
| 3.5.4.12 | dCMP + H2O = dUMP + NH3 | regulation, allosteric end-product regulation | Lactobacillus acidophilus | |
| 3.5.4.12 | dCMP + H2O = dUMP + NH3 | highly regulated allosteric enzyme | Staphylococcus aureus | |
| 3.5.4.12 | dCMP + H2O = dUMP + NH3 | highly regulated allosteric enzyme | Lactobacillus acidophilus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.12 | 0.03 | - |
- |
Lactobacillus acidophilus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.5.4.12 | on ice, 0.05 M Tris, pH 8.0, 20 mM dithiothreitol, stable for weeks | Lactobacillus acidophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.12 | dCMP + H2O | - |
Staphylococcus aureus | dUMP + NH3 | - |
? | |
| 3.5.4.12 | dCMP + H2O | - |
Lactobacillus acidophilus | dUMP + NH3 | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.12 | 37 | - |
assay at | Lactobacillus acidophilus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
